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Enzyme-mediated formulation of stable elliptical silver nanoparticles tested against clinical pathogens and MDR bacteria and development of antimicrobial surgical thread.
Ann Clin Microbiol Antimicrob. 2017 May 16; 16(1):39.AC

Abstract

BACKGROUND

Silver nanoparticles (AgNPs) are believed to be emerging tool against various infectious diseases including multi-drug resistant (MDR) bacteria. In the present study, in vitro synthesis of AgNPs was optimized using 1:50 ratio of macerozyme (25 μg/μl) and 1 mM AgNO3 incubated at 80 °C for 8 h. AgNPs were characterized by UV-Visible spectroscopy, dynamic light scattering (DLS), scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy (TEM) and X-ray diffraction (XRD).

RESULTS

Characterization studies suggest the synthesis of elliptical, stable and crystalline AgNPs with an average size of 38.26 ± 0.4 nm calculated using TEM. The XRD pattern revealed the face-centered-cubic (fcc) form of metallic silver. Good shape integrity and dispersion of AgNPs after 1 year of incubation confirmed their stability. AgNPs were exibited the antimicrobial property against ten pathogenic bacteria, three molds and one yeast. The AgNPs also revealed remarkable antimicrobial activity against three MDR strains i.e. Extended spectrum beta-lactamase positive Escherichia coli, Staphylococcus aureus (MRSA) and Teicoplanin resistant Streptococcus Pneumoniae. The AgNPs coated surgical threads (suture) were revealed the remarkble antibacterial activity against three MDR strains. This is the first report to synthesize antimicrobial elliptical AgNPs using enzymes.

CONCLUSION

The results suggest the possibilities to develop the nanoparticles coated antimicrobial medical fabric to combat against MDR infection.

Authors+Show Affiliations

Department of Biotechnology, Veer Narmad South Gujarat University, Surat, Gujarat, 395007, India.Department of Biosciences (UGC-SAP-DRS-II), Veer Narmad South Gujarat University, Surat, Gujarat, 395007, India.Department of Biotechnology, Veer Narmad South Gujarat University, Surat, Gujarat, 395007, India.Department of Biotechnology, Veer Narmad South Gujarat University, Surat, Gujarat, 395007, India.Department of Biosciences (UGC-SAP-DRS-II), Veer Narmad South Gujarat University, Surat, Gujarat, 395007, India. dudhagarapr@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28511708

Citation

Thapa, Rupak, et al. "Enzyme-mediated Formulation of Stable Elliptical Silver Nanoparticles Tested Against Clinical Pathogens and MDR Bacteria and Development of Antimicrobial Surgical Thread." Annals of Clinical Microbiology and Antimicrobials, vol. 16, no. 1, 2017, p. 39.
Thapa R, Bhagat C, Shrestha P, et al. Enzyme-mediated formulation of stable elliptical silver nanoparticles tested against clinical pathogens and MDR bacteria and development of antimicrobial surgical thread. Ann Clin Microbiol Antimicrob. 2017;16(1):39.
Thapa, R., Bhagat, C., Shrestha, P., Awal, S., & Dudhagara, P. (2017). Enzyme-mediated formulation of stable elliptical silver nanoparticles tested against clinical pathogens and MDR bacteria and development of antimicrobial surgical thread. Annals of Clinical Microbiology and Antimicrobials, 16(1), 39. https://doi.org/10.1186/s12941-017-0216-y
Thapa R, et al. Enzyme-mediated Formulation of Stable Elliptical Silver Nanoparticles Tested Against Clinical Pathogens and MDR Bacteria and Development of Antimicrobial Surgical Thread. Ann Clin Microbiol Antimicrob. 2017 May 16;16(1):39. PubMed PMID: 28511708.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzyme-mediated formulation of stable elliptical silver nanoparticles tested against clinical pathogens and MDR bacteria and development of antimicrobial surgical thread. AU - Thapa,Rupak, AU - Bhagat,Chintan, AU - Shrestha,Pragya, AU - Awal,Suvash, AU - Dudhagara,Pravin, Y1 - 2017/05/16/ PY - 2017/4/4/received PY - 2017/5/12/accepted PY - 2017/5/18/entrez PY - 2017/5/18/pubmed PY - 2018/1/6/medline KW - Antimicrobial activity KW - Multi-drug resistant (MDR) KW - Silver nanoparticles (AgNPs) KW - Surgical thread SP - 39 EP - 39 JF - Annals of clinical microbiology and antimicrobials JO - Ann Clin Microbiol Antimicrob VL - 16 IS - 1 N2 - BACKGROUND: Silver nanoparticles (AgNPs) are believed to be emerging tool against various infectious diseases including multi-drug resistant (MDR) bacteria. In the present study, in vitro synthesis of AgNPs was optimized using 1:50 ratio of macerozyme (25 μg/μl) and 1 mM AgNO3 incubated at 80 °C for 8 h. AgNPs were characterized by UV-Visible spectroscopy, dynamic light scattering (DLS), scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy (TEM) and X-ray diffraction (XRD). RESULTS: Characterization studies suggest the synthesis of elliptical, stable and crystalline AgNPs with an average size of 38.26 ± 0.4 nm calculated using TEM. The XRD pattern revealed the face-centered-cubic (fcc) form of metallic silver. Good shape integrity and dispersion of AgNPs after 1 year of incubation confirmed their stability. AgNPs were exibited the antimicrobial property against ten pathogenic bacteria, three molds and one yeast. The AgNPs also revealed remarkable antimicrobial activity against three MDR strains i.e. Extended spectrum beta-lactamase positive Escherichia coli, Staphylococcus aureus (MRSA) and Teicoplanin resistant Streptococcus Pneumoniae. The AgNPs coated surgical threads (suture) were revealed the remarkble antibacterial activity against three MDR strains. This is the first report to synthesize antimicrobial elliptical AgNPs using enzymes. CONCLUSION: The results suggest the possibilities to develop the nanoparticles coated antimicrobial medical fabric to combat against MDR infection. SN - 1476-0711 UR - https://www.unboundmedicine.com/medline/citation/28511708/Enzyme_mediated_formulation_of_stable_elliptical_silver_nanoparticles_tested_against_clinical_pathogens_and_MDR_bacteria_and_development_of_antimicrobial_surgical_thread_ DB - PRIME DP - Unbound Medicine ER -