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Loci associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a core collection of spring wheat (Triticum aestivum).
PLoS One. 2017; 12(6):e0179087.Plos

Abstract

Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst) remains one of the most significant diseases of wheat worldwide. We investigated stripe rust resistance by genome-wide association analysis (GWAS) in 959 spring wheat accessions from the United States Department of Agriculture-Agricultural Research Service National Small Grains Collection, representing major global production environments. The panel was characterized for field resistance in multi-environment field trials and seedling resistance under greenhouse conditions. A genome-wide set of 5,619 informative SNP markers were used to examine the population structure, linkage disequilibrium and marker-trait associations in the germplasm panel. Based on model-based analysis of population structure and hierarchical Ward clustering algorithm, the accessions were clustered into two major subgroups. These subgroups were largely separated according to geographic origin and improvement status of the accessions. A significant correlation was observed between the population sub-clusters and response to stripe rust infection. We identified 11 and 7 genomic regions with significant associations with stripe rust resistance at adult plant and seedling stages, respectively, based on a false discovery rate multiple correction method. The regions harboring all, except three, of the QTL identified from the field and greenhouse studies overlap with positions of previously reported QTL. Further work should aim at validating the identified QTL using proper germplasm and populations to enhance their utility in marker assisted breeding.

Authors+Show Affiliations

Department of Crop and Soil Sciences, Washington State University, Pullman, Washington, United States of America.Department of Crop and Soil Sciences, Washington State University, Pullman, Washington, United States of America.Department of Crop and Soil Sciences, Washington State University, Pullman, Washington, United States of America.USDA-ARS, Wheat Health, Genetics, and Quality Research Unit, and Department of Plant Pathology, Washington State University, Pullman, Washington, United States of America.Department of Crop and Soil Sciences, Washington State University, Pullman, Washington, United States of America.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28591221

Citation

Muleta, Kebede T., et al. "Loci Associated With Resistance to Stripe Rust (Puccinia Striiformis F. Sp. Tritici) in a Core Collection of Spring Wheat (Triticum Aestivum)." PloS One, vol. 12, no. 6, 2017, pp. e0179087.
Muleta KT, Bulli P, Rynearson S, et al. Loci associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a core collection of spring wheat (Triticum aestivum). PLoS One. 2017;12(6):e0179087.
Muleta, K. T., Bulli, P., Rynearson, S., Chen, X., & Pumphrey, M. (2017). Loci associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a core collection of spring wheat (Triticum aestivum). PloS One, 12(6), e0179087. https://doi.org/10.1371/journal.pone.0179087
Muleta KT, et al. Loci Associated With Resistance to Stripe Rust (Puccinia Striiformis F. Sp. Tritici) in a Core Collection of Spring Wheat (Triticum Aestivum). PLoS One. 2017;12(6):e0179087. PubMed PMID: 28591221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Loci associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a core collection of spring wheat (Triticum aestivum). AU - Muleta,Kebede T, AU - Bulli,Peter, AU - Rynearson,Sheri, AU - Chen,Xianming, AU - Pumphrey,Michael, Y1 - 2017/06/07/ PY - 2017/01/24/received PY - 2017/05/23/accepted PY - 2017/6/8/entrez PY - 2017/6/8/pubmed PY - 2017/9/14/medline SP - e0179087 EP - e0179087 JF - PloS one JO - PLoS One VL - 12 IS - 6 N2 - Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst) remains one of the most significant diseases of wheat worldwide. We investigated stripe rust resistance by genome-wide association analysis (GWAS) in 959 spring wheat accessions from the United States Department of Agriculture-Agricultural Research Service National Small Grains Collection, representing major global production environments. The panel was characterized for field resistance in multi-environment field trials and seedling resistance under greenhouse conditions. A genome-wide set of 5,619 informative SNP markers were used to examine the population structure, linkage disequilibrium and marker-trait associations in the germplasm panel. Based on model-based analysis of population structure and hierarchical Ward clustering algorithm, the accessions were clustered into two major subgroups. These subgroups were largely separated according to geographic origin and improvement status of the accessions. A significant correlation was observed between the population sub-clusters and response to stripe rust infection. We identified 11 and 7 genomic regions with significant associations with stripe rust resistance at adult plant and seedling stages, respectively, based on a false discovery rate multiple correction method. The regions harboring all, except three, of the QTL identified from the field and greenhouse studies overlap with positions of previously reported QTL. Further work should aim at validating the identified QTL using proper germplasm and populations to enhance their utility in marker assisted breeding. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28591221/Loci_associated_with_resistance_to_stripe_rust__Puccinia_striiformis_f__sp__tritici__in_a_core_collection_of_spring_wheat__Triticum_aestivum__ DB - PRIME DP - Unbound Medicine ER -