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Inhibition of biofilm formation in Mycobacterium smegmatis by Parinari curatellifolia leaf extracts.
BMC Complement Altern Med. 2017 May 30; 17(1):285.BC

Abstract

BACKGROUND

Tuberculosis (TB) is a serious public health problem worldwide. Mycobacterium tuberculosis (M. tuberculosis) grows as drug tolerant pellicles. Agents that inhibit biofilm formation in M. tuberculosis have the potential to reduce the disease treatment period and improve the quality of tuberculosis chemotherapy. Parinari curatellifolia (P. curatellifolia) leaf extracts are claimed to treat symptoms similar to tuberculosis in ethnomedicinal practices. Mycobacterium smegmatis (M. smegmatis) is a surrogate organism used in antimycobacterial drug discovery assays. In this study, the effect of the leaf extracts of P. curatellifolia on M. smegmatis growth and biofilm formation was investigated in order to determine the basis of its use in traditional medicinal use.

METHODS

Phytochemicals from P. curatellifolia leaves were prepared using a mixture of 50% dichloromethane (DCM): 50% methanol and by serial exhaustive extraction using different solvents of decreasing polarity. The solvents were used in the following order, hexane > dichloromethane > ethyl acetate > acetone >ethanol > methanol > water. The micro-broth dilution method was used as an antimycobacterial susceptibility test to screen for the extract that effectively inhibited M. smegmatis growth and biofilm formation. Biofilm quantification was performed by staining the biofilms with crystal violet and determining the amount of the stain using a spectrophotometer. In addition, the effects of combining the most active extract with kanamycin were also investigated.

RESULTS

The minimum inhibitory concentrations (MIC) of the extracts were found to be 6.2 μg/ml for the acetone extract, 12.5 μg/ml for both the ethanol and the total extract and 50 μg/ml for both the methanol and ethyl acetate extracts. The ethanol extract, dichloromethane extract and water extract were the only extracts that effectively inhibited biofilm formation in M. smegmatis. Combining the ethanol extract with kanamycin enhanced the effect of the ethanol extract in terms of inhibition of biofilm formation.

CONCLUSIONS

P. curatellifolia leaves contain phytochemicals that have the potential to be used both as antimycobacterial and anti-biofilm formation compounds.

Authors+Show Affiliations

Bio-molecular Interactions Analyses Group, Department of Biochemistry, University of Zimbabwe, P.O. Box MP 167, Mt. Pleasant, Harare, Zimbabwe.Bio-molecular Interactions Analyses Group, Department of Biochemistry, University of Zimbabwe, P.O. Box MP 167, Mt. Pleasant, Harare, Zimbabwe.Bio-molecular Interactions Analyses Group, Department of Biochemistry, University of Zimbabwe, P.O. Box MP 167, Mt. Pleasant, Harare, Zimbabwe. smukanganyama@medic.uz.ac.zw.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28558683

Citation

Bhunu, Benjamin, et al. "Inhibition of Biofilm Formation in Mycobacterium Smegmatis By Parinari Curatellifolia Leaf Extracts." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 285.
Bhunu B, Mautsa R, Mukanganyama S. Inhibition of biofilm formation in Mycobacterium smegmatis by Parinari curatellifolia leaf extracts. BMC Complement Altern Med. 2017;17(1):285.
Bhunu, B., Mautsa, R., & Mukanganyama, S. (2017). Inhibition of biofilm formation in Mycobacterium smegmatis by Parinari curatellifolia leaf extracts. BMC Complementary and Alternative Medicine, 17(1), 285. https://doi.org/10.1186/s12906-017-1801-5
Bhunu B, Mautsa R, Mukanganyama S. Inhibition of Biofilm Formation in Mycobacterium Smegmatis By Parinari Curatellifolia Leaf Extracts. BMC Complement Altern Med. 2017 May 30;17(1):285. PubMed PMID: 28558683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of biofilm formation in Mycobacterium smegmatis by Parinari curatellifolia leaf extracts. AU - Bhunu,Benjamin, AU - Mautsa,Ruvimbo, AU - Mukanganyama,Stanley, Y1 - 2017/05/30/ PY - 2016/12/15/received PY - 2017/05/19/accepted PY - 2017/6/1/entrez PY - 2017/6/1/pubmed PY - 2017/8/22/medline KW - Biofilm formation KW - Isoniazid KW - Kanamycin KW - Mycobacterium smegmatis KW - Parinari curatellifolia KW - Tuberculosis SP - 285 EP - 285 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Tuberculosis (TB) is a serious public health problem worldwide. Mycobacterium tuberculosis (M. tuberculosis) grows as drug tolerant pellicles. Agents that inhibit biofilm formation in M. tuberculosis have the potential to reduce the disease treatment period and improve the quality of tuberculosis chemotherapy. Parinari curatellifolia (P. curatellifolia) leaf extracts are claimed to treat symptoms similar to tuberculosis in ethnomedicinal practices. Mycobacterium smegmatis (M. smegmatis) is a surrogate organism used in antimycobacterial drug discovery assays. In this study, the effect of the leaf extracts of P. curatellifolia on M. smegmatis growth and biofilm formation was investigated in order to determine the basis of its use in traditional medicinal use. METHODS: Phytochemicals from P. curatellifolia leaves were prepared using a mixture of 50% dichloromethane (DCM): 50% methanol and by serial exhaustive extraction using different solvents of decreasing polarity. The solvents were used in the following order, hexane > dichloromethane > ethyl acetate > acetone >ethanol > methanol > water. The micro-broth dilution method was used as an antimycobacterial susceptibility test to screen for the extract that effectively inhibited M. smegmatis growth and biofilm formation. Biofilm quantification was performed by staining the biofilms with crystal violet and determining the amount of the stain using a spectrophotometer. In addition, the effects of combining the most active extract with kanamycin were also investigated. RESULTS: The minimum inhibitory concentrations (MIC) of the extracts were found to be 6.2 μg/ml for the acetone extract, 12.5 μg/ml for both the ethanol and the total extract and 50 μg/ml for both the methanol and ethyl acetate extracts. The ethanol extract, dichloromethane extract and water extract were the only extracts that effectively inhibited biofilm formation in M. smegmatis. Combining the ethanol extract with kanamycin enhanced the effect of the ethanol extract in terms of inhibition of biofilm formation. CONCLUSIONS: P. curatellifolia leaves contain phytochemicals that have the potential to be used both as antimycobacterial and anti-biofilm formation compounds. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28558683/Inhibition_of_biofilm_formation_in_Mycobacterium_smegmatis_by_Parinari_curatellifolia_leaf_extracts_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1801-5 DB - PRIME DP - Unbound Medicine ER -