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Characterisation of Pomegranate-Husk Polyphenols and Semi-Preparative Fractionation of Punicalagin.
Phytochem Anal. 2017 Sep; 28(5):433-438.PA

Abstract

INTRODUCTION

Pomegranate-husk is the main by-product generated from the pomegranate industry. It is a potential source of compounds highly appreciated by different costumers. Punicalagin is the main compound present in pomegranate-husk.

OBJECTIVE

To characterise the pomegranate-husk total polyphenols by HPLC-ESI-MS and to establish a method for the recovery of punicalagin using a medium pressure liquid chromatography (MPLC) system.

MATERIALS AND METHODS

The characterisation of total pomegranate-husk polyphenols was carried out using liquid chromatography coupled to mass spectrometry. Thus, 200 mg of pomegranate-husk polyphenols were fractionated by MPLC. The isolated punicalagin was characterised by HPLC-MS and was tested as standard reagent for the measurement of its scavenging capacity reducing DPPH and ABTS radicals.

RESULTS

Twenty peaks were identified by analytical HPLC-MS analysis from the pomegranate-husk polyphenols. The main compounds were the punicalagin anomers, punicalin and ellagic acid. The MPLC method allowed three fractions to be obtained. In fraction three 39.40 ± 8.06 mg of punicalagin anomers (purity > 97.9%) were recovered. The scavenging capacity of punicalagin showed an IC50 of 109.53 and 151.50 μg/mL for DPPH and ABTS radicals, respectively.

CONCLUSION

The MPLC system was an excellent tool for the separation of the main ellagitannins from pomegranate husk and for the isolation of punicalagin anomers. Fraction three was rich in high purity punicalagin anomers. The IC50 was obtained for DPPH and ABTS radicals. Copyright © 2017 John Wiley & Sons, Ltd.

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Authors+Show Affiliations

Aguilar-Zárate P

Food Research Department, School of Chemistry, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, México.

Food Research Department, School of Chemistry, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, México.

Food Research Department, School of Chemistry, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, México.

Buenrostro-Figueroa J

Food Research Department, School of Chemistry, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, México.

Food Research Department, School of Chemistry, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, México.

Food Research Department, School of Chemistry, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, México.

Contreras-Esquivel JC

Food Research Department, School of Chemistry, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, México.

Gutiérrez-Sánchez G

Complex Carbohydrate Research Centre, The University of Georgia, Athens, GA, 30602, USA.

Food Research Department, School of Chemistry, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, México.

MeSH

Chromatography, High Pressure LiquidEllagic AcidHydrolyzable TanninsLythraceaeMass SpectrometryPolyphenols

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28543801

Citation

Aguilar-Zárate, Pedro, et al. "Characterisation of Pomegranate-Husk Polyphenols and Semi-Preparative Fractionation of Punicalagin." Phytochemical Analysis : PCA, vol. 28, no. 5, 2017, pp. 433-438.

Aguilar-Zárate P, Wong-Paz JE, Michel M, et al. Characterisation of Pomegranate-Husk Polyphenols and Semi-Preparative Fractionation of Punicalagin. Phytochem Anal. 2017;28(5):433-438.

Aguilar-Zárate, P., Wong-Paz, J. E., Michel, M., Buenrostro-Figueroa, J., Díaz, H. R., Ascacio, J. A., Contreras-Esquivel, J. C., Gutiérrez-Sánchez, G., & Aguilar, C. N. (2017). Characterisation of Pomegranate-Husk Polyphenols and Semi-Preparative Fractionation of Punicalagin. Phytochemical Analysis : PCA, 28(5), 433-438. https://doi.org/10.1002/pca.2691

Aguilar-Zárate P, et al. Characterisation of Pomegranate-Husk Polyphenols and Semi-Preparative Fractionation of Punicalagin. Phytochem Anal. 2017;28(5):433-438. PubMed PMID: 28543801.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Characterisation of Pomegranate-Husk Polyphenols and Semi-Preparative Fractionation of Punicalagin. AU - Aguilar-Zárate,Pedro, AU - Wong-Paz,Jorge E, AU - Michel,Mariela, AU - Buenrostro-Figueroa,Juan, AU - Díaz,Hugo R, AU - Ascacio,Juan A, AU - Contreras-Esquivel,Juan C, AU - Gutiérrez-Sánchez,Gerardo, AU - Aguilar,Cristóbal N, Y1 - 2017/05/23/ PY - 2017/02/05/received PY - 2017/03/20/revised PY - 2017/03/21/accepted PY - 2017/5/26/pubmed PY - 2017/12/30/medline PY - 2017/5/26/entrez KW - Analytical HPLC KW - MPLC KW - ellagitannins KW - mass spectrometry KW - polyphenols KW - punicalagin KW - punicalin SP - 433 EP - 438 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 28 IS - 5 N2 - INTRODUCTION: Pomegranate-husk is the main by-product generated from the pomegranate industry. It is a potential source of compounds highly appreciated by different costumers. Punicalagin is the main compound present in pomegranate-husk. OBJECTIVE: To characterise the pomegranate-husk total polyphenols by HPLC-ESI-MS and to establish a method for the recovery of punicalagin using a medium pressure liquid chromatography (MPLC) system. MATERIALS AND METHODS: The characterisation of total pomegranate-husk polyphenols was carried out using liquid chromatography coupled to mass spectrometry. Thus, 200 mg of pomegranate-husk polyphenols were fractionated by MPLC. The isolated punicalagin was characterised by HPLC-MS and was tested as standard reagent for the measurement of its scavenging capacity reducing DPPH and ABTS radicals. RESULTS: Twenty peaks were identified by analytical HPLC-MS analysis from the pomegranate-husk polyphenols. The main compounds were the punicalagin anomers, punicalin and ellagic acid. The MPLC method allowed three fractions to be obtained. In fraction three 39.40 ± 8.06 mg of punicalagin anomers (purity > 97.9%) were recovered. The scavenging capacity of punicalagin showed an IC50 of 109.53 and 151.50 μg/mL for DPPH and ABTS radicals, respectively. CONCLUSION: The MPLC system was an excellent tool for the separation of the main ellagitannins from pomegranate husk and for the isolation of punicalagin anomers. Fraction three was rich in high purity punicalagin anomers. The IC50 was obtained for DPPH and ABTS radicals. Copyright © 2017 John Wiley & Sons, Ltd. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/28543801/Characterisation_of_Pomegranate_Husk_Polyphenols_and_Semi_Preparative_Fractionation_of_Punicalagin_ DB - PRIME DP - Unbound Medicine ER -