Tags

Type your tag names separated by a space and hit enter

In vitro and in vivo antioxidative and hepatoprotective activity of aqueous extract of Cortex Dictamni.
World J Gastroenterol. 2017 Apr 28; 23(16):2912-2927.WJ

Abstract

AIM

To investigate the antioxidant and hepatoprotective effects of Cortex Dictamni aqueous extract (CDAE) in carbon tetrachloride (CCl₄)-induced liver damage in rats.

METHODS

The in vitro antioxidant effect of CDAE was investigated using α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), β-carotene bleaching, reducing power, and thiobarbituric acid reactive substance assays. A linoleic acid system, including ferric thiocyanate (FTC) and thiobarbituric acid (TBA) assays, was used to evaluate the inhibition of lipid peroxidation. The in vivo hepatoprotective and antioxidant effects of CDAE against CCl₄-induced liver damage were evaluated in Sprague-Dawley rats. Silymarin was used as a positive control. Liver damage was assessed by determining hepatic histopathology and liver marker enzymes in serum. Enzyme and non-enzyme antioxidant levels and lipid peroxide content were measured in the liver. Cytochrome P450 2E1 (CYP2E1) protein expression was measured via immunohistochemical staining. Nuclear factor E2-related factor (Nrf2), heme oxygenase-1 (HO-1), NAD(P)H quinine oxidoreductase 1 (NQO1), and γ-glutamylcysteine synthetase catalytic subunit (γ-GCSc) protein expression was measured by Western blot.

RESULTS

Our results showed that CDAE exhibited a strong antioxidant activity in vitro. CDAE scavenged DPPH and ABTS radicals in a dose-dependent manner. CDAE inhibited lipid peroxidation with a lipid peroxide inhibition rate of 40.6% ± 5.2%. In the FTC and TBA assays, CDAE significantly inhibited lipid peroxidation (P < 0.01). In vivo histopathological studies indicated that CCl₄-induced liver injury was alleviated following CDAE treatment in rats of both sexes. CDAE (160 and 320 mg/kg) significantly prevented CCl₄-induced elevations of alkaline phosphatase, glutamate pyruvate transaminase, aspartate aminotransferase, and total bilirubin levels in rats of both sexes (P < 0.05, 0.01, or 0.001). Moreover, CDAE restored the decreased activities of hepatic antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, as well as non-enzyme antioxidant glutathione, which were induced by CCl₄ treatment. CDAE significantly suppressed the up-regulation of CYP2E1 and promoted Nrf2, HO-1, NQO1, and γ-GCSc protein expression.

CONCLUSION

CDAE exhibits good antioxidant performance in vitro, with marked radical-scavenging and anti-lipid peroxidation activities. CDAE is effective in preventing CCl₄-induced hepatic damage in rats of both sexes. The hepatoprotective activity of CDAE may be attributable to its antioxidant activity, which may involve Keap1-Nrf2-mediated antioxidant regulation.

Links

Authors+Show Affiliations

Lin Li, Yun-Feng Zhou, Yan-Lin Li, Li-Li Wang, Yang Xu, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

Lin Li, Yun-Feng Zhou, Yan-Lin Li, Li-Li Wang, Yang Xu, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

Lin Li, Yun-Feng Zhou, Yan-Lin Li, Li-Li Wang, Yang Xu, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

Lin Li, Yun-Feng Zhou, Yan-Lin Li, Li-Li Wang, Yang Xu, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

Lin Li, Yun-Feng Zhou, Yan-Lin Li, Li-Li Wang, Yang Xu, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

Lin Li, Yun-Feng Zhou, Yan-Lin Li, Li-Li Wang, Yang Xu, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

Pub Type(s)

Comparative Study

Journal Article

Language

eng

PubMed ID

28522909

Citation

Li, Lin, et al. "In Vitro and in Vivo Antioxidative and Hepatoprotective Activity of Aqueous Extract of Cortex Dictamni." World Journal of Gastroenterology, vol. 23, no. 16, 2017, pp. 2912-2927.

Li L, Zhou YF, Li YL, et al. In vitro and in vivo antioxidative and hepatoprotective activity of aqueous extract of Cortex Dictamni. World J Gastroenterol. 2017;23(16):2912-2927.

Li, L., Zhou, Y. F., Li, Y. L., Wang, L. L., Arai, H., & Xu, Y. (2017). In vitro and in vivo antioxidative and hepatoprotective activity of aqueous extract of Cortex Dictamni. World Journal of Gastroenterology, 23(16), 2912-2927. https://doi.org/10.3748/wjg.v23.i16.2912

Li L, et al. In Vitro and in Vivo Antioxidative and Hepatoprotective Activity of Aqueous Extract of Cortex Dictamni. World J Gastroenterol. 2017 Apr 28;23(16):2912-2927. PubMed PMID: 28522909.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - In vitro and in vivo antioxidative and hepatoprotective activity of aqueous extract of Cortex Dictamni. AU - Li,Lin, AU - Zhou,Yun-Feng, AU - Li,Yan-Lin, AU - Wang,Li-Li, AU - Arai,Hiderori, AU - Xu,Yang, PY - 2017/1/11/received PY - 2017/2/28/revised PY - 2017/3/15/accepted PY - 2017/5/20/entrez PY - 2017/5/20/pubmed PY - 2018/5/17/medline KW - Antioxidant activity KW - Carbon tetrachloride KW - Cortex Dictamni KW - Hepatoprotective KW - Nrf2 SP - 2912 EP - 2927 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 23 IS - 16 N2 - AIM: To investigate the antioxidant and hepatoprotective effects of Cortex Dictamni aqueous extract (CDAE) in carbon tetrachloride (CCl4)-induced liver damage in rats. METHODS: The in vitro antioxidant effect of CDAE was investigated using α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), β-carotene bleaching, reducing power, and thiobarbituric acid reactive substance assays. A linoleic acid system, including ferric thiocyanate (FTC) and thiobarbituric acid (TBA) assays, was used to evaluate the inhibition of lipid peroxidation. The in vivo hepatoprotective and antioxidant effects of CDAE against CCl4-induced liver damage were evaluated in Sprague-Dawley rats. Silymarin was used as a positive control. Liver damage was assessed by determining hepatic histopathology and liver marker enzymes in serum. Enzyme and non-enzyme antioxidant levels and lipid peroxide content were measured in the liver. Cytochrome P450 2E1 (CYP2E1) protein expression was measured via immunohistochemical staining. Nuclear factor E2-related factor (Nrf2), heme oxygenase-1 (HO-1), NAD(P)H quinine oxidoreductase 1 (NQO1), and γ-glutamylcysteine synthetase catalytic subunit (γ-GCSc) protein expression was measured by Western blot. RESULTS: Our results showed that CDAE exhibited a strong antioxidant activity in vitro. CDAE scavenged DPPH and ABTS radicals in a dose-dependent manner. CDAE inhibited lipid peroxidation with a lipid peroxide inhibition rate of 40.6% ± 5.2%. In the FTC and TBA assays, CDAE significantly inhibited lipid peroxidation (P < 0.01). In vivo histopathological studies indicated that CCl4-induced liver injury was alleviated following CDAE treatment in rats of both sexes. CDAE (160 and 320 mg/kg) significantly prevented CCl4-induced elevations of alkaline phosphatase, glutamate pyruvate transaminase, aspartate aminotransferase, and total bilirubin levels in rats of both sexes (P < 0.05, 0.01, or 0.001). Moreover, CDAE restored the decreased activities of hepatic antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, as well as non-enzyme antioxidant glutathione, which were induced by CCl4 treatment. CDAE significantly suppressed the up-regulation of CYP2E1 and promoted Nrf2, HO-1, NQO1, and γ-GCSc protein expression. CONCLUSION: CDAE exhibits good antioxidant performance in vitro, with marked radical-scavenging and anti-lipid peroxidation activities. CDAE is effective in preventing CCl4-induced hepatic damage in rats of both sexes. The hepatoprotective activity of CDAE may be attributable to its antioxidant activity, which may involve Keap1-Nrf2-mediated antioxidant regulation. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/28522909/In_vitro_and_in_vivo_antioxidative_and_hepatoprotective_activity_of_aqueous_extract_of_Cortex_Dictamni_ DB - PRIME DP - Unbound Medicine ER -