Tags

Type your tag names separated by a space and hit enter

One Year Genome Evolution of Lausannevirus in Allopatric versus Sympatric Conditions.
Genome Biol Evol. 2017 06 01; 9(6):1432-1449.GB

Abstract

Amoeba-resisting microorganisms raised a great interest during the last decade. Among them, some large DNA viruses present huge genomes up to 2.5 Mb long, exceeding the size of small bacterial genomes. The rate of genome evolution in terms of mutation, deletion, and gene acquisition in these genomes is yet unknown. Given the suspected high plasticity of viral genomes, the microevolution of the 346 kb genome of Lausannevirus, a member of Megavirales, was studied. Hence, Lausannevirus was co-cultured within the amoeba Acanthamoeba castellanii over one year. Despite a low number of mutations, the virus showed a genome reduction of 3.7% after 12 months. Lausannevirus genome evolution in sympatric conditions was investigated by its co-culture with Estrella lausannensis, an obligate intracellular bacterium, in the amoeba A. castellanii during one year. Cultures were split every 3 months. Genome sequencing revealed that in these conditions both, Lausannevirus and E. lausannensis, show stable genome, presenting no major rearrangement. In fact, after one year they acquired from 2 to 7 and from 4 to 10 mutations per culture for Lausannevirus and E. lausannensis, respectively. Interestingly, different mutations in the endonuclease encoding genes of Lausannevirus were observed in different subcultures, highlighting the importance of this gene product in the replication of Lausannevirus. Conversely, mutations in E. lausannensis were mainly located in a gene encoding for a phosphoenolpyruvate-protein phosphotransferase (PtsI), implicated in sugar metabolism. Moreover, in our conditions and with our analyses we detected no horizontal gene transfer during one year of co-culture.

Authors+Show Affiliations

Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, Lausanne University Hospital, and University of Lausanne, Switzerland.Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, Lausanne University Hospital, and University of Lausanne, Switzerland. SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland.Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, Lausanne University Hospital, and University of Lausanne, Switzerland.SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland. Department of Ecology and Evolution, Biophore, University of Lausanne, Switzerland.Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, Lausanne University Hospital, and University of Lausanne, Switzerland.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28525571

Citation

Mueller, Linda, et al. "One Year Genome Evolution of Lausannevirus in Allopatric Versus Sympatric Conditions." Genome Biology and Evolution, vol. 9, no. 6, 2017, pp. 1432-1449.
Mueller L, Bertelli C, Pillonel T, et al. One Year Genome Evolution of Lausannevirus in Allopatric versus Sympatric Conditions. Genome Biol Evol. 2017;9(6):1432-1449.
Mueller, L., Bertelli, C., Pillonel, T., Salamin, N., & Greub, G. (2017). One Year Genome Evolution of Lausannevirus in Allopatric versus Sympatric Conditions. Genome Biology and Evolution, 9(6), 1432-1449. https://doi.org/10.1093/gbe/evx074
Mueller L, et al. One Year Genome Evolution of Lausannevirus in Allopatric Versus Sympatric Conditions. Genome Biol Evol. 2017 06 1;9(6):1432-1449. PubMed PMID: 28525571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - One Year Genome Evolution of Lausannevirus in Allopatric versus Sympatric Conditions. AU - Mueller,Linda, AU - Bertelli,Claire, AU - Pillonel,Trestan, AU - Salamin,Nicolas, AU - Greub,Gilbert, PY - 2017/05/17/accepted PY - 2017/5/20/pubmed PY - 2017/11/2/medline PY - 2017/5/20/entrez KW - Chlamydiales KW - Lausannevirus KW - Marseilleviridae KW - Megavirales KW - experimental evolution KW - single nucleotide polymorphisms (SNPs) SP - 1432 EP - 1449 JF - Genome biology and evolution JO - Genome Biol Evol VL - 9 IS - 6 N2 - Amoeba-resisting microorganisms raised a great interest during the last decade. Among them, some large DNA viruses present huge genomes up to 2.5 Mb long, exceeding the size of small bacterial genomes. The rate of genome evolution in terms of mutation, deletion, and gene acquisition in these genomes is yet unknown. Given the suspected high plasticity of viral genomes, the microevolution of the 346 kb genome of Lausannevirus, a member of Megavirales, was studied. Hence, Lausannevirus was co-cultured within the amoeba Acanthamoeba castellanii over one year. Despite a low number of mutations, the virus showed a genome reduction of 3.7% after 12 months. Lausannevirus genome evolution in sympatric conditions was investigated by its co-culture with Estrella lausannensis, an obligate intracellular bacterium, in the amoeba A. castellanii during one year. Cultures were split every 3 months. Genome sequencing revealed that in these conditions both, Lausannevirus and E. lausannensis, show stable genome, presenting no major rearrangement. In fact, after one year they acquired from 2 to 7 and from 4 to 10 mutations per culture for Lausannevirus and E. lausannensis, respectively. Interestingly, different mutations in the endonuclease encoding genes of Lausannevirus were observed in different subcultures, highlighting the importance of this gene product in the replication of Lausannevirus. Conversely, mutations in E. lausannensis were mainly located in a gene encoding for a phosphoenolpyruvate-protein phosphotransferase (PtsI), implicated in sugar metabolism. Moreover, in our conditions and with our analyses we detected no horizontal gene transfer during one year of co-culture. SN - 1759-6653 UR - https://www.unboundmedicine.com/medline/citation/28525571/One_Year_Genome_Evolution_of_Lausannevirus_in_Allopatric_versus_Sympatric_Conditions_ L2 - https://academic.oup.com/gbe/article-lookup/doi/10.1093/gbe/evx074 DB - PRIME DP - Unbound Medicine ER -