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Pseudomonas aeruginosa quorum-sensing response in the absence of functional LasR and LasI proteins: the case of strain 148, a virulent dolphin isolate.
FEMS Microbiol Lett. 2017 07 03; 364(12)FM

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that presents a complex regulatory network called 'quorum-sensing', which is responsible for the transcription of genes coding for several traits implicated in its pathogenicity. Strain 148 is a dolphin isolate that has been shown to produce quorum-sensing-regulated virulence traits and to be virulent in a mouse model, despite the fact that it contains a 20-kbp deletion that eliminates from the chromosome the lasR gene and the lasI promoter. LasR is a key quorum-sensing transcriptional regulator that, when coupled with the autoinducer 3-oxo-dodecanoyl homoserine lactone (3O-C12-HSL) produced by LasI, activates transcription of genes coding for some virulence-associated traits such as elastase, lasI, rhlI and rhlR. RhlR is also a key quorum-sensing transcriptional regulator that, when interacting with the autoinducer butanoyl homoserine lactone (C4-HSL) that is produced by the synthase RhlI, activates the genes involved in the synthesis of some virulence-associated traits, as rhamnolipids and pyocyanin. We describe that in P. aeruginosa 148, the LasR/3O-C12-HSL-independent rhlR transcriptional activation is due to the release of the negative effect of Vfr (a CRP-ortholog) caused by the insertion of an IS element in vfr, and that rhlI transcription is driven from the rhlR promoter, forming the rhlR-I operon.

Authors+Show Affiliations

Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, México, D. F. México.Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, México, D. F. México.Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, México, D. F. México.Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, México, D. F. México.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28591849

Citation

Morales, Estefanía, et al. "Pseudomonas Aeruginosa Quorum-sensing Response in the Absence of Functional LasR and LasI Proteins: the Case of Strain 148, a Virulent Dolphin Isolate." FEMS Microbiology Letters, vol. 364, no. 12, 2017.
Morales E, González-Valdez A, Servín-González L, et al. Pseudomonas aeruginosa quorum-sensing response in the absence of functional LasR and LasI proteins: the case of strain 148, a virulent dolphin isolate. FEMS Microbiol Lett. 2017;364(12).
Morales, E., González-Valdez, A., Servín-González, L., & Soberón-Chávez, G. (2017). Pseudomonas aeruginosa quorum-sensing response in the absence of functional LasR and LasI proteins: the case of strain 148, a virulent dolphin isolate. FEMS Microbiology Letters, 364(12). https://doi.org/10.1093/femsle/fnx119
Morales E, et al. Pseudomonas Aeruginosa Quorum-sensing Response in the Absence of Functional LasR and LasI Proteins: the Case of Strain 148, a Virulent Dolphin Isolate. FEMS Microbiol Lett. 2017 07 3;364(12) PubMed PMID: 28591849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pseudomonas aeruginosa quorum-sensing response in the absence of functional LasR and LasI proteins: the case of strain 148, a virulent dolphin isolate. AU - Morales,Estefanía, AU - González-Valdez,Abigail, AU - Servín-González,Luis, AU - Soberón-Chávez,Gloria, PY - 2017/03/29/received PY - 2017/06/06/accepted PY - 2017/6/8/pubmed PY - 2018/1/23/medline PY - 2017/6/8/entrez KW - LasR deficiency KW - atypical Pseudomonas aeruginosa strains KW - quorum sensing JF - FEMS microbiology letters JO - FEMS Microbiol. Lett. VL - 364 IS - 12 N2 - Pseudomonas aeruginosa is an opportunistic pathogen that presents a complex regulatory network called 'quorum-sensing', which is responsible for the transcription of genes coding for several traits implicated in its pathogenicity. Strain 148 is a dolphin isolate that has been shown to produce quorum-sensing-regulated virulence traits and to be virulent in a mouse model, despite the fact that it contains a 20-kbp deletion that eliminates from the chromosome the lasR gene and the lasI promoter. LasR is a key quorum-sensing transcriptional regulator that, when coupled with the autoinducer 3-oxo-dodecanoyl homoserine lactone (3O-C12-HSL) produced by LasI, activates transcription of genes coding for some virulence-associated traits such as elastase, lasI, rhlI and rhlR. RhlR is also a key quorum-sensing transcriptional regulator that, when interacting with the autoinducer butanoyl homoserine lactone (C4-HSL) that is produced by the synthase RhlI, activates the genes involved in the synthesis of some virulence-associated traits, as rhamnolipids and pyocyanin. We describe that in P. aeruginosa 148, the LasR/3O-C12-HSL-independent rhlR transcriptional activation is due to the release of the negative effect of Vfr (a CRP-ortholog) caused by the insertion of an IS element in vfr, and that rhlI transcription is driven from the rhlR promoter, forming the rhlR-I operon. SN - 1574-6968 UR - https://www.unboundmedicine.com/medline/citation/28591849/Pseudomonas_aeruginosa_quorum_sensing_response_in_the_absence_of_functional_LasR_and_LasI_proteins:_the_case_of_strain_148_a_virulent_dolphin_isolate_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1093/femsle/fnx119 DB - PRIME DP - Unbound Medicine ER -