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Sperm chromatin quality and DNA integrity in partial versus total globozoospermia.
Andrologia. 2018 Feb; 50(1)A

Abstract

Globozoospermia is a severe form of teratozoospermia with low incidence in infertile patients, considered as one of the important causes of male infertility. The objective was to investigate the chromatin/DNA integrity as well as apoptosis in ejaculated spermatozoa of cases with partial or total globozoospermia. Fifty-seven semen samples were divided into three groups of partial globozoospermia (n = 17), total globozoospermia (n = 10) and normozoospermia (control; n = 30). Sperm chromatin condensation, DNA integrity and apoptosis were assessed using cytochemical assays. The results showed significant differences in sperm parameters of count and motility between two case groups versus controls. The percentages of spermatozoa with abnormal chromatin packaging and protamine deficiency were significantly higher in total and partial globozoospermic men compared to normozoospermic samples. Also, the rates of TUNEL-positive spermatozoa were significantly increased in both globozoospermic cases with respect to the control (18.3 ± 10.1 and 12.3 ± 9.2 versus 5.9 ± 3 respectively). However, no significant differences were noticed between two subgroups of patients with regard to sperm DNA denaturation, DNA fragmentation and apoptosis. Abnormal chromatin packaging, DNA damage and apoptosis were significantly higher in cases than controls. The sperm chromatin/DNA anomalies may be considered as one of the main aetiology of ART failure in globozoospermic patients.

Authors+Show Affiliations

Recurrent Abortion Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Departments of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28517043

Citation

Talebi, A R., et al. "Sperm Chromatin Quality and DNA Integrity in Partial Versus Total Globozoospermia." Andrologia, vol. 50, no. 1, 2018.
Talebi AR, Ghasemzadeh J, Khalili MA, et al. Sperm chromatin quality and DNA integrity in partial versus total globozoospermia. Andrologia. 2018;50(1).
Talebi, A. R., Ghasemzadeh, J., Khalili, M. A., Halvaei, I., & Fesahat, F. (2018). Sperm chromatin quality and DNA integrity in partial versus total globozoospermia. Andrologia, 50(1). https://doi.org/10.1111/and.12823
Talebi AR, et al. Sperm Chromatin Quality and DNA Integrity in Partial Versus Total Globozoospermia. Andrologia. 2018;50(1) PubMed PMID: 28517043.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sperm chromatin quality and DNA integrity in partial versus total globozoospermia. AU - Talebi,A R, AU - Ghasemzadeh,J, AU - Khalili,M A, AU - Halvaei,I, AU - Fesahat,F, Y1 - 2017/05/18/ PY - 2017/02/07/accepted PY - 2017/5/19/pubmed PY - 2018/8/9/medline PY - 2017/5/19/entrez KW - DNA integrity KW - globozoospermia KW - male infertility KW - sperm chromatin JF - Andrologia JO - Andrologia VL - 50 IS - 1 N2 - Globozoospermia is a severe form of teratozoospermia with low incidence in infertile patients, considered as one of the important causes of male infertility. The objective was to investigate the chromatin/DNA integrity as well as apoptosis in ejaculated spermatozoa of cases with partial or total globozoospermia. Fifty-seven semen samples were divided into three groups of partial globozoospermia (n = 17), total globozoospermia (n = 10) and normozoospermia (control; n = 30). Sperm chromatin condensation, DNA integrity and apoptosis were assessed using cytochemical assays. The results showed significant differences in sperm parameters of count and motility between two case groups versus controls. The percentages of spermatozoa with abnormal chromatin packaging and protamine deficiency were significantly higher in total and partial globozoospermic men compared to normozoospermic samples. Also, the rates of TUNEL-positive spermatozoa were significantly increased in both globozoospermic cases with respect to the control (18.3 ± 10.1 and 12.3 ± 9.2 versus 5.9 ± 3 respectively). However, no significant differences were noticed between two subgroups of patients with regard to sperm DNA denaturation, DNA fragmentation and apoptosis. Abnormal chromatin packaging, DNA damage and apoptosis were significantly higher in cases than controls. The sperm chromatin/DNA anomalies may be considered as one of the main aetiology of ART failure in globozoospermic patients. SN - 1439-0272 UR - https://www.unboundmedicine.com/medline/citation/28517043/Sperm_chromatin_quality_and_DNA_integrity_in_partial_versus_total_globozoospermia_ L2 - https://doi.org/10.1111/and.12823 DB - PRIME DP - Unbound Medicine ER -