Tags

Type your tag names separated by a space and hit enter

Outbreak by Hypermucoviscous Klebsiella pneumoniae ST11 Isolates with Carbapenem Resistance in a Tertiary Hospital in China.

Abstract

Hypervirulent and multidrug resistant Klebsiella pneumoniae strains pose a significant threat to the public health. In the present study, 21 carbapenem-resistant K. pneumoniae isolates (CRKP) were determined by the string test as hypermucoviscous K. pneumoniae (HMKP), with the prevalence of 15.0% (21/140) among CRKP, and 1.1% (21/1838) among all K. pneumoniae isolates. Among them, 7 (33.3%), and 1 (4.76%) isolate belonged to capsular serotype K20 and K2 respectively, while 13 (61.9%, 13/21) weren't successfully typed by capsular serotyping. All the 21 isolates were carbapenemase-producers and were positive for blaKPC-2. In addition to blaKPC-2, all the 21 isolates except one harbor blaSHV-11, and 15 carry extended-spectrum β-lactamase gene blaCTX-M-65. The virulence-associated genes with more than 90% of positive rates among 21 isolates included ureA (100%, 21/21), wabG (100%, 21/21), fimH (95.2%, 20/21), entB (95.2%, 20/21), ycf (95.2%, 20/21), ybtS (95.2%, 20/21), and iutA (90.5%, 19/21). rmpA and aerobactin were found in 57.1% (12/21) isolates. Five sequence types (STs) were identified by multilocus sequence typing (MLST), including ST11 (11 K-non capsule typable and 5 K20 isolates), ST268 (1 K20 isolate and 1 K-non capsule typable isolate), ST65 (1 K2 isolate), ST692 (1 K-non capsule typable isolate), and ST595, a novel sequence type (1 K-non capsule typable isolate). Pulsed-field gel electrophoresis (PFGE) results showed two major PFGE clusters, of which cluster A accounts for 6 ST11 isolates (28.6%) and cluster B includes 8 ST11 isolates (38.1%, 8/21). Ten and six ST11 isolates were isolated from 2014 and 2015, respectively, while 8 were isolated from the same month of December in 2014. Ten isolates were collected from the intensive care unit (ICU), and all except one belonged to ST11. Additional 4 ST11 isolates were collected from patients in non-ICU wards, who had more than 10 days of ICU stay history in 2014 prior to transfer to their current wards where the isolates were recovered. Taken together, the present study showed a hospital outbreak and dissemination of ST11 HMKP with carbapenem resistance caused by KPC-2. Effective surveillance and strict infection control strategies should be implemented to prevent outbreak by HMKP with carbapenem resistance in hospitals.

Authors+Show Affiliations

Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.Department of Laboratory Medicine, The Second Affiliated Hospital of Nanchang UniversityNanchang, China.Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers UniversityNewark, NJ, USA.Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers UniversityNewark, NJ, USA.Department of Laboratory Medicine, The Second Affiliated Hospital of Zhejiang UniversityHangzhou, China.Department of Intensive Care Unit, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

28560183

Citation

Zhan, Lingling, et al. "Outbreak By Hypermucoviscous Klebsiella Pneumoniae ST11 Isolates With Carbapenem Resistance in a Tertiary Hospital in China." Frontiers in Cellular and Infection Microbiology, vol. 7, 2017, p. 182.
Zhan L, Wang S, Guo Y, et al. Outbreak by Hypermucoviscous Klebsiella pneumoniae ST11 Isolates with Carbapenem Resistance in a Tertiary Hospital in China. Front Cell Infect Microbiol. 2017;7:182.
Zhan, L., Wang, S., Guo, Y., Jin, Y., Duan, J., Hao, Z., Lv, J., Qi, X., Hu, L., Chen, L., Kreiswirth, B. N., Zhang, R., Pan, J., Wang, L., & Yu, F. (2017). Outbreak by Hypermucoviscous Klebsiella pneumoniae ST11 Isolates with Carbapenem Resistance in a Tertiary Hospital in China. Frontiers in Cellular and Infection Microbiology, 7, 182. https://doi.org/10.3389/fcimb.2017.00182
Zhan L, et al. Outbreak By Hypermucoviscous Klebsiella Pneumoniae ST11 Isolates With Carbapenem Resistance in a Tertiary Hospital in China. Front Cell Infect Microbiol. 2017;7:182. PubMed PMID: 28560183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Outbreak by Hypermucoviscous Klebsiella pneumoniae ST11 Isolates with Carbapenem Resistance in a Tertiary Hospital in China. AU - Zhan,Lingling, AU - Wang,Shanshan, AU - Guo,Yinjuan, AU - Jin,Ye, AU - Duan,Jingjing, AU - Hao,Zhihao, AU - Lv,Jingnan, AU - Qi,Xiuqin, AU - Hu,Longhua, AU - Chen,Liang, AU - Kreiswirth,Barry N, AU - Zhang,Rong, AU - Pan,Jingye, AU - Wang,Liangxing, AU - Yu,Fangyou, Y1 - 2017/05/16/ PY - 2017/01/22/received PY - 2017/04/27/accepted PY - 2017/6/1/entrez PY - 2017/6/1/pubmed PY - 2018/1/16/medline KW - KPC-2 KW - Klesiella pneumoniae KW - carbapenem resistance KW - epidemiology KW - hypermucoviscous SP - 182 EP - 182 JF - Frontiers in cellular and infection microbiology JO - Front Cell Infect Microbiol VL - 7 N2 - Hypervirulent and multidrug resistant Klebsiella pneumoniae strains pose a significant threat to the public health. In the present study, 21 carbapenem-resistant K. pneumoniae isolates (CRKP) were determined by the string test as hypermucoviscous K. pneumoniae (HMKP), with the prevalence of 15.0% (21/140) among CRKP, and 1.1% (21/1838) among all K. pneumoniae isolates. Among them, 7 (33.3%), and 1 (4.76%) isolate belonged to capsular serotype K20 and K2 respectively, while 13 (61.9%, 13/21) weren't successfully typed by capsular serotyping. All the 21 isolates were carbapenemase-producers and were positive for blaKPC-2. In addition to blaKPC-2, all the 21 isolates except one harbor blaSHV-11, and 15 carry extended-spectrum β-lactamase gene blaCTX-M-65. The virulence-associated genes with more than 90% of positive rates among 21 isolates included ureA (100%, 21/21), wabG (100%, 21/21), fimH (95.2%, 20/21), entB (95.2%, 20/21), ycf (95.2%, 20/21), ybtS (95.2%, 20/21), and iutA (90.5%, 19/21). rmpA and aerobactin were found in 57.1% (12/21) isolates. Five sequence types (STs) were identified by multilocus sequence typing (MLST), including ST11 (11 K-non capsule typable and 5 K20 isolates), ST268 (1 K20 isolate and 1 K-non capsule typable isolate), ST65 (1 K2 isolate), ST692 (1 K-non capsule typable isolate), and ST595, a novel sequence type (1 K-non capsule typable isolate). Pulsed-field gel electrophoresis (PFGE) results showed two major PFGE clusters, of which cluster A accounts for 6 ST11 isolates (28.6%) and cluster B includes 8 ST11 isolates (38.1%, 8/21). Ten and six ST11 isolates were isolated from 2014 and 2015, respectively, while 8 were isolated from the same month of December in 2014. Ten isolates were collected from the intensive care unit (ICU), and all except one belonged to ST11. Additional 4 ST11 isolates were collected from patients in non-ICU wards, who had more than 10 days of ICU stay history in 2014 prior to transfer to their current wards where the isolates were recovered. Taken together, the present study showed a hospital outbreak and dissemination of ST11 HMKP with carbapenem resistance caused by KPC-2. Effective surveillance and strict infection control strategies should be implemented to prevent outbreak by HMKP with carbapenem resistance in hospitals. SN - 2235-2988 UR - https://www.unboundmedicine.com/medline/citation/28560183/Outbreak_by_Hypermucoviscous_Klebsiella_pneumoniae_ST11_Isolates_with_Carbapenem_Resistance_in_a_Tertiary_Hospital_in_China_ L2 - https://doi.org/10.3389/fcimb.2017.00182 DB - PRIME DP - Unbound Medicine ER -