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Terpene constituents of the aerial parts, phenolic content, antibacterial potential, free radical scavenging and antioxidant activity of Callistemon citrinus (Curtis) Skeels (Myrtaceae) from Eastern Cape Province of South Africa.
BMC Complement Altern Med 2017; 17(1):292BC

Abstract

BACKGROUND

Volatile oil from aromatic plants has been used by ancient Egyptians in embalming for the inhibition of bacterial growth and prevention of decay, Callistemon citrinus is used in traditional therapies for the treatment of bronchitis, cough, inflammation and as an antimicrobial herbs. This study examines the essential constituents of the volatile oils obtained from the aerial parts of the plant as well as its antioxidant activity, free radical scavenging, phenolic content and the antibacterial potential of the oils.

METHODS

A portion of 500 g, 250 g and 150 g of the leaves, flowers and stems of this plant respectively were subjected to hydro-distillation process for three hours. The oils collected from the various plant parts were immediately subjected to GC-MS analysis. The overall phenolic content of the leaves oil, radical scavenging, antibacterial action and antioxidant activities of the essential oils of both the leaves and flowers of Callistemon citrinus were determined using standard methods, with free radical DPPH and ABTS as a reference antioxidant.

RESULTS

Analyses of the three oils revealed a total of twenty-six components for the leaves oil representing 96.84% of the total oil composition, forty-one components for the flowers oil accounting for 98.92% of the whole composition and ten components for the stem oil amounting to 99.98% of the entire oil constituents. The dominant compounds in the leaves oil were eucalyptol (48.98%) and α-terpineol (8.01%), while α-eudesmol (12.93%), caryophyllene (11.89%), (-)-bornyl-acetate (10.02%) and eucalyptol (8.11%) were the main constituents of the flowers oil. In the same vein, the leading constituents in the stems oil were eucalyptol (56.00%) and α-pinene (31.03%). The antioxidant capacities of both the leaves and flowers oils of the plant were evaluated and their IC50 were (1.49 and 1.13) for DPPH and (0.14 and 0.03) for ABTS assay respectively. The antibacterial activities of the oils from the (leaves and flowers) were also examined and were found to have wide range of activities against the bacterial strains used in this study.

CONCLUSION

Observations drawn from this experiment shows clearly that the leaves and flowers of Callistemon citrinus possess phenolic compounds and cyclic ether of several pharmacological behaviors.

Authors+Show Affiliations

Department of Pure and Applied Chemistry, University of Fort Hare, Alice, 5700, South Africa. timlarayetan@gmail.com. Chemistry Department, Kogi State University, Anyigba, Kogi, Nigeria. timlarayetan@gmail.com.Department of Pure and Applied Chemistry, University of Fort Hare, Alice, 5700, South Africa.Department of Pure and Applied Chemistry, University of Fort Hare, Alice, 5700, South Africa.SAMRC Microbial Water Quality Monitoring Center, University of Fort Hare, Alice, Eastern Cape, South Africa. Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice, Eastern Cape, South Africa.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28583128

Citation

Larayetan, Rotimi A., et al. "Terpene Constituents of the Aerial Parts, Phenolic Content, Antibacterial Potential, Free Radical Scavenging and Antioxidant Activity of Callistemon Citrinus (Curtis) Skeels (Myrtaceae) From Eastern Cape Province of South Africa." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 292.
Larayetan RA, Okoh OO, Sadimenko A, et al. Terpene constituents of the aerial parts, phenolic content, antibacterial potential, free radical scavenging and antioxidant activity of Callistemon citrinus (Curtis) Skeels (Myrtaceae) from Eastern Cape Province of South Africa. BMC Complement Altern Med. 2017;17(1):292.
Larayetan, R. A., Okoh, O. O., Sadimenko, A., & Okoh, A. I. (2017). Terpene constituents of the aerial parts, phenolic content, antibacterial potential, free radical scavenging and antioxidant activity of Callistemon citrinus (Curtis) Skeels (Myrtaceae) from Eastern Cape Province of South Africa. BMC Complementary and Alternative Medicine, 17(1), p. 292. doi:10.1186/s12906-017-1804-2.
Larayetan RA, et al. Terpene Constituents of the Aerial Parts, Phenolic Content, Antibacterial Potential, Free Radical Scavenging and Antioxidant Activity of Callistemon Citrinus (Curtis) Skeels (Myrtaceae) From Eastern Cape Province of South Africa. BMC Complement Altern Med. 2017 Jun 5;17(1):292. PubMed PMID: 28583128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Terpene constituents of the aerial parts, phenolic content, antibacterial potential, free radical scavenging and antioxidant activity of Callistemon citrinus (Curtis) Skeels (Myrtaceae) from Eastern Cape Province of South Africa. AU - Larayetan,Rotimi A, AU - Okoh,Omobola O, AU - Sadimenko,Alexander, AU - Okoh,Anthony I, Y1 - 2017/06/05/ PY - 2017/02/13/received PY - 2017/05/23/accepted PY - 2017/6/7/entrez PY - 2017/6/7/pubmed PY - 2017/8/22/medline KW - ABTS KW - Antimicrobial KW - Antioxidant KW - DPPH KW - Hydro distillation KW - Opc KW - Volatile oil SP - 292 EP - 292 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Volatile oil from aromatic plants has been used by ancient Egyptians in embalming for the inhibition of bacterial growth and prevention of decay, Callistemon citrinus is used in traditional therapies for the treatment of bronchitis, cough, inflammation and as an antimicrobial herbs. This study examines the essential constituents of the volatile oils obtained from the aerial parts of the plant as well as its antioxidant activity, free radical scavenging, phenolic content and the antibacterial potential of the oils. METHODS: A portion of 500 g, 250 g and 150 g of the leaves, flowers and stems of this plant respectively were subjected to hydro-distillation process for three hours. The oils collected from the various plant parts were immediately subjected to GC-MS analysis. The overall phenolic content of the leaves oil, radical scavenging, antibacterial action and antioxidant activities of the essential oils of both the leaves and flowers of Callistemon citrinus were determined using standard methods, with free radical DPPH and ABTS as a reference antioxidant. RESULTS: Analyses of the three oils revealed a total of twenty-six components for the leaves oil representing 96.84% of the total oil composition, forty-one components for the flowers oil accounting for 98.92% of the whole composition and ten components for the stem oil amounting to 99.98% of the entire oil constituents. The dominant compounds in the leaves oil were eucalyptol (48.98%) and α-terpineol (8.01%), while α-eudesmol (12.93%), caryophyllene (11.89%), (-)-bornyl-acetate (10.02%) and eucalyptol (8.11%) were the main constituents of the flowers oil. In the same vein, the leading constituents in the stems oil were eucalyptol (56.00%) and α-pinene (31.03%). The antioxidant capacities of both the leaves and flowers oils of the plant were evaluated and their IC50 were (1.49 and 1.13) for DPPH and (0.14 and 0.03) for ABTS assay respectively. The antibacterial activities of the oils from the (leaves and flowers) were also examined and were found to have wide range of activities against the bacterial strains used in this study. CONCLUSION: Observations drawn from this experiment shows clearly that the leaves and flowers of Callistemon citrinus possess phenolic compounds and cyclic ether of several pharmacological behaviors. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28583128/Terpene_constituents_of_the_aerial_parts_phenolic_content_antibacterial_potential_free_radical_scavenging_and_antioxidant_activity_of_Callistemon_citrinus__Curtis__Skeels__Myrtaceae__from_Eastern_Cape_Province_of_South_Africa_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1804-2 DB - PRIME DP - Unbound Medicine ER -