Tags

Type your tag names separated by a space and hit enter

The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027.
J Microbiol Immunol Infect. 2018 Jun; 51(3):344-351.JM

Abstract

PURPOSE

To characterise and compare twenty-eight Finnish Clostridium difficile RT027-like isolates, selected based on the presence of 18 bp deletion in the tcdC gene and toxin gene profile (A, B, binary), with eleven RT027 isolates from different Finnish geographical areas and time periods.

METHODS

Twenty-eight C. difficile RT027-like isolates and 11 RT027 comparative strains were characterised by capillary-electrophoresis (CE) ribotyping, multi-locus variable tandem-repeats analysis (MLVA), multi-locus sequence typing (MLST), and sequencing of tcdC and gyrA gene fragments. Susceptibility to moxifloxacin was determined by E-test.

RESULTS

Of 28 RT027-like isolates, seven RTs (016, 034, 075, 080, 153, 176 and 328), three WEBRIBO types (411, 475, AI-78) and three new profiles (F1-F3) were identified. MLVA revealed six clonal complexes (RTs 016, 027, 176 and F3). MLST showed eleven sequence types (1, 41, 47, 67, 95, 191,192, 223, 229, 264 and new ST). Twenty-two isolates (RTs 016, 080, 176, 328, F1, F2, F3 and WRTAI-78) carried Δ117 in the tcdC gene. Isolates of RTs 016, 027 and 176 were moxifloxacin resistant and harboured Thr82Ile in the GyrA.

CONCLUSION

Our results show a high diversity within 28 Finnish RT027-like C. difficile isolates, with twelve CE-ribotyping profiles and eleven STs. MLVA revealed the regional spread of RTs 016, 027, 176 and F3. The presence of Δ117 in the tcdC gene in eight non-027 RTs highlights the importance of careful interpretation of the results from molecular systems targeting this site in the genome of C. difficile and the need of strain typing for epidemiological purposes.

Authors+Show Affiliations

Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Czech Republic; DNA Laboratory, Department of Paediatric Neurology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Czech Republic. Electronic address: marcela.krutova@lfmotol.cuni.cz.Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Czech Republic.Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Czech Republic.Leiden University Medical Centre, Leiden, The Netherlands.Bacterial Infections Unit, Department of Infectious Diseases, National Institute for Health and Welfare, Helsinki, Finland.Bacterial Infections Unit, Department of Infectious Diseases, National Institute for Health and Welfare, Helsinki, Finland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28583353

Citation

Krutova, Marcela, et al. "The Recognition and Characterisation of Finnish Clostridium Difficile Isolates Resembling PCR-ribotype 027." Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi, vol. 51, no. 3, 2018, pp. 344-351.
Krutova M, Nyc O, Matejkova J, et al. The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027. J Microbiol Immunol Infect. 2018;51(3):344-351.
Krutova, M., Nyc, O., Matejkova, J., Kuijper, E. J., Jalava, J., & Mentula, S. (2018). The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027. Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi, 51(3), 344-351. https://doi.org/10.1016/j.jmii.2017.02.002
Krutova M, et al. The Recognition and Characterisation of Finnish Clostridium Difficile Isolates Resembling PCR-ribotype 027. J Microbiol Immunol Infect. 2018;51(3):344-351. PubMed PMID: 28583353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027. AU - Krutova,Marcela, AU - Nyc,Otakar, AU - Matejkova,Jana, AU - Kuijper,Ed J, AU - Jalava,Jari, AU - Mentula,Silja, Y1 - 2017/05/24/ PY - 2016/10/12/received PY - 2017/01/03/revised PY - 2017/02/06/accepted PY - 2017/6/7/pubmed PY - 2018/10/10/medline PY - 2017/6/7/entrez KW - Capillary-electrophoresis ribotyping KW - Clostridium difficile KW - MLST KW - MLVA KW - Moxifloxacin resistance SP - 344 EP - 351 JF - Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi JO - J Microbiol Immunol Infect VL - 51 IS - 3 N2 - PURPOSE: To characterise and compare twenty-eight Finnish Clostridium difficile RT027-like isolates, selected based on the presence of 18 bp deletion in the tcdC gene and toxin gene profile (A, B, binary), with eleven RT027 isolates from different Finnish geographical areas and time periods. METHODS: Twenty-eight C. difficile RT027-like isolates and 11 RT027 comparative strains were characterised by capillary-electrophoresis (CE) ribotyping, multi-locus variable tandem-repeats analysis (MLVA), multi-locus sequence typing (MLST), and sequencing of tcdC and gyrA gene fragments. Susceptibility to moxifloxacin was determined by E-test. RESULTS: Of 28 RT027-like isolates, seven RTs (016, 034, 075, 080, 153, 176 and 328), three WEBRIBO types (411, 475, AI-78) and three new profiles (F1-F3) were identified. MLVA revealed six clonal complexes (RTs 016, 027, 176 and F3). MLST showed eleven sequence types (1, 41, 47, 67, 95, 191,192, 223, 229, 264 and new ST). Twenty-two isolates (RTs 016, 080, 176, 328, F1, F2, F3 and WRTAI-78) carried Δ117 in the tcdC gene. Isolates of RTs 016, 027 and 176 were moxifloxacin resistant and harboured Thr82Ile in the GyrA. CONCLUSION: Our results show a high diversity within 28 Finnish RT027-like C. difficile isolates, with twelve CE-ribotyping profiles and eleven STs. MLVA revealed the regional spread of RTs 016, 027, 176 and F3. The presence of Δ117 in the tcdC gene in eight non-027 RTs highlights the importance of careful interpretation of the results from molecular systems targeting this site in the genome of C. difficile and the need of strain typing for epidemiological purposes. SN - 1995-9133 UR - https://www.unboundmedicine.com/medline/citation/28583353/The_recognition_and_characterisation_of_Finnish_Clostridium_difficile_isolates_resembling_PCR_ribotype_027_ DB - PRIME DP - Unbound Medicine ER -