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Highly-sensitive aptasensor based on fluorescence resonance energy transfer between l-cysteine capped ZnS quantum dots and graphene oxide sheets for the determination of edifenphos fungicide.
Biosens Bioelectron. 2017 Oct 15; 96:324-331.BB

Abstract

With the advantages of excellent optical properties and biocompatibility, single-strand DNA-functionalized quantum dots have been widely applied in biosensing and bioimaging. A new aptasensor with easy operation, high sensitivity, and high selectivity was developed by immobilizing the aptamer on water soluble l-cysteine capped ZnS quantum dots (QDs). Graphene oxide (GO) sheets are mixed with the aptamer-QDs. Consequently, the aptamer-conjugated QDs bind to the GO sheets to form a GO/aptamer-QDs ensemble. This aptasensor enables the energy transfer based on a fluorescence resonance energy transfer (FRET) from the QDs to the GO sheets, quenching the fluorescence of QDs. The GO/aptamer-QDs ensemble assay acts as a "turn-on'' fluorescent sensor for edifenphos (EDI) detection. When GO was replaced by EDI, the fluorescence of QDs was restored and its intensity was proportional to the EDI concentration. This GO-based aptasensor under the optimum conditions exhibited excellent analytical performance for EDI determination, ranging from 5×10-4 to 6×10-3mg L-1 with the detection limit of 1.3×10-4mgL-1. Furthermore, the designed aptasensor exhibited excellent selectivity toward EDI compared to other pesticides and herbicides with similar structures such as diazinon, heptachlor, endrin, dieldrin, butachlor and chlordane. Good reproducibility and precision (RSD =3.9%, n =10) of the assay indicates the high potential of the aptasensor for quantitative trace analysis of EDI. Moreover, the results demonstrate the applicability of the aptasensor for monitoring EDI fungicide in spiked real samples.

Authors+Show Affiliations

Analytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box 1914, Rasht, Iran. Electronic address: arvand@guilan.ac.ir.Analytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box 1914, Rasht, Iran.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

28525850

Citation

Arvand, Majid, and Aazam A. Mirroshandel. "Highly-sensitive Aptasensor Based On Fluorescence Resonance Energy Transfer Between L-cysteine Capped ZnS Quantum Dots and Graphene Oxide Sheets for the Determination of Edifenphos Fungicide." Biosensors & Bioelectronics, vol. 96, 2017, pp. 324-331.
Arvand M, Mirroshandel AA. Highly-sensitive aptasensor based on fluorescence resonance energy transfer between l-cysteine capped ZnS quantum dots and graphene oxide sheets for the determination of edifenphos fungicide. Biosens Bioelectron. 2017;96:324-331.
Arvand, M., & Mirroshandel, A. A. (2017). Highly-sensitive aptasensor based on fluorescence resonance energy transfer between l-cysteine capped ZnS quantum dots and graphene oxide sheets for the determination of edifenphos fungicide. Biosensors & Bioelectronics, 96, 324-331. https://doi.org/10.1016/j.bios.2017.05.028
Arvand M, Mirroshandel AA. Highly-sensitive Aptasensor Based On Fluorescence Resonance Energy Transfer Between L-cysteine Capped ZnS Quantum Dots and Graphene Oxide Sheets for the Determination of Edifenphos Fungicide. Biosens Bioelectron. 2017 Oct 15;96:324-331. PubMed PMID: 28525850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly-sensitive aptasensor based on fluorescence resonance energy transfer between l-cysteine capped ZnS quantum dots and graphene oxide sheets for the determination of edifenphos fungicide. AU - Arvand,Majid, AU - Mirroshandel,Aazam A, Y1 - 2017/05/12/ PY - 2017/02/07/received PY - 2017/05/11/revised PY - 2017/05/12/accepted PY - 2017/5/20/pubmed PY - 2018/3/13/medline PY - 2017/5/20/entrez KW - Aptasensor KW - Edifenphos KW - Fluorescence resonance energy transfer KW - Graphene oxide KW - Quantum dots SP - 324 EP - 331 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 96 N2 - With the advantages of excellent optical properties and biocompatibility, single-strand DNA-functionalized quantum dots have been widely applied in biosensing and bioimaging. A new aptasensor with easy operation, high sensitivity, and high selectivity was developed by immobilizing the aptamer on water soluble l-cysteine capped ZnS quantum dots (QDs). Graphene oxide (GO) sheets are mixed with the aptamer-QDs. Consequently, the aptamer-conjugated QDs bind to the GO sheets to form a GO/aptamer-QDs ensemble. This aptasensor enables the energy transfer based on a fluorescence resonance energy transfer (FRET) from the QDs to the GO sheets, quenching the fluorescence of QDs. The GO/aptamer-QDs ensemble assay acts as a "turn-on'' fluorescent sensor for edifenphos (EDI) detection. When GO was replaced by EDI, the fluorescence of QDs was restored and its intensity was proportional to the EDI concentration. This GO-based aptasensor under the optimum conditions exhibited excellent analytical performance for EDI determination, ranging from 5×10-4 to 6×10-3mg L-1 with the detection limit of 1.3×10-4mgL-1. Furthermore, the designed aptasensor exhibited excellent selectivity toward EDI compared to other pesticides and herbicides with similar structures such as diazinon, heptachlor, endrin, dieldrin, butachlor and chlordane. Good reproducibility and precision (RSD =3.9%, n =10) of the assay indicates the high potential of the aptasensor for quantitative trace analysis of EDI. Moreover, the results demonstrate the applicability of the aptasensor for monitoring EDI fungicide in spiked real samples. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/28525850/Highly_sensitive_aptasensor_based_on_fluorescence_resonance_energy_transfer_between_l_cysteine_capped_ZnS_quantum_dots_and_graphene_oxide_sheets_for_the_determination_of_edifenphos_fungicide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(17)30338-X DB - PRIME DP - Unbound Medicine ER -