Tags

Type your tag names separated by a space and hit enter

A diet rich in omega-3 fatty acids enhances expression of soluble epoxide hydrolase in murine brain.

Abstract

Several studies suggest that intake of omega-3 polyunsaturated fatty acids (n3-PUFA) beneficially influences cognitive function. However, effects on the adult brain are not clear. Little is known about the impact of dietary intervention on the fatty acid profile in adult brain, the modulation in the expression of enzymes involved in fatty acid biosynthesis and metabolism as well as changes in resulting oxylipins. These questions were addressed in the present study in two independent n3-PUFA feeding experiments in mice. Supplementation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA, 1% each in the diet) for 30days to adult NMRI and C57BL/6 mice led to a distinct shift in the brain PUFA pattern. While n3-PUFAs EPA, n3 docosapentaenoic acid and DHA were elevated, many n6-PUFAs were significantly decreased (except, e.g. C20:3 n6 which was increased). This shift in PUFAs was accompanied by immense differences in concentrations of oxidative metabolites derived from enzymatic conversion of PUFAs, esp. arachidonic acid whose products were uniformly decreased, and a modulation in the activity and expression pattern of delta-5 and delta-6 desaturases. In both mouse strains a remarkable increase in the soluble epoxide hydrolase (sEH) activity (decreased epoxy-FA concentrations and epoxy-FA to dihydroxy-FA-ratios) as well as sEH expression was observed. Taking the high biological activity of epoxy-FA, e.g. on blood flow and nociceptive signaling into account, this finding might be of relevance for the effects of n3-PUFAs in neurodegenerative diseases. On any account, our study suggests a new distinct regulation of brain PUFA and oxylipin pattern by supplementation of n3-PUFAs to adult rodents.

Authors+Show Affiliations

Institute for Food Toxicology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.Institute of Nutritional Sciences, Justus-Liebig-University, Wilhelmstr. 20, 35392 Giessen, Germany.Institute for Food Toxicology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.Institute of Nutritional Sciences, Justus-Liebig-University, Wilhelmstr. 20, 35392 Giessen, Germany.Institute for Food Toxicology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.Institute for Food Toxicology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.Medical Department, Division of Hepatology and Gastroenterology (including Metabolic Diseases), Charité University Medicine Berlin, Campus Virchow Klinikum, Berlin, Germany; Experimental and Clinical Research Centre, Charité University Medicine, Campus Buch, Berlin, Germany; Medical Department, Division of Gastroenterology, Oncology, Hematology, Rheumatology and Diabetes, Ruppiner Kliniken, Brandenburg Medical School, Neuruppin, Germany.Institute of Nutritional Sciences, Justus-Liebig-University, Wilhelmstr. 20, 35392 Giessen, Germany.Institute for Food Toxicology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany; Chair of Food Chemistry, Faculty of Mathematics and Natural Sciences, University of Wuppertal, Gauβstr. 20, 42119 Wuppertal, Germany. Electronic address: nils@schebb-web.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28583889

Citation

Ostermann, Annika Irmgard, et al. "A Diet Rich in Omega-3 Fatty Acids Enhances Expression of Soluble Epoxide Hydrolase in Murine Brain." Prostaglandins & Other Lipid Mediators, vol. 133, 2017, pp. 79-87.
Ostermann AI, Reutzel M, Hartung N, et al. A diet rich in omega-3 fatty acids enhances expression of soluble epoxide hydrolase in murine brain. Prostaglandins Other Lipid Mediat. 2017;133:79-87.
Ostermann, A. I., Reutzel, M., Hartung, N., Franke, N., Kutzner, L., Schoenfeld, K., ... Schebb, N. H. (2017). A diet rich in omega-3 fatty acids enhances expression of soluble epoxide hydrolase in murine brain. Prostaglandins & Other Lipid Mediators, 133, pp. 79-87. doi:10.1016/j.prostaglandins.2017.06.001.
Ostermann AI, et al. A Diet Rich in Omega-3 Fatty Acids Enhances Expression of Soluble Epoxide Hydrolase in Murine Brain. Prostaglandins Other Lipid Mediat. 2017;133:79-87. PubMed PMID: 28583889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A diet rich in omega-3 fatty acids enhances expression of soluble epoxide hydrolase in murine brain. AU - Ostermann,Annika Irmgard, AU - Reutzel,Martina, AU - Hartung,Nicole, AU - Franke,Nicole, AU - Kutzner,Laura, AU - Schoenfeld,Kirsten, AU - Weylandt,Karsten-Henrich, AU - Eckert,Gunter Peter, AU - Schebb,Nils Helge, Y1 - 2017/06/03/ PY - 2017/03/01/received PY - 2017/04/28/revised PY - 2017/06/01/accepted PY - 2017/6/7/pubmed PY - 2018/7/17/medline PY - 2017/6/7/entrez SP - 79 EP - 87 JF - Prostaglandins & other lipid mediators JO - Prostaglandins Other Lipid Mediat. VL - 133 N2 - Several studies suggest that intake of omega-3 polyunsaturated fatty acids (n3-PUFA) beneficially influences cognitive function. However, effects on the adult brain are not clear. Little is known about the impact of dietary intervention on the fatty acid profile in adult brain, the modulation in the expression of enzymes involved in fatty acid biosynthesis and metabolism as well as changes in resulting oxylipins. These questions were addressed in the present study in two independent n3-PUFA feeding experiments in mice. Supplementation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA, 1% each in the diet) for 30days to adult NMRI and C57BL/6 mice led to a distinct shift in the brain PUFA pattern. While n3-PUFAs EPA, n3 docosapentaenoic acid and DHA were elevated, many n6-PUFAs were significantly decreased (except, e.g. C20:3 n6 which was increased). This shift in PUFAs was accompanied by immense differences in concentrations of oxidative metabolites derived from enzymatic conversion of PUFAs, esp. arachidonic acid whose products were uniformly decreased, and a modulation in the activity and expression pattern of delta-5 and delta-6 desaturases. In both mouse strains a remarkable increase in the soluble epoxide hydrolase (sEH) activity (decreased epoxy-FA concentrations and epoxy-FA to dihydroxy-FA-ratios) as well as sEH expression was observed. Taking the high biological activity of epoxy-FA, e.g. on blood flow and nociceptive signaling into account, this finding might be of relevance for the effects of n3-PUFAs in neurodegenerative diseases. On any account, our study suggests a new distinct regulation of brain PUFA and oxylipin pattern by supplementation of n3-PUFAs to adult rodents. SN - 1098-8823 UR - https://www.unboundmedicine.com/medline/citation/28583889/A_diet_rich_in_omega_3_fatty_acids_enhances_expression_of_soluble_epoxide_hydrolase_in_murine_brain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1098-8823(17)30043-6 DB - PRIME DP - Unbound Medicine ER -