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Two new tools: multi-purpose cloning vectors that carry kanamycin or spectinomycin/streptomycin resistance markers.
Gene 1988; 68(1):163-5GENE

Abstract

We have constructed two new multi-purpose cloning vectors, pJKKmf(-) and pJKSp/Smf(-), that carry resistance to kanamycin (Km) and spectinomycin/streptomycin (Sp/Sm), respectively. These plasmids are based on pGEM3Zf(-) and therefore contain a pUC-vector-derived multiple cloning site for 13 restriction sites flanked by T7 and SP6 RNA polymerase promoters, the alpha-peptide coding region of beta-galactosidase for blue/white colony screening and the bacteriophage f1 origin of replication for production of single-stranded DNA in the presence of a helper phage. We have used these vectors to reclone sequences from a maize genomic library, to produce radiolabeled RNA probes and to make single-stranded DNA.

Authors+Show Affiliations

Agrigenetics Advanced Science Company, Madison, WI 53716.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2851490

Citation

Kirschman, J A., and J H. Cramer. "Two New Tools: Multi-purpose Cloning Vectors That Carry Kanamycin or Spectinomycin/streptomycin Resistance Markers." Gene, vol. 68, no. 1, 1988, pp. 163-5.
Kirschman JA, Cramer JH. Two new tools: multi-purpose cloning vectors that carry kanamycin or spectinomycin/streptomycin resistance markers. Gene. 1988;68(1):163-5.
Kirschman, J. A., & Cramer, J. H. (1988). Two new tools: multi-purpose cloning vectors that carry kanamycin or spectinomycin/streptomycin resistance markers. Gene, 68(1), pp. 163-5.
Kirschman JA, Cramer JH. Two New Tools: Multi-purpose Cloning Vectors That Carry Kanamycin or Spectinomycin/streptomycin Resistance Markers. Gene. 1988 Aug 15;68(1):163-5. PubMed PMID: 2851490.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two new tools: multi-purpose cloning vectors that carry kanamycin or spectinomycin/streptomycin resistance markers. AU - Kirschman,J A, AU - Cramer,J H, PY - 1988/8/15/pubmed PY - 1988/8/15/medline PY - 1988/8/15/entrez SP - 163 EP - 5 JF - Gene JO - Gene VL - 68 IS - 1 N2 - We have constructed two new multi-purpose cloning vectors, pJKKmf(-) and pJKSp/Smf(-), that carry resistance to kanamycin (Km) and spectinomycin/streptomycin (Sp/Sm), respectively. These plasmids are based on pGEM3Zf(-) and therefore contain a pUC-vector-derived multiple cloning site for 13 restriction sites flanked by T7 and SP6 RNA polymerase promoters, the alpha-peptide coding region of beta-galactosidase for blue/white colony screening and the bacteriophage f1 origin of replication for production of single-stranded DNA in the presence of a helper phage. We have used these vectors to reclone sequences from a maize genomic library, to produce radiolabeled RNA probes and to make single-stranded DNA. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/2851490/Two_new_tools:_multi_purpose_cloning_vectors_that_carry_kanamycin_or_spectinomycin/streptomycin_resistance_markers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-1119(88)90609-9 DB - PRIME DP - Unbound Medicine ER -