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The four-transmembrane protein MAL2 and tumor protein D52 (TPD52) are highly expressed in colorectal cancer and correlated with poor prognosis.
PLoS One. 2017; 12(5):e0178515.Plos

Abstract

The four-transmembrane protein MAL2 and tumor protein D52 (TPD52) have been shown to be involved in tumorigenesis of various cancers. However, their roles in colorectal cancer (CRC) remain unclear. In this study, we explored the expressions of MAL2 and TPD52 in tumor specimens resected from 123 CRC patients and the prognostic values of the two proteins in CRC. Immunohistochemical analyses showed that MAL2 (P<0.001) and TPD52 (P<0.001) were significantly highly expressed in primary carcinoma tissues compared with adjacent non-cancerous mucosa tissues. And TPD52 exhibited frequent overexpression in liver metastasis tissues relative to primary carcinoma tissues (P = 0.042), while MAL2 in lymphnode and liver metastasis tissues showed no significant elevation. Real-time quantitative PCR (RT-qPCR) showed the identical results. Correlation analyses by Pearson's chi-square test demonstrated that MAL2 in tumors was positively correlated with tumor status (pathological assessment of regional lymph nodes (pN, P = 0.024)), and clinic stage (P = 0.017). Additionally, the expression of TPD52 was detected under the same condition and was shown to be positively correlated withtumor status (pathological assessment of the primary tumor (pT, P = 0.035), distant metastasis (pM, P = 0.001)) and CRC clinicopathology(P = 0.024). Kaplan-Meier survival curves indicated that positive MAL2 (P<0.001) and TPD52 (P<0.001) expressions were associated with poor overall survival (OS) in CRC patients. Multivariate analysis showed that MAL2 and TPD52 expression was an independent prognostic factor for reduced OS of CRC patients. Moreover, overexpression of TPD52 in CRC SW480 cells showed an increased cell migration (P = 0.023) and invasion (P = 0.012) through inducing occurrence of epithelial-mesenchymal transition (EMT) and activating focal adhesion kinase (FAK)-mediated integrin signalling and PI3K⁄Akt signalling.Whereas TPD52-depleted cells showed the reverse effect. These data suggested that MAL2 and TPD52 might be potential biomarkers for clinical prognosis and might be a promising therapeutic target for CRC.

Authors+Show Affiliations

Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28562687

Citation

Li, Jingwen, et al. "The Four-transmembrane Protein MAL2 and Tumor Protein D52 (TPD52) Are Highly Expressed in Colorectal Cancer and Correlated With Poor Prognosis." PloS One, vol. 12, no. 5, 2017, pp. e0178515.
Li J, Li Y, Liu H, et al. The four-transmembrane protein MAL2 and tumor protein D52 (TPD52) are highly expressed in colorectal cancer and correlated with poor prognosis. PLoS One. 2017;12(5):e0178515.
Li, J., Li, Y., Liu, H., Liu, Y., & Cui, B. (2017). The four-transmembrane protein MAL2 and tumor protein D52 (TPD52) are highly expressed in colorectal cancer and correlated with poor prognosis. PloS One, 12(5), e0178515. https://doi.org/10.1371/journal.pone.0178515
Li J, et al. The Four-transmembrane Protein MAL2 and Tumor Protein D52 (TPD52) Are Highly Expressed in Colorectal Cancer and Correlated With Poor Prognosis. PLoS One. 2017;12(5):e0178515. PubMed PMID: 28562687.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The four-transmembrane protein MAL2 and tumor protein D52 (TPD52) are highly expressed in colorectal cancer and correlated with poor prognosis. AU - Li,Jingwen, AU - Li,Yongmin, AU - Liu,He, AU - Liu,Yanlong, AU - Cui,Binbin, Y1 - 2017/05/31/ PY - 2017/04/01/received PY - 2017/05/15/accepted PY - 2017/6/1/entrez PY - 2017/6/1/pubmed PY - 2017/9/28/medline SP - e0178515 EP - e0178515 JF - PloS one JO - PLoS One VL - 12 IS - 5 N2 - The four-transmembrane protein MAL2 and tumor protein D52 (TPD52) have been shown to be involved in tumorigenesis of various cancers. However, their roles in colorectal cancer (CRC) remain unclear. In this study, we explored the expressions of MAL2 and TPD52 in tumor specimens resected from 123 CRC patients and the prognostic values of the two proteins in CRC. Immunohistochemical analyses showed that MAL2 (P<0.001) and TPD52 (P<0.001) were significantly highly expressed in primary carcinoma tissues compared with adjacent non-cancerous mucosa tissues. And TPD52 exhibited frequent overexpression in liver metastasis tissues relative to primary carcinoma tissues (P = 0.042), while MAL2 in lymphnode and liver metastasis tissues showed no significant elevation. Real-time quantitative PCR (RT-qPCR) showed the identical results. Correlation analyses by Pearson's chi-square test demonstrated that MAL2 in tumors was positively correlated with tumor status (pathological assessment of regional lymph nodes (pN, P = 0.024)), and clinic stage (P = 0.017). Additionally, the expression of TPD52 was detected under the same condition and was shown to be positively correlated withtumor status (pathological assessment of the primary tumor (pT, P = 0.035), distant metastasis (pM, P = 0.001)) and CRC clinicopathology(P = 0.024). Kaplan-Meier survival curves indicated that positive MAL2 (P<0.001) and TPD52 (P<0.001) expressions were associated with poor overall survival (OS) in CRC patients. Multivariate analysis showed that MAL2 and TPD52 expression was an independent prognostic factor for reduced OS of CRC patients. Moreover, overexpression of TPD52 in CRC SW480 cells showed an increased cell migration (P = 0.023) and invasion (P = 0.012) through inducing occurrence of epithelial-mesenchymal transition (EMT) and activating focal adhesion kinase (FAK)-mediated integrin signalling and PI3K⁄Akt signalling.Whereas TPD52-depleted cells showed the reverse effect. These data suggested that MAL2 and TPD52 might be potential biomarkers for clinical prognosis and might be a promising therapeutic target for CRC. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28562687/The_four_transmembrane_protein_MAL2_and_tumor_protein_D52__TPD52__are_highly_expressed_in_colorectal_cancer_and_correlated_with_poor_prognosis_ L2 - https://dx.plos.org/10.1371/journal.pone.0178515 DB - PRIME DP - Unbound Medicine ER -