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Controlled water deficit during ripening affects proanthocyanidin synthesis, concentration and composition in Cabernet Sauvignon grape skins.
Plant Physiol Biochem. 2017 Aug; 117:34-41.PP

Abstract

The influence of controlled water deficit on the phenolic composition and gene expression of VvLAR2, VvMYBPA1, VvMYBPA2 and VvMYB4a in Cabernet Sauvignon grape skins throughout ripening was investigated. The assay was carried out on own-rooted Vitis vinifera plants cv. Cabernet Sauvignon in a commercial vineyard from veraison until commercial harvest. Three irrigation regimes were used from veraison until harvest with the following treatments: T1: 3.6 mm day-1; T2: 1.8 mm day-1 and T3: 0.3 mm day-1. The content of total phenols and total anthocyanins in grape skins increased during ripening, but water deficit did not produce differences among treatments in the total anthocyanin concentration. Proanthocyanidins (PAs) decreased throughout ripening, although approximately 25 days after veraison (DAV), their content slightly increased. This effect was more pronounced in the most restrictive treatment (T3). A similar pattern was observed in the transcript abundance of VvLAR2, VvMYBPA1 and VvMYB4a. PAs separation revealed differences in concentration but not in the proportion among fractions among the irrigation treatments. Additionally, controlled water deficit increased the mean degree of polymerization and the flavan-3-ol polymeric concentration in grape skins throughout ripening but with no effects on the extent of PAs galloylation. Our results suggest that the water status of Cabernet Sauvignon grapevines affects the gene expression for proteins involved in the synthesis of PAs, increasing their concentration and also their composition, with further evidence for the efficacy of a convenient, controlled water deficit strategy for grapevine cultivation.

Authors+Show Affiliations

Escuela de Agronomía, Pontificia Universidad Católica de Valparaíso, San Francisco s/n, La Palma, Casilla 4-D, Quillota, Chile. Electronic address: alejando.caceres@pucv.cl.CICYTEX-Technological Institute of Food and Agriculture-INTAEX (Government of Extremadura), Adolfo Suarez, s/n 06071, Badajoz, Spain.Universidad de Chile, Facultad de Ciencias Agronómicas, Santa Rosa 11315, La Pintana, Casilla 1004, Santiago, Chile.Centro Regional de Innovación Hortofrutícola de Valparaíso (Ceres), Pontificia Universidad Católica de Valparaíso, Av. San Francisco s/n, La Palma, Quillota, Chile.Universidad de Chile, Facultad de Ciencias Agronómicas, Santa Rosa 11315, La Pintana, Casilla 1004, Santiago, Chile.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28587991

Citation

Cáceres-Mella, Alejandro, et al. "Controlled Water Deficit During Ripening Affects Proanthocyanidin Synthesis, Concentration and Composition in Cabernet Sauvignon Grape Skins." Plant Physiology and Biochemistry : PPB, vol. 117, 2017, pp. 34-41.
Cáceres-Mella A, Talaverano MI, Villalobos-González L, et al. Controlled water deficit during ripening affects proanthocyanidin synthesis, concentration and composition in Cabernet Sauvignon grape skins. Plant Physiol Biochem. 2017;117:34-41.
Cáceres-Mella, A., Talaverano, M. I., Villalobos-González, L., Ribalta-Pizarro, C., & Pastenes, C. (2017). Controlled water deficit during ripening affects proanthocyanidin synthesis, concentration and composition in Cabernet Sauvignon grape skins. Plant Physiology and Biochemistry : PPB, 117, 34-41. https://doi.org/10.1016/j.plaphy.2017.05.015
Cáceres-Mella A, et al. Controlled Water Deficit During Ripening Affects Proanthocyanidin Synthesis, Concentration and Composition in Cabernet Sauvignon Grape Skins. Plant Physiol Biochem. 2017;117:34-41. PubMed PMID: 28587991.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Controlled water deficit during ripening affects proanthocyanidin synthesis, concentration and composition in Cabernet Sauvignon grape skins. AU - Cáceres-Mella,Alejandro, AU - Talaverano,M Inmaculada, AU - Villalobos-González,Luis, AU - Ribalta-Pizarro,Camila, AU - Pastenes,Claudio, Y1 - 2017/05/26/ PY - 2017/03/20/received PY - 2017/05/17/revised PY - 2017/05/25/accepted PY - 2017/6/8/pubmed PY - 2017/12/14/medline PY - 2017/6/8/entrez KW - Cabernet Sauvignon KW - Gene expression KW - Grape skins KW - Polymerization KW - Proanthocyanidins KW - Water deficit SP - 34 EP - 41 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 117 N2 - The influence of controlled water deficit on the phenolic composition and gene expression of VvLAR2, VvMYBPA1, VvMYBPA2 and VvMYB4a in Cabernet Sauvignon grape skins throughout ripening was investigated. The assay was carried out on own-rooted Vitis vinifera plants cv. Cabernet Sauvignon in a commercial vineyard from veraison until commercial harvest. Three irrigation regimes were used from veraison until harvest with the following treatments: T1: 3.6 mm day-1; T2: 1.8 mm day-1 and T3: 0.3 mm day-1. The content of total phenols and total anthocyanins in grape skins increased during ripening, but water deficit did not produce differences among treatments in the total anthocyanin concentration. Proanthocyanidins (PAs) decreased throughout ripening, although approximately 25 days after veraison (DAV), their content slightly increased. This effect was more pronounced in the most restrictive treatment (T3). A similar pattern was observed in the transcript abundance of VvLAR2, VvMYBPA1 and VvMYB4a. PAs separation revealed differences in concentration but not in the proportion among fractions among the irrigation treatments. Additionally, controlled water deficit increased the mean degree of polymerization and the flavan-3-ol polymeric concentration in grape skins throughout ripening but with no effects on the extent of PAs galloylation. Our results suggest that the water status of Cabernet Sauvignon grapevines affects the gene expression for proteins involved in the synthesis of PAs, increasing their concentration and also their composition, with further evidence for the efficacy of a convenient, controlled water deficit strategy for grapevine cultivation. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/28587991/Controlled_water_deficit_during_ripening_affects_proanthocyanidin_synthesis_concentration_and_composition_in_Cabernet_Sauvignon_grape_skins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(17)30172-9 DB - PRIME DP - Unbound Medicine ER -