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Antimicrobial, anticancer, and antioxidant compounds from Premna resinosa growing in Saudi Arabia.
Pharm Biol. 2017 Dec; 55(1):1759-1766.PB

Abstract

CONTEXT

Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage.

OBJECTIVE

This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constituents.

MATERIALS AND METHODS

The methanol extract of P. resinosa aerial parts and its fractions (n-hexane, dichloromethane, ethyl acetate and n-butanol) were tested. Antimicrobial activity was tested using microdilution method against three Gram-positive and four Gram-negative bacteria. The tested concentrations ranged from 4000 to 7.8 μg/mL and MIC values were determined after 24 h incubation. Anticancer activity was evaluated against three human cancer cell lines (Daoy, HepG2 and SK-MEL28) using MTT assay. Antioxidant activity was investigated by DPPH scavenging method and β-carotene-linoleic acid assay.

RESULTS

The greatest antimicrobial activity was exhibited by n-hexane fraction (MIC 10 μg/mL) against Staphylococcus aureus, Enterococcus faecalis, and Shigella flexneri. The n-hexane fraction induced the greatest cytotoxic activity against Daoy, HepG2, and SK-MEL28 cell lines with IC50 values of 9.0, 8.5 and 13.2, respectively. Moreover, the dichloromethane and ethyl acetate fractions showed the highest antioxidant potential. A bioassay-guided fractionation led to the isolation and characterization of seven compounds for the first time, namely, quercetin (1), 3-methoxy quercetin (2), kaempferol (3), 3-methoxy kaempferol (4), myricetin 3,7,3'-trimethyl ether (5), lupeol (6), and stigmasterol (7).

CONCLUSION

Our results indicate that P. resinosa is a source for antimicrobial and cytotoxic compounds. However, further work is required to isolate other active principles and to determine the mechanism of action.

Authors+Show Affiliations

a Department of Natural Products and Alternative Medicine, College of Pharmacy , King Abdulaziz University , Jeddah , Saudi Arabia.b Department of Pharmacognosy, College of Pharmacy , King Saud University , Riyadh , Saudi Arabia. c Department of Pharmacognosy, Faculty of Pharmacy , Sana'a University , Sana'a , Yemen.d Department of Botany and Microbiology, College of Science , King Saud University , Riyadh , Saudi Arabia.e Department of Pharmacology and Toxicology, College of Pharmacy , King Saud University , Riyadh , Saudi Arabia.f Vitiligo Research Chair, College of Medicine , King Saud University , Riyadh , Saudi Arabia.e Department of Pharmacology and Toxicology, College of Pharmacy , King Saud University , Riyadh , Saudi Arabia.b Department of Pharmacognosy, College of Pharmacy , King Saud University , Riyadh , Saudi Arabia.b Department of Pharmacognosy, College of Pharmacy , King Saud University , Riyadh , Saudi Arabia.b Department of Pharmacognosy, College of Pharmacy , King Saud University , Riyadh , Saudi Arabia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28508699

Citation

Albadawi, Dina A., et al. "Antimicrobial, Anticancer, and Antioxidant Compounds From Premna Resinosa Growing in Saudi Arabia." Pharmaceutical Biology, vol. 55, no. 1, 2017, pp. 1759-1766.
Albadawi DA, Mothana RA, Khaled JM, et al. Antimicrobial, anticancer, and antioxidant compounds from Premna resinosa growing in Saudi Arabia. Pharm Biol. 2017;55(1):1759-1766.
Albadawi, D. A., Mothana, R. A., Khaled, J. M., Ashour, A. E., Kumar, A., Ahmad, S. F., Al-Said, M. S., Al-Rehaily, A. J., & Almusayeib, N. M. (2017). Antimicrobial, anticancer, and antioxidant compounds from Premna resinosa growing in Saudi Arabia. Pharmaceutical Biology, 55(1), 1759-1766. https://doi.org/10.1080/13880209.2017.1322617
Albadawi DA, et al. Antimicrobial, Anticancer, and Antioxidant Compounds From Premna Resinosa Growing in Saudi Arabia. Pharm Biol. 2017;55(1):1759-1766. PubMed PMID: 28508699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial, anticancer, and antioxidant compounds from Premna resinosa growing in Saudi Arabia. AU - Albadawi,Dina A, AU - Mothana,Ramzi A, AU - Khaled,Jamal M, AU - Ashour,Abdelkhader E, AU - Kumar,Ashok, AU - Ahmad,Sheikh F, AU - Al-Said,Mansour S, AU - Al-Rehaily,Adnan J, AU - Almusayeib,Nawal M, PY - 2017/5/17/entrez PY - 2017/5/17/pubmed PY - 2018/1/6/medline KW - Premna species KW - antibacterial KW - antioxidative KW - cytotoxic KW - flavonoids SP - 1759 EP - 1766 JF - Pharmaceutical biology JO - Pharm Biol VL - 55 IS - 1 N2 - CONTEXT: Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. OBJECTIVE: This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constituents. MATERIALS AND METHODS: The methanol extract of P. resinosa aerial parts and its fractions (n-hexane, dichloromethane, ethyl acetate and n-butanol) were tested. Antimicrobial activity was tested using microdilution method against three Gram-positive and four Gram-negative bacteria. The tested concentrations ranged from 4000 to 7.8 μg/mL and MIC values were determined after 24 h incubation. Anticancer activity was evaluated against three human cancer cell lines (Daoy, HepG2 and SK-MEL28) using MTT assay. Antioxidant activity was investigated by DPPH scavenging method and β-carotene-linoleic acid assay. RESULTS: The greatest antimicrobial activity was exhibited by n-hexane fraction (MIC 10 μg/mL) against Staphylococcus aureus, Enterococcus faecalis, and Shigella flexneri. The n-hexane fraction induced the greatest cytotoxic activity against Daoy, HepG2, and SK-MEL28 cell lines with IC50 values of 9.0, 8.5 and 13.2, respectively. Moreover, the dichloromethane and ethyl acetate fractions showed the highest antioxidant potential. A bioassay-guided fractionation led to the isolation and characterization of seven compounds for the first time, namely, quercetin (1), 3-methoxy quercetin (2), kaempferol (3), 3-methoxy kaempferol (4), myricetin 3,7,3'-trimethyl ether (5), lupeol (6), and stigmasterol (7). CONCLUSION: Our results indicate that P. resinosa is a source for antimicrobial and cytotoxic compounds. However, further work is required to isolate other active principles and to determine the mechanism of action. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/28508699/Antimicrobial_anticancer_and_antioxidant_compounds_from_Premna_resinosa_growing_in_Saudi_Arabia_ L2 - https://www.tandfonline.com/doi/full/10.1080/13880209.2017.1322617 DB - PRIME DP - Unbound Medicine ER -