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Transcriptional and Physiological Responses to Nutrient Loading on Toxin Formation and Photosynthesis in Microcystis Aeruginosa FACHB-905.
Toxins (Basel). 2017 05 17; 9(5)T

Abstract

An important goal of understanding harmful algae blooms is to determine how environmental factors affect the growth and toxin formation of toxin-producing species. In this study, we investigated the transcriptional responses of toxin formation gene (mcyB) and key photosynthesis genes (psaB, psbD and rbcL) of Microcystis aeruginosa FACHB-905 in different nutrient loading conditions using real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). Three physio-biochemical parameters (malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH)) were also evaluated to provide insight into the physiological responses of Microcystis cells. We observed an upregulation of mcyB gene in nutrient-deficient conditions, especially in nitrogen (N) limitation condition, and the transcript abundance declined after the nutrient were resupplied. Differently, high transcription levels were seen in phosphorus (P) deficient treatments for key photosynthesis genes throughout the culture period, while those in N-deficient cells varied with time, suggesting an adaptive regulation of Microsystis cells to nutrient stress. Increased contents of antioxidant enzymes (SOD and GSH) were seen in both N and P-deficient conditions, suggesting the presence of excess amount of free radical generation caused by nutrient stress. The amount of SOD and GSH continued to increase even after the nutrient was reintroduced and a strong correlation was seen between the MDA and enzyme activities, indicating the robust effort of rebalancing the redox system in Microcystis cells. Based on these transcriptional and physiological responses of M. aeruginosa to nutrient loading, these results could provide more insight into Microcystis blooms management and toxin formation regulation.

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Authors+Show Affiliations

Peng G
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China. gtpeng11@fudan.edu.cn. College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China. gtpeng11@fudan.edu.cn.
Lin S
College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China. lin.sijie@tongji.edu.cn.
Fan Z
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China. zhqfan@fudan.edu.cn.
Wang X
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China. xrxrwang@fudan.edu.cn.

MeSH

Bacterial ProteinsGene Expression Regulation, BacterialGlutathioneHarmful Algal BloomMalondialdehydeMicrocystinsMicrocystisNitrogenPhosphorusPhotosynthesisPhotosystem I Protein ComplexRibulose-Bisphosphate CarboxylaseSuperoxide Dismutase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28513574

Citation

Peng, Guotao, et al. "Transcriptional and Physiological Responses to Nutrient Loading On Toxin Formation and Photosynthesis in Microcystis Aeruginosa FACHB-905." Toxins, vol. 9, no. 5, 2017.
Peng G, Lin S, Fan Z, et al. Transcriptional and Physiological Responses to Nutrient Loading on Toxin Formation and Photosynthesis in Microcystis Aeruginosa FACHB-905. Toxins (Basel). 2017;9(5).
Peng, G., Lin, S., Fan, Z., & Wang, X. (2017). Transcriptional and Physiological Responses to Nutrient Loading on Toxin Formation and Photosynthesis in Microcystis Aeruginosa FACHB-905. Toxins, 9(5). https://doi.org/10.3390/toxins9050168
Peng G, et al. Transcriptional and Physiological Responses to Nutrient Loading On Toxin Formation and Photosynthesis in Microcystis Aeruginosa FACHB-905. Toxins (Basel). 2017 05 17;9(5) PubMed PMID: 28513574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional and Physiological Responses to Nutrient Loading on Toxin Formation and Photosynthesis in Microcystis Aeruginosa FACHB-905. AU - Peng,Guotao, AU - Lin,Sijie, AU - Fan,Zhengqiu, AU - Wang,Xiangrong, Y1 - 2017/05/17/ PY - 2017/04/06/received PY - 2017/05/08/revised PY - 2017/05/11/accepted PY - 2017/5/18/entrez PY - 2017/5/18/pubmed PY - 2018/3/3/medline KW - antioxidant system KW - mcy KW - microcystin KW - nitrogen KW - phosphorus JF - Toxins JO - Toxins (Basel) VL - 9 IS - 5 N2 - An important goal of understanding harmful algae blooms is to determine how environmental factors affect the growth and toxin formation of toxin-producing species. In this study, we investigated the transcriptional responses of toxin formation gene (mcyB) and key photosynthesis genes (psaB, psbD and rbcL) of Microcystis aeruginosa FACHB-905 in different nutrient loading conditions using real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). Three physio-biochemical parameters (malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH)) were also evaluated to provide insight into the physiological responses of Microcystis cells. We observed an upregulation of mcyB gene in nutrient-deficient conditions, especially in nitrogen (N) limitation condition, and the transcript abundance declined after the nutrient were resupplied. Differently, high transcription levels were seen in phosphorus (P) deficient treatments for key photosynthesis genes throughout the culture period, while those in N-deficient cells varied with time, suggesting an adaptive regulation of Microsystis cells to nutrient stress. Increased contents of antioxidant enzymes (SOD and GSH) were seen in both N and P-deficient conditions, suggesting the presence of excess amount of free radical generation caused by nutrient stress. The amount of SOD and GSH continued to increase even after the nutrient was reintroduced and a strong correlation was seen between the MDA and enzyme activities, indicating the robust effort of rebalancing the redox system in Microcystis cells. Based on these transcriptional and physiological responses of M. aeruginosa to nutrient loading, these results could provide more insight into Microcystis blooms management and toxin formation regulation. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/28513574/Transcriptional_and_Physiological_Responses_to_Nutrient_Loading_on_Toxin_Formation_and_Photosynthesis_in_Microcystis_Aeruginosa_FACHB_905_ L2 - https://www.mdpi.com/resolver?pii=toxins9050168 DB - PRIME DP - Unbound Medicine ER -