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Evaluation of phenolic profile, enzyme inhibitory and antimicrobial activities of Nigella sativa L. seed extracts.
Bosn J Basic Med Sci. 2017 Nov 20; 17(4):286-294.BJ

Abstract

Black cumin (Nigella sativa L. [N.sativa]) seed extracts demonstrated numerous beneficial biological effects including, among others, antidiabetic, anticancer, immunomodulatory, antimicrobial, anti-inflammatory, antihypertensive, and antioxidant activity. To better understand the phytochemical composition of N. sativa seeds, methanol seed extracts were analyzed for phenolic acid and flavonoid content. Furthermore, we tested N. sativa methanol, n-hexane, and aqueous seed extracts for their inhibitory activity against butyrylcholinesterase (BChE) and catalase (CAT) as well as for antimicrobial activity against several bacterial and a yeast strains. The phenolic content of N. sativa was analyzed using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The inhibition of BChE was assessed by modified Ellman's method, and the inhibition of CAT was determined by monitoring hydrogen peroxide consumption. The extracts were tested against Bacillus subtilis, Staphylococcus aureus, Salmonella enterica, and Escherichia coli using the agar diffusion method. The UHPLC-MS/MS method allowed the identification and quantification of 23 phenolic compounds within 15 minutes. The major components found in N. sativa seed extract were sinapinic acid (7.22 ± 0.73 µg/mg) as a phenolic acid and kaempferol (11.74 ± 0.92 µg/mg) as a flavonoid. All extracts showed inhibitory activity against BChE, with methanol seed extract demonstrating the highest inhibitory activity (inhibitory concentration 50% [IC50] 79.11 ± 6.06 µg/ml). The methanol seed extract also showed strong inhibitory activity against CAT with an IC50 value of 6.61 ± 0.27 µg/ml. Finally, the methanol extract exhibited considerable inhibitory activity against the tested microbial strains. Overall, this is the first study to investigate the ability of black cumin seed extracts to inhibit CAT. Our results indicate that N. sativa seed can be considered as an effective inhibitor of CAT activity.

Authors+Show Affiliations

Department of Chemistry, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina. anela.topcagic@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28590231

Citation

Topcagic, Anela, et al. "Evaluation of Phenolic Profile, Enzyme Inhibitory and Antimicrobial Activities of Nigella Sativa L. Seed Extracts." Bosnian Journal of Basic Medical Sciences, vol. 17, no. 4, 2017, pp. 286-294.
Topcagic A, Cavar Zeljkovic S, Karalija E, et al. Evaluation of phenolic profile, enzyme inhibitory and antimicrobial activities of Nigella sativa L. seed extracts. Bosn J Basic Med Sci. 2017;17(4):286-294.
Topcagic, A., Cavar Zeljkovic, S., Karalija, E., Galijasevic, S., & Sofic, E. (2017). Evaluation of phenolic profile, enzyme inhibitory and antimicrobial activities of Nigella sativa L. seed extracts. Bosnian Journal of Basic Medical Sciences, 17(4), 286-294. https://doi.org/10.17305/bjbms.2017.2049
Topcagic A, et al. Evaluation of Phenolic Profile, Enzyme Inhibitory and Antimicrobial Activities of Nigella Sativa L. Seed Extracts. Bosn J Basic Med Sci. 2017 Nov 20;17(4):286-294. PubMed PMID: 28590231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of phenolic profile, enzyme inhibitory and antimicrobial activities of Nigella sativa L. seed extracts. AU - Topcagic,Anela, AU - Cavar Zeljkovic,Sanja, AU - Karalija,Erna, AU - Galijasevic,Semira, AU - Sofic,Emin, Y1 - 2017/11/20/ PY - 2017/03/16/received PY - 2017/04/26/accepted PY - 2017/04/24/revised PY - 2017/6/8/pubmed PY - 2018/7/28/medline PY - 2017/6/8/entrez SP - 286 EP - 294 JF - Bosnian journal of basic medical sciences JO - Bosn J Basic Med Sci VL - 17 IS - 4 N2 - Black cumin (Nigella sativa L. [N.sativa]) seed extracts demonstrated numerous beneficial biological effects including, among others, antidiabetic, anticancer, immunomodulatory, antimicrobial, anti-inflammatory, antihypertensive, and antioxidant activity. To better understand the phytochemical composition of N. sativa seeds, methanol seed extracts were analyzed for phenolic acid and flavonoid content. Furthermore, we tested N. sativa methanol, n-hexane, and aqueous seed extracts for their inhibitory activity against butyrylcholinesterase (BChE) and catalase (CAT) as well as for antimicrobial activity against several bacterial and a yeast strains. The phenolic content of N. sativa was analyzed using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The inhibition of BChE was assessed by modified Ellman's method, and the inhibition of CAT was determined by monitoring hydrogen peroxide consumption. The extracts were tested against Bacillus subtilis, Staphylococcus aureus, Salmonella enterica, and Escherichia coli using the agar diffusion method. The UHPLC-MS/MS method allowed the identification and quantification of 23 phenolic compounds within 15 minutes. The major components found in N. sativa seed extract were sinapinic acid (7.22 ± 0.73 µg/mg) as a phenolic acid and kaempferol (11.74 ± 0.92 µg/mg) as a flavonoid. All extracts showed inhibitory activity against BChE, with methanol seed extract demonstrating the highest inhibitory activity (inhibitory concentration 50% [IC50] 79.11 ± 6.06 µg/ml). The methanol seed extract also showed strong inhibitory activity against CAT with an IC50 value of 6.61 ± 0.27 µg/ml. Finally, the methanol extract exhibited considerable inhibitory activity against the tested microbial strains. Overall, this is the first study to investigate the ability of black cumin seed extracts to inhibit CAT. Our results indicate that N. sativa seed can be considered as an effective inhibitor of CAT activity. SN - 1840-4812 UR - https://www.unboundmedicine.com/medline/citation/28590231/Evaluation_of_phenolic_profile_enzyme_inhibitory_and_antimicrobial_activities_of_Nigella_sativa_L__seed_extracts_ L2 - https://doi.org/10.17305/bjbms.2017.2049 DB - PRIME DP - Unbound Medicine ER -