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Hepatoprotective efficacy of Spirulina platensis against lead-induced oxidative stress and genotoxicity in catfish; Clarias gariepinus.
Ecotoxicol Environ Saf. 2017 Sep; 143:344-350.EE

Abstract

Lead (Pb) is a toxic environmental pollutant that induces a broad range of biochemical and physiological hazards in living organisms. We investigated the possible hepatoprotective effects of Spirulina platensis (SP) in counteracting the Pb-induced oxidative damage. Ninety-six adult African catfish were allocated into four equal groups. The 1st group (control) fed basal diet while the 2nd group (Pb-treated) fed on basal diet and exposed to 1mg Pb(NO3)2/L. The 3rd and 4th groups fed SP-supplemented basal diets at levels of 0.25% and 0.5%, respectively and exposed to Pb. Serum samples were used to analyze hepatic function biomarkers, electrolytes, and oxidant and antioxidant status. Lipid peroxidation and DNA fragmentation were determined in the liver tissues. Pb exposure induced hepatic dysfunction, electrolytes (Na+, K+, Ca+2, and Cl-) imbalance, as well a significant decrease in GSH content, and LDH, AChE, SOD, CAT and GST enzymes activity. SP supplementation reverted these biochemical and genetic alterations close to control levels. This amelioration was higher with 0.5% SP and at the 4th week of exposure, showing concentration- and time-dependency. Thus, the current study suggests that SP could protect the catfish liver against lead-induced injury by scavenging ROS, sustaining the antioxidant status and diminishing DNA oxidative damage. The dietary inclusion of SP can be used as a promising protective agent to counteract oxidative stress-mediated diseases and toxicities.

Authors+Show Affiliations

Department of Zoology, Faculty of Science, Assiut University, Assiut 71516, Egypt. Electronic address: alaasayed@aun.edu.eg.Department of Veterinary Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28554489

Citation

Sayed, Alaa El-Din H., et al. "Hepatoprotective Efficacy of Spirulina Platensis Against Lead-induced Oxidative Stress and Genotoxicity in Catfish; Clarias Gariepinus." Ecotoxicology and Environmental Safety, vol. 143, 2017, pp. 344-350.
Sayed AEH, El-Sayed YS, El-Far AH. Hepatoprotective efficacy of Spirulina platensis against lead-induced oxidative stress and genotoxicity in catfish; Clarias gariepinus. Ecotoxicol Environ Saf. 2017;143:344-350.
Sayed, A. E. H., El-Sayed, Y. S., & El-Far, A. H. (2017). Hepatoprotective efficacy of Spirulina platensis against lead-induced oxidative stress and genotoxicity in catfish; Clarias gariepinus. Ecotoxicology and Environmental Safety, 143, 344-350. https://doi.org/10.1016/j.ecoenv.2017.05.013
Sayed AEH, El-Sayed YS, El-Far AH. Hepatoprotective Efficacy of Spirulina Platensis Against Lead-induced Oxidative Stress and Genotoxicity in Catfish; Clarias Gariepinus. Ecotoxicol Environ Saf. 2017;143:344-350. PubMed PMID: 28554489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective efficacy of Spirulina platensis against lead-induced oxidative stress and genotoxicity in catfish; Clarias gariepinus. AU - Sayed,Alaa El-Din H, AU - El-Sayed,Yasser S, AU - El-Far,Ali H, Y1 - 2017/05/27/ PY - 2017/03/16/received PY - 2017/05/01/revised PY - 2017/05/08/accepted PY - 2017/5/31/pubmed PY - 2017/9/29/medline PY - 2017/5/31/entrez KW - DNA fragmentation KW - Electrolytes KW - Hepatotoxicity KW - Lead KW - Oxidative stress KW - Spirulina platensis SP - 344 EP - 350 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 143 N2 - Lead (Pb) is a toxic environmental pollutant that induces a broad range of biochemical and physiological hazards in living organisms. We investigated the possible hepatoprotective effects of Spirulina platensis (SP) in counteracting the Pb-induced oxidative damage. Ninety-six adult African catfish were allocated into four equal groups. The 1st group (control) fed basal diet while the 2nd group (Pb-treated) fed on basal diet and exposed to 1mg Pb(NO3)2/L. The 3rd and 4th groups fed SP-supplemented basal diets at levels of 0.25% and 0.5%, respectively and exposed to Pb. Serum samples were used to analyze hepatic function biomarkers, electrolytes, and oxidant and antioxidant status. Lipid peroxidation and DNA fragmentation were determined in the liver tissues. Pb exposure induced hepatic dysfunction, electrolytes (Na+, K+, Ca+2, and Cl-) imbalance, as well a significant decrease in GSH content, and LDH, AChE, SOD, CAT and GST enzymes activity. SP supplementation reverted these biochemical and genetic alterations close to control levels. This amelioration was higher with 0.5% SP and at the 4th week of exposure, showing concentration- and time-dependency. Thus, the current study suggests that SP could protect the catfish liver against lead-induced injury by scavenging ROS, sustaining the antioxidant status and diminishing DNA oxidative damage. The dietary inclusion of SP can be used as a promising protective agent to counteract oxidative stress-mediated diseases and toxicities. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/28554489/Hepatoprotective_efficacy_of_Spirulina_platensis_against_lead_induced_oxidative_stress_and_genotoxicity_in_catfish L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(17)30282-8 DB - PRIME DP - Unbound Medicine ER -