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Angelica tenuissima Nakai Ameliorates Cognitive Impairment and Promotes Neurogenesis in Mouse Model of Alzheimer's Disease.
Chin J Integr Med 2018; 24(5):378-384CJ

Abstract

OBJECTIVE

To research Angelica tenuissima Nakai (ATN) for use in novel Alzheimer's disease (AD) therapeutics.

METHODS

The effect of a 30% ethanol extract of ATN (KH032) on AD-like cognitive impairment and neuropathological and neuroinflammatory changes induced by bilateral intracerebroventricular injections of β-amyloid (Aβ) peptide (Aβ1-42) was investigated. Male C57Bl/6 mice were randomly divided into 4 groups, 10 in each group. KH032-treated groups were administrated with a low or high dose of KH032 (50 and 200 mg/kg, respectively), intragastrically for 16 days; distilled water was applied in the sham and negative groups. Open fifield test, Y maze and Morris water maze test were used for behavior test and cognitive ability. In addition, the neuroprotective effects of KH032 in Aβ1-42-infused mice on the histopathological markers [neuronspecific nuclear protein (NeuN), Aβ1-42] of neurodegeneration were examined. The levels of glial fibrillary acidic protein (GFAP), NeuN, phosphorylation extracellular signal-regulated kinase (ERK)/ERK, brain-derived neurotrophic factor (BDNF), phosphorylation cAMP response element-binding (CREB)/CREB protein expression were measured by Western blot.

RESULTS

KH032 treatment ameliorated cognitive impairments, reduced the overexpression of Aβ1-42, and inhibited neuronal loss and neuroinflammatory response in the Aβ1-42-infused mice. Moreover, KH032 treatment enhanced BDNF expression levels in the hippocampus. Finally, KH032 treatment increased phosphorylation of ERK1/2 and CREB, vital for ERK-CREB signaling.

CONCLUSIONS

KH032 attenuated cognitive defificits in the Aβ1-42-infused mice by increasing BDNF expression and ERK1/2 and CREB phosphorylation and inhibiting neuronal loss and neuroinflflammatory response, suggesting that KH032 has therapeutic potential in neurodegenerative disorders such as AD.

Authors+Show Affiliations

Department of Psychiatry, Hospital of Korean Medicine, Kyung Hee University Medical Center, Seoul, 130-872, South Korea.Department of Psychiatry, Hospital of Korean Medicine, Kyung Hee University Medical Center, Seoul, 130-872, South Korea.Department of Psychiatry, Hospital of Korean Medicine, Kyung Hee University Medical Center, Seoul, 130-872, South Korea. chosh@khmc.or.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28578486

Citation

Choi, Minji, et al. "Angelica Tenuissima Nakai Ameliorates Cognitive Impairment and Promotes Neurogenesis in Mouse Model of Alzheimer's Disease." Chinese Journal of Integrative Medicine, vol. 24, no. 5, 2018, pp. 378-384.
Choi M, Lee Y, Cho SH. Angelica tenuissima Nakai Ameliorates Cognitive Impairment and Promotes Neurogenesis in Mouse Model of Alzheimer's Disease. Chin J Integr Med. 2018;24(5):378-384.
Choi, M., Lee, Y., & Cho, S. H. (2018). Angelica tenuissima Nakai Ameliorates Cognitive Impairment and Promotes Neurogenesis in Mouse Model of Alzheimer's Disease. Chinese Journal of Integrative Medicine, 24(5), pp. 378-384. doi:10.1007/s11655-017-2812-2.
Choi M, Lee Y, Cho SH. Angelica Tenuissima Nakai Ameliorates Cognitive Impairment and Promotes Neurogenesis in Mouse Model of Alzheimer's Disease. Chin J Integr Med. 2018;24(5):378-384. PubMed PMID: 28578486.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Angelica tenuissima Nakai Ameliorates Cognitive Impairment and Promotes Neurogenesis in Mouse Model of Alzheimer's Disease. AU - Choi,Minji, AU - Lee,Younghyurk, AU - Cho,Seung-Hun, Y1 - 2017/06/03/ PY - 2014/06/14/received PY - 2017/6/5/pubmed PY - 2018/10/6/medline PY - 2017/6/5/entrez KW - Alzheimer’s disease KW - Angelica tenuissima Nakai KW - beta amyloid KW - cognitive impairment KW - neurogenesis SP - 378 EP - 384 JF - Chinese journal of integrative medicine JO - Chin J Integr Med VL - 24 IS - 5 N2 - OBJECTIVE: To research Angelica tenuissima Nakai (ATN) for use in novel Alzheimer's disease (AD) therapeutics. METHODS: The effect of a 30% ethanol extract of ATN (KH032) on AD-like cognitive impairment and neuropathological and neuroinflammatory changes induced by bilateral intracerebroventricular injections of β-amyloid (Aβ) peptide (Aβ1-42) was investigated. Male C57Bl/6 mice were randomly divided into 4 groups, 10 in each group. KH032-treated groups were administrated with a low or high dose of KH032 (50 and 200 mg/kg, respectively), intragastrically for 16 days; distilled water was applied in the sham and negative groups. Open fifield test, Y maze and Morris water maze test were used for behavior test and cognitive ability. In addition, the neuroprotective effects of KH032 in Aβ1-42-infused mice on the histopathological markers [neuronspecific nuclear protein (NeuN), Aβ1-42] of neurodegeneration were examined. The levels of glial fibrillary acidic protein (GFAP), NeuN, phosphorylation extracellular signal-regulated kinase (ERK)/ERK, brain-derived neurotrophic factor (BDNF), phosphorylation cAMP response element-binding (CREB)/CREB protein expression were measured by Western blot. RESULTS: KH032 treatment ameliorated cognitive impairments, reduced the overexpression of Aβ1-42, and inhibited neuronal loss and neuroinflammatory response in the Aβ1-42-infused mice. Moreover, KH032 treatment enhanced BDNF expression levels in the hippocampus. Finally, KH032 treatment increased phosphorylation of ERK1/2 and CREB, vital for ERK-CREB signaling. CONCLUSIONS: KH032 attenuated cognitive defificits in the Aβ1-42-infused mice by increasing BDNF expression and ERK1/2 and CREB phosphorylation and inhibiting neuronal loss and neuroinflflammatory response, suggesting that KH032 has therapeutic potential in neurodegenerative disorders such as AD. SN - 1672-0415 UR - https://www.unboundmedicine.com/medline/citation/28578486/Angelica_tenuissima_Nakai_Ameliorates_Cognitive_Impairment_and_Promotes_Neurogenesis_in_Mouse_Model_of_Alzheimer's_Disease_ L2 - https://dx.doi.org/10.1007/s11655-017-2812-2 DB - PRIME DP - Unbound Medicine ER -