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Molecular Characterization and Expression Profiling of Tomato GRF Transcription Factor Family Genes in Response to Abiotic Stresses and Phytohormones.
Int J Mol Sci. 2017 May 13; 18(5)IJ

Abstract

Growth regulating factors (GRFs) are plant-specific transcription factors that are involved in diverse biological and physiological processes, such as growth, development and stress and hormone responses. However, the roles of GRFs in vegetative and reproductive growth, development and stress responses in tomato (Solanum lycopersicum) have not been extensively explored. In this study, we characterized the 13 SlGRF genes. In silico analysis of protein motif organization, intron-exon distribution, and phylogenetic classification confirmed the presence of GRF proteins in tomato. The tissue-specific expression analysis revealed that most of the SlGRF genes were preferentially expressed in young and growing tissues such as flower buds and meristems, suggesting that SlGRFs are important during growth and development of these tissues. Some of the SlGRF genes were preferentially expressed in fruits at distinct developmental stages suggesting their involvement in fruit development and the ripening process. The strong and differential expression of different SlGRFs under NaCl, drought, heat, cold, abscisic acid (ABA), and jasmonic acid (JA) treatment, predict possible functions for these genes in stress responses in addition to their growth regulatory functions. Further, differential expression of SlGRF genes upon gibberellic acid (GA3) treatment indicates their probable function in flower development and stress responses through a gibberellic acid (GA)-mediated pathway. The results of this study provide a basis for further functional analysis and characterization of this important gene family in tomato.

Authors+Show Affiliations

Department of Agricultural Industry Economy and Education, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-950, Korea. kkr@pstu.ac.bd.Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-950, Korea. gpb21bau@bau.edu.bd.Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-950, Korea. jipark@sunchon.ac.kr.Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-950, Korea. ujjalnath@gmail.com.Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Korea. ckkim@knu.ac.kr.Department of Horticultural Science, Kyungpook National University, Daegu 702-701, Korea. kblim@knu.ac.kr.Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-950, Korea. nis@sunchon.ac.kr.Department of Agricultural Industry Economy and Education, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-950, Korea. queen@sunchon.ac.kr. Department of Agricultural Education, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-950, Korea. queen@sunchon.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28505092

Citation

Khatun, Khadiza, et al. "Molecular Characterization and Expression Profiling of Tomato GRF Transcription Factor Family Genes in Response to Abiotic Stresses and Phytohormones." International Journal of Molecular Sciences, vol. 18, no. 5, 2017.
Khatun K, Robin AHK, Park JI, et al. Molecular Characterization and Expression Profiling of Tomato GRF Transcription Factor Family Genes in Response to Abiotic Stresses and Phytohormones. Int J Mol Sci. 2017;18(5).
Khatun, K., Robin, A. H. K., Park, J. I., Nath, U. K., Kim, C. K., Lim, K. B., Nou, I. S., & Chung, M. Y. (2017). Molecular Characterization and Expression Profiling of Tomato GRF Transcription Factor Family Genes in Response to Abiotic Stresses and Phytohormones. International Journal of Molecular Sciences, 18(5). https://doi.org/10.3390/ijms18051056
Khatun K, et al. Molecular Characterization and Expression Profiling of Tomato GRF Transcription Factor Family Genes in Response to Abiotic Stresses and Phytohormones. Int J Mol Sci. 2017 May 13;18(5) PubMed PMID: 28505092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular Characterization and Expression Profiling of Tomato GRF Transcription Factor Family Genes in Response to Abiotic Stresses and Phytohormones. AU - Khatun,Khadiza, AU - Robin,Arif Hasan Khan, AU - Park,Jong-In, AU - Nath,Ujjal Kumar, AU - Kim,Chang Kil, AU - Lim,Ki-Byung, AU - Nou,Ill Sup, AU - Chung,Mi-Young, Y1 - 2017/05/13/ PY - 2017/03/24/received PY - 2017/05/07/revised PY - 2017/05/09/accepted PY - 2017/5/16/entrez PY - 2017/5/16/pubmed PY - 2018/3/3/medline KW - GRF gene KW - Solanum lycopersicum KW - abiotic stress KW - fruit development KW - organ-specific expression KW - phytohormone treatment JF - International journal of molecular sciences JO - Int J Mol Sci VL - 18 IS - 5 N2 - Growth regulating factors (GRFs) are plant-specific transcription factors that are involved in diverse biological and physiological processes, such as growth, development and stress and hormone responses. However, the roles of GRFs in vegetative and reproductive growth, development and stress responses in tomato (Solanum lycopersicum) have not been extensively explored. In this study, we characterized the 13 SlGRF genes. In silico analysis of protein motif organization, intron-exon distribution, and phylogenetic classification confirmed the presence of GRF proteins in tomato. The tissue-specific expression analysis revealed that most of the SlGRF genes were preferentially expressed in young and growing tissues such as flower buds and meristems, suggesting that SlGRFs are important during growth and development of these tissues. Some of the SlGRF genes were preferentially expressed in fruits at distinct developmental stages suggesting their involvement in fruit development and the ripening process. The strong and differential expression of different SlGRFs under NaCl, drought, heat, cold, abscisic acid (ABA), and jasmonic acid (JA) treatment, predict possible functions for these genes in stress responses in addition to their growth regulatory functions. Further, differential expression of SlGRF genes upon gibberellic acid (GA3) treatment indicates their probable function in flower development and stress responses through a gibberellic acid (GA)-mediated pathway. The results of this study provide a basis for further functional analysis and characterization of this important gene family in tomato. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/28505092/Molecular_Characterization_and_Expression_Profiling_of_Tomato_GRF_Transcription_Factor_Family_Genes_in_Response_to_Abiotic_Stresses_and_Phytohormones_ L2 - https://www.mdpi.com/resolver?pii=ijms18051056 DB - PRIME DP - Unbound Medicine ER -