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ANALYSIS OF PHENOLIC AND FLAVONOIDS OF WILD EPHEDRA ALATA PLANT EXTRACTS BY LC/PDA AND LC/MS AND THEIR ANTIOXIDANT ACTIVITY.
Afr J Tradit Complement Altern Med. 2017; 14(2):130-141.AJ

Abstract

BACKGROUND

Ephedra is among Palestinian medicinal plants that are traditionally used in folkloric medicine for treating many diseases. Ephedra is known to have antibacterial and antioxidant effects. The goal of this study is to evaluate the antioxidant activity of different extracts from the Ephedra alata plant growing wild in Palestine, and to analyze their phenolic and flavonoid constituents by HPLC/PDA and HPLC/MS.

MATERIALS AND METHODS

Samples of the Ephedra alata plant grown wild in Palestine were extracted with three different solvents namely, 100% water, 80% ethanol, and 100% ethanol. The extracts were analyzed for their total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AA), as well as phenolic and flavonoids content by HPLC/PDA/MS.

RESULTS

The results revealed that the polarity of the extraction solvent affects the TPC, TFC, and AA of extracts. It was found that both TPC and AA are highest for plant extracted with 80% ethanol, followed by 100% ethanol, and finally with 100% water. TFC however was highest in the following order: 100% ethanol > 80% ethanol > water. Pearson correlation indicated that there is a significant correlation between AA and TPC, but there is no correlation between AA and TFC. Simultaneous HPLC-PDA and UHPLC-MS analysis of the ethanolic plant extracts revealed the presence of Luteolin-7-O-glucuronide flavone, Myricetin 3-rhamnoside and some other major polyphenolic compounds that share myricetin skeleton.

CONCLUSION

Ephedra alata extract is rich in potent falvonoid glycosidic compounds as revealed by their similar overlaid UV-Vis spectra and UHPLC-MS results. On the basis of these findings, it is concluded that Ephedra alata constitutes a natural source of potent antioxidants that may prevent many diseases and could be potentially used in food, cosmetics, and pharmaceutical products.

Authors+Show Affiliations

Chemistry Department, Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, Jerusalem, Palestine.Faculty of Pharmacy, Al-Quds University, P.O. Box 20002, Jerusalem, Palestine.Biology Department, Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, Jerusalem, Palestine.Chemistry Department, Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, Jerusalem, Palestine.Al Quds-Bard College, Al-Quds University, Abu Dies, Jerusalem, Palestine.Chemistry Department, Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, Jerusalem, Palestine.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28573229

Citation

Al-Rimawi, Fuad, et al. "ANALYSIS of PHENOLIC and FLAVONOIDS of WILD EPHEDRA ALATA PLANT EXTRACTS BY LC/PDA and LC/MS and THEIR ANTIOXIDANT ACTIVITY." African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, vol. 14, no. 2, 2017, pp. 130-141.
Al-Rimawi F, Abu-Lafi S, Abbadi J, et al. ANALYSIS OF PHENOLIC AND FLAVONOIDS OF WILD EPHEDRA ALATA PLANT EXTRACTS BY LC/PDA AND LC/MS AND THEIR ANTIOXIDANT ACTIVITY. Afr J Tradit Complement Altern Med. 2017;14(2):130-141.
Al-Rimawi, F., Abu-Lafi, S., Abbadi, J., Alamarneh, A. A. A., Sawahreh, R. A., & Odeh, I. (2017). ANALYSIS OF PHENOLIC AND FLAVONOIDS OF WILD EPHEDRA ALATA PLANT EXTRACTS BY LC/PDA AND LC/MS AND THEIR ANTIOXIDANT ACTIVITY. African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, 14(2), 130-141. https://doi.org/10.21010/ajtcam.v14i2.14
Al-Rimawi F, et al. ANALYSIS of PHENOLIC and FLAVONOIDS of WILD EPHEDRA ALATA PLANT EXTRACTS BY LC/PDA and LC/MS and THEIR ANTIOXIDANT ACTIVITY. Afr J Tradit Complement Altern Med. 2017;14(2):130-141. PubMed PMID: 28573229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ANALYSIS OF PHENOLIC AND FLAVONOIDS OF WILD EPHEDRA ALATA PLANT EXTRACTS BY LC/PDA AND LC/MS AND THEIR ANTIOXIDANT ACTIVITY. AU - Al-Rimawi,Fuad, AU - Abu-Lafi,Saleh, AU - Abbadi,Jehad, AU - Alamarneh,Ayman A A, AU - Sawahreh,Raneen A, AU - Odeh,Imad, Y1 - 2017/01/13/ PY - 2017/6/3/entrez PY - 2017/6/3/pubmed PY - 2017/8/17/medline KW - Ephedra Alata KW - HPLC KW - LC/MS KW - antioxidant activity KW - flavonoid content KW - phenolic content SP - 130 EP - 141 JF - African journal of traditional, complementary, and alternative medicines : AJTCAM JO - Afr J Tradit Complement Altern Med VL - 14 IS - 2 N2 - BACKGROUND: Ephedra is among Palestinian medicinal plants that are traditionally used in folkloric medicine for treating many diseases. Ephedra is known to have antibacterial and antioxidant effects. The goal of this study is to evaluate the antioxidant activity of different extracts from the Ephedra alata plant growing wild in Palestine, and to analyze their phenolic and flavonoid constituents by HPLC/PDA and HPLC/MS. MATERIALS AND METHODS: Samples of the Ephedra alata plant grown wild in Palestine were extracted with three different solvents namely, 100% water, 80% ethanol, and 100% ethanol. The extracts were analyzed for their total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AA), as well as phenolic and flavonoids content by HPLC/PDA/MS. RESULTS: The results revealed that the polarity of the extraction solvent affects the TPC, TFC, and AA of extracts. It was found that both TPC and AA are highest for plant extracted with 80% ethanol, followed by 100% ethanol, and finally with 100% water. TFC however was highest in the following order: 100% ethanol > 80% ethanol > water. Pearson correlation indicated that there is a significant correlation between AA and TPC, but there is no correlation between AA and TFC. Simultaneous HPLC-PDA and UHPLC-MS analysis of the ethanolic plant extracts revealed the presence of Luteolin-7-O-glucuronide flavone, Myricetin 3-rhamnoside and some other major polyphenolic compounds that share myricetin skeleton. CONCLUSION: Ephedra alata extract is rich in potent falvonoid glycosidic compounds as revealed by their similar overlaid UV-Vis spectra and UHPLC-MS results. On the basis of these findings, it is concluded that Ephedra alata constitutes a natural source of potent antioxidants that may prevent many diseases and could be potentially used in food, cosmetics, and pharmaceutical products. SN - 2505-0044 UR - https://www.unboundmedicine.com/medline/citation/28573229/ANALYSIS_OF_PHENOLIC_AND_FLAVONOIDS_OF_WILD_EPHEDRA_ALATA_PLANT_EXTRACTS_BY_LC/PDA_AND_LC/MS_AND_THEIR_ANTIOXIDANT_ACTIVITY_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28573229/ DB - PRIME DP - Unbound Medicine ER -