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Molecular cloning, expression and insulin reduction activity of a thioredoxin 1 homologue (TRX1) from the pathogenic fungus Paracoccidioides lutzii.
Int J Biol Macromol. 2017 Oct; 103:683-691.IJ

Abstract

The dimorphic fungi Paracoccidioides spp. are the etiological agents of paracoccidioidomycosis (PCM), a prevalent systemic mycosis in Latin America. The Paracoccidioides lutzii response to oxidative stress is largely unexplored. Thioredoxins (TRX) are involved in the regulation of the redox environment in the cell, responding to oxidative stress in several organisms. In this study, we describe the isolation and characterization of a cDNA encoding a thioredoxin 1 from yeast cells from P. lutzii. The cDNA codes for a 12kDa protein containing the characteristic thioredoxin active site. The thioredoxin 1 gene was expressed in Escherichia coli and the isolated thioredoxin 1 recombinant protein as the native PlTRX1 from yeast cells showed insulin reduction activity in vitro. We also showed by semi-quantitative RT-PCR analysis that the expression of thioredoxin 1 gene was induced in response to H2O2 and may exert an antioxidant activity in vivo. Our results suggest that the thioredoxin 1 may play an important role in controlling the redox status in P. lutzii which may contribute to this organism's virulence.

Authors+Show Affiliations

Institute of Biological Sciences, Department of Biochemistry and Molecular Biology, Federal University of Goiás, 74001-970 Goiânia, Goiás, Brazil; School of Veterinary and Animal Science, Animal Pathology, Federal University of Goiás, 74001-970 Goiânia, Goiás, Brazil. Electronic address: lorenaccintra@gmail.com.Institute of Biological Sciences, Department of Biochemistry and Molecular Biology, Federal University of Goiás, 74001-970 Goiânia, Goiás, Brazil.Institute of Biological Sciences, Department of Biochemistry and Molecular Biology, Federal University of Goiás, 74001-970 Goiânia, Goiás, Brazil.Institute of Tropical Pathology and Public Health, Department of Microbiology, Immunology, Parasitology and Pathology, Federal University of Goiás, 74605-050 Goiânia, Goiás, Brazil.Institute of Biological Sciences, Department of Biochemistry and Molecular Biology, Federal University of Goiás, 74001-970 Goiânia, Goiás, Brazil.Institute of Biological Sciences, Department of Biochemistry and Molecular Biology, Federal University of Goiás, 74001-970 Goiânia, Goiás, Brazil.Institute of Biological Sciences, Department of Biochemistry and Molecular Biology, Federal University of Goiás, 74001-970 Goiânia, Goiás, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28545968

Citation

Cintra, Lorena C., et al. "Molecular Cloning, Expression and Insulin Reduction Activity of a Thioredoxin 1 Homologue (TRX1) From the Pathogenic Fungus Paracoccidioides Lutzii." International Journal of Biological Macromolecules, vol. 103, 2017, pp. 683-691.
Cintra LC, Domingos FC, Lima YAR, et al. Molecular cloning, expression and insulin reduction activity of a thioredoxin 1 homologue (TRX1) from the pathogenic fungus Paracoccidioides lutzii. Int J Biol Macromol. 2017;103:683-691.
Cintra, L. C., Domingos, F. C., Lima, Y. A. R., Barbosa, M. S., Santos, R. S., Faria, F. P., & Jesuíno, R. S. A. (2017). Molecular cloning, expression and insulin reduction activity of a thioredoxin 1 homologue (TRX1) from the pathogenic fungus Paracoccidioides lutzii. International Journal of Biological Macromolecules, 103, 683-691. https://doi.org/10.1016/j.ijbiomac.2017.05.114
Cintra LC, et al. Molecular Cloning, Expression and Insulin Reduction Activity of a Thioredoxin 1 Homologue (TRX1) From the Pathogenic Fungus Paracoccidioides Lutzii. Int J Biol Macromol. 2017;103:683-691. PubMed PMID: 28545968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning, expression and insulin reduction activity of a thioredoxin 1 homologue (TRX1) from the pathogenic fungus Paracoccidioides lutzii. AU - Cintra,Lorena C, AU - Domingos,Fernanda C, AU - Lima,Yanna A R, AU - Barbosa,Mônica S, AU - Santos,Rodrigo S, AU - Faria,Fabrícia P, AU - Jesuíno,Rosália S A, Y1 - 2017/05/22/ PY - 2017/05/08/received PY - 2017/05/17/revised PY - 2017/05/18/accepted PY - 2017/5/27/pubmed PY - 2018/3/21/medline PY - 2017/5/27/entrez KW - Oxidative stress KW - Paracoccidioides lutzii KW - Thioredoxin SP - 683 EP - 691 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 103 N2 - The dimorphic fungi Paracoccidioides spp. are the etiological agents of paracoccidioidomycosis (PCM), a prevalent systemic mycosis in Latin America. The Paracoccidioides lutzii response to oxidative stress is largely unexplored. Thioredoxins (TRX) are involved in the regulation of the redox environment in the cell, responding to oxidative stress in several organisms. In this study, we describe the isolation and characterization of a cDNA encoding a thioredoxin 1 from yeast cells from P. lutzii. The cDNA codes for a 12kDa protein containing the characteristic thioredoxin active site. The thioredoxin 1 gene was expressed in Escherichia coli and the isolated thioredoxin 1 recombinant protein as the native PlTRX1 from yeast cells showed insulin reduction activity in vitro. We also showed by semi-quantitative RT-PCR analysis that the expression of thioredoxin 1 gene was induced in response to H2O2 and may exert an antioxidant activity in vivo. Our results suggest that the thioredoxin 1 may play an important role in controlling the redox status in P. lutzii which may contribute to this organism's virulence. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/28545968/Molecular_cloning_expression_and_insulin_reduction_activity_of_a_thioredoxin_1_homologue__TRX1__from_the_pathogenic_fungus_Paracoccidioides_lutzii_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)31674-4 DB - PRIME DP - Unbound Medicine ER -