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Erythrocyte pyrimidine 5'-nucleotidase inhibition by acute lead exposure in neonatal rats.

Abstract

Inhibition by lead of erythrocyte pyrimidine 5'-nucleotidase (P5N) is thought to contribute to morphological abnormalities observed in red blood cells (RBC) of lead-exposed subjects. However, neither the mechanism of lead inhibition of P5N nor the relationship of this inhibition to blood lead levels attained in exposed subjects is known. In the present investigation, acute in vivo and in vitro lead acetate effects on erythrocyte P5N from 21-day-old rat pups were determined and were related to blood lead concentrations ascertained by atomic absorption spectrophotometry. Acute lead administration to rat pups resulted in a 16% to 21% reduction in erythrocyte P5N, with mean blood lead levels ranging from 77 to 108 micrograms/dl 24 hours later. Inhibition of erythrocyte P5N was linearly related to blood lead level (r = -0.67, P less than 0.05) following acute lead administration. Lead acetate addition to RBC preparations from 21-day-old rats resulted in concentration-dependent P5N inhibition which was comparable to that produced following acute in vivo exposure. The results indicate that acute P5N inhibition in lead-treated neonatal rats is due to noncompetitive P5N inhibition by lead. The inhibition of P5N produced by acute lead treatment is linearly related to blood lead concentrations.

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  • Authors+Show Affiliations

    ,

    Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions, Wayne State University, Detroit, MI 48202.

    ,

    Source

    MeSH

    5'-Nucleotidase
    Animals
    Animals, Newborn
    Cytidine Monophosphate
    Erythrocytes
    Lead
    Organometallic Compounds
    Rats

    Pub Type(s)

    Journal Article
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    2856073

    Citation

    Konantakieti, C, et al. "Erythrocyte Pyrimidine 5'-nucleotidase Inhibition By Acute Lead Exposure in Neonatal Rats." Journal of Biochemical Toxicology, vol. 1, no. 3, 1986, pp. 51-9.
    Konantakieti C, Beuthin FC, Louis-Ferdinand RT. Erythrocyte pyrimidine 5'-nucleotidase inhibition by acute lead exposure in neonatal rats. J Biochem Toxicol. 1986;1(3):51-9.
    Konantakieti, C., Beuthin, F. C., & Louis-Ferdinand, R. T. (1986). Erythrocyte pyrimidine 5'-nucleotidase inhibition by acute lead exposure in neonatal rats. Journal of Biochemical Toxicology, 1(3), pp. 51-9.
    Konantakieti C, Beuthin FC, Louis-Ferdinand RT. Erythrocyte Pyrimidine 5'-nucleotidase Inhibition By Acute Lead Exposure in Neonatal Rats. J Biochem Toxicol. 1986;1(3):51-9. PubMed PMID: 2856073.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Erythrocyte pyrimidine 5'-nucleotidase inhibition by acute lead exposure in neonatal rats. AU - Konantakieti,C, AU - Beuthin,F C, AU - Louis-Ferdinand,R T, PY - 1986/9/1/pubmed PY - 1986/9/1/medline PY - 1986/9/1/entrez SP - 51 EP - 9 JF - Journal of biochemical toxicology JO - J. Biochem. Toxicol. VL - 1 IS - 3 N2 - Inhibition by lead of erythrocyte pyrimidine 5'-nucleotidase (P5N) is thought to contribute to morphological abnormalities observed in red blood cells (RBC) of lead-exposed subjects. However, neither the mechanism of lead inhibition of P5N nor the relationship of this inhibition to blood lead levels attained in exposed subjects is known. In the present investigation, acute in vivo and in vitro lead acetate effects on erythrocyte P5N from 21-day-old rat pups were determined and were related to blood lead concentrations ascertained by atomic absorption spectrophotometry. Acute lead administration to rat pups resulted in a 16% to 21% reduction in erythrocyte P5N, with mean blood lead levels ranging from 77 to 108 micrograms/dl 24 hours later. Inhibition of erythrocyte P5N was linearly related to blood lead level (r = -0.67, P less than 0.05) following acute lead administration. Lead acetate addition to RBC preparations from 21-day-old rats resulted in concentration-dependent P5N inhibition which was comparable to that produced following acute in vivo exposure. The results indicate that acute P5N inhibition in lead-treated neonatal rats is due to noncompetitive P5N inhibition by lead. The inhibition of P5N produced by acute lead treatment is linearly related to blood lead concentrations. SN - 0887-2082 UR - https://www.unboundmedicine.com/medline/citation/2856073/Erythrocyte_pyrimidine_5'_nucleotidase_inhibition_by_acute_lead_exposure_in_neonatal_rats_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0887-2082&date=1986&volume=1&issue=3&spage=51 DB - PRIME DP - Unbound Medicine ER -