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A systematic genetic screen for genes involved in sensing inorganic phosphate availability in Saccharomyces cerevisiae.
PLoS One. 2017; 12(5):e0176085.Plos

Abstract

Saccharomyces cerevisiae responds to changes in extracellular inorganic phosphate (Pi) availability by regulating the activity of the phosphate-responsive (PHO) signaling pathway, enabling cells to maintain intracellular levels of the essential nutrient Pi. Pi-limitation induces upregulation of inositol heptakisphosphate (IP7) synthesized by the inositol hexakisphosphate kinase Vip1, triggering inhibition of the Pho80/Pho85 cyclin-cyclin dependent kinase (CDK) complex by the CDK inhibitor Pho81, which upregulates the PHO regulon through the CDK target and transcription factor Pho4. To identify genes that are involved in signaling upstream of the Pho80/Pho85/Pho81 complex and how they interact with each other to regulate the PHO pathway, we performed genome-wide screens with the synthetic genetic array method. We identified more than 300 mutants with defects in signaling upstream of the Pho80/Pho85/Pho81 complex, including AAH1, which encodes an adenine deaminase that negatively regulates the PHO pathway in a Vip1-dependent manner. Furthermore, we showed that even in the absence of VIP1, the PHO pathway can be activated under prolonged periods of Pi starvation, suggesting complexity in the mechanisms by which the PHO pathway is regulated.

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Authors+Show Affiliations

Choi J
Faculty of Arts and Sciences Center for Systems Biology, Harvard University, Cambridge, Massachusetts, United States of America. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America. Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, United States of America.
Rajagopal A
Faculty of Arts and Sciences Center for Systems Biology, Harvard University, Cambridge, Massachusetts, United States of America. Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, United States of America. Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States of America.
Xu YF
Department of Chemistry, Princeton University, Princeton, New Jersey, United States of America. Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America.
Rabinowitz JD
Department of Chemistry, Princeton University, Princeton, New Jersey, United States of America. Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America.
O'Shea EK
Faculty of Arts and Sciences Center for Systems Biology, Harvard University, Cambridge, Massachusetts, United States of America. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America. Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, United States of America. Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States of America.

MeSH

Gene Expression Regulation, FungalGenome, FungalPhosphatesSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28520786

Citation

Choi, Joonhyuk, et al. "A Systematic Genetic Screen for Genes Involved in Sensing Inorganic Phosphate Availability in Saccharomyces Cerevisiae." PloS One, vol. 12, no. 5, 2017, pp. e0176085.
Choi J, Rajagopal A, Xu YF, et al. A systematic genetic screen for genes involved in sensing inorganic phosphate availability in Saccharomyces cerevisiae. PLoS One. 2017;12(5):e0176085.
Choi, J., Rajagopal, A., Xu, Y. F., Rabinowitz, J. D., & O'Shea, E. K. (2017). A systematic genetic screen for genes involved in sensing inorganic phosphate availability in Saccharomyces cerevisiae. PloS One, 12(5), e0176085. https://doi.org/10.1371/journal.pone.0176085
Choi J, et al. A Systematic Genetic Screen for Genes Involved in Sensing Inorganic Phosphate Availability in Saccharomyces Cerevisiae. PLoS One. 2017;12(5):e0176085. PubMed PMID: 28520786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A systematic genetic screen for genes involved in sensing inorganic phosphate availability in Saccharomyces cerevisiae. AU - Choi,Joonhyuk, AU - Rajagopal,Abbhirami, AU - Xu,Yi-Fan, AU - Rabinowitz,Joshua D, AU - O'Shea,Erin K, Y1 - 2017/05/17/ PY - 2017/02/13/received PY - 2017/04/05/accepted PY - 2017/5/19/entrez PY - 2017/5/19/pubmed PY - 2017/9/7/medline SP - e0176085 EP - e0176085 JF - PloS one JO - PLoS One VL - 12 IS - 5 N2 - Saccharomyces cerevisiae responds to changes in extracellular inorganic phosphate (Pi) availability by regulating the activity of the phosphate-responsive (PHO) signaling pathway, enabling cells to maintain intracellular levels of the essential nutrient Pi. Pi-limitation induces upregulation of inositol heptakisphosphate (IP7) synthesized by the inositol hexakisphosphate kinase Vip1, triggering inhibition of the Pho80/Pho85 cyclin-cyclin dependent kinase (CDK) complex by the CDK inhibitor Pho81, which upregulates the PHO regulon through the CDK target and transcription factor Pho4. To identify genes that are involved in signaling upstream of the Pho80/Pho85/Pho81 complex and how they interact with each other to regulate the PHO pathway, we performed genome-wide screens with the synthetic genetic array method. We identified more than 300 mutants with defects in signaling upstream of the Pho80/Pho85/Pho81 complex, including AAH1, which encodes an adenine deaminase that negatively regulates the PHO pathway in a Vip1-dependent manner. Furthermore, we showed that even in the absence of VIP1, the PHO pathway can be activated under prolonged periods of Pi starvation, suggesting complexity in the mechanisms by which the PHO pathway is regulated. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28520786/A_systematic_genetic_screen_for_genes_involved_in_sensing_inorganic_phosphate_availability_in_Saccharomyces_cerevisiae_ L2 - https://dx.plos.org/10.1371/journal.pone.0176085 DB - PRIME DP - Unbound Medicine ER -