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Exploring the putative role of TRPV1 -dependent CGRP release in remote hind preconditioning-induced cardioprotection.
Cardiovasc Ther. 2017 Oct; 35(5)CT

Abstract

BACKGROUND

Remote ischemic preconditioning (RIPC) is a phenomenon whereby transient nonlethal ischemia and reperfusion episodes confer protection against prolonged ischemia reperfusion-induced injury. However, the underlying intracellular signaling has not been extensively explored.

OBJECTIVE

This study aimed to inspect the putative involvement of TRPV1 -dependent CGRP release in mediating remote hind limb preconditioning-induced cardioprotection.

METHODS

In this study, remote hind limb preconditioning stimulus was delivered (four consecutive episodes of 5 minutes of ischemia reperfusion) using a blood pressure cuff tied at the inguinal level of the rat. The isolated rat hearts were perfused on the Langendorff's system and were subjected to 30-minutes global ischemia and 120-minutes reperfusion. Prolonged ischemia and subsequent reperfusion led to myocardial injury that was evaluated in terms of infarct size, LDH release, CK release, LVDP, +dp/dtmax , -dp/dtmin , and coronary flow rate. The pharmacological agents used in this study included capsaicin as TRPV1 channel activator, sumatriptan and CGRP8-37 as CGRP blockers.

RESULTS

Remote hind limb and capsaicin preconditioning (10 mg/kg-1) significantly reduced the infarct size, LDH release, CK release and significantly improved LVDP, +dp/dtmax , -dp/dtmin , and coronary flow rate. However, remote hind limb and capsaicin preconditioning-induced cardioprotective effects were remarkably reduced in the presence of sumatriptan (8 mg/kg-1) and CGRP8-37 (1 mg/kg-1).

CONCLUSION

This indicates that remote hind limb preconditioning stimulus probably activates TRPV1 channels which subsequently induces CGRP release to produce cardioprotective effects.

Authors+Show Affiliations

Department of Pharmaceutical Sciences and Drug Research, Punjabi University Patiala, Patiala, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University Patiala, Patiala, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28599085

Citation

Randhawa, Puneet Kaur, and Amteshwar Singh Jaggi. "Exploring the Putative Role of TRPV1 -dependent CGRP Release in Remote Hind Preconditioning-induced Cardioprotection." Cardiovascular Therapeutics, vol. 35, no. 5, 2017.
Randhawa PK, Jaggi AS. Exploring the putative role of TRPV1 -dependent CGRP release in remote hind preconditioning-induced cardioprotection. Cardiovasc Ther. 2017;35(5).
Randhawa, P. K., & Jaggi, A. S. (2017). Exploring the putative role of TRPV1 -dependent CGRP release in remote hind preconditioning-induced cardioprotection. Cardiovascular Therapeutics, 35(5). https://doi.org/10.1111/1755-5922.12276
Randhawa PK, Jaggi AS. Exploring the Putative Role of TRPV1 -dependent CGRP Release in Remote Hind Preconditioning-induced Cardioprotection. Cardiovasc Ther. 2017;35(5) PubMed PMID: 28599085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exploring the putative role of TRPV1 -dependent CGRP release in remote hind preconditioning-induced cardioprotection. AU - Randhawa,Puneet Kaur, AU - Jaggi,Amteshwar Singh, PY - 2017/03/22/received PY - 2017/05/18/revised PY - 2017/06/03/accepted PY - 2017/6/10/pubmed PY - 2018/5/22/medline PY - 2017/6/10/entrez KW - CGRP KW - Capsaicin KW - Cardioprotection KW - Ischemia KW - Remote preconditioning KW - TRPV1 channels JF - Cardiovascular therapeutics JO - Cardiovasc Ther VL - 35 IS - 5 N2 - BACKGROUND: Remote ischemic preconditioning (RIPC) is a phenomenon whereby transient nonlethal ischemia and reperfusion episodes confer protection against prolonged ischemia reperfusion-induced injury. However, the underlying intracellular signaling has not been extensively explored. OBJECTIVE: This study aimed to inspect the putative involvement of TRPV1 -dependent CGRP release in mediating remote hind limb preconditioning-induced cardioprotection. METHODS: In this study, remote hind limb preconditioning stimulus was delivered (four consecutive episodes of 5 minutes of ischemia reperfusion) using a blood pressure cuff tied at the inguinal level of the rat. The isolated rat hearts were perfused on the Langendorff's system and were subjected to 30-minutes global ischemia and 120-minutes reperfusion. Prolonged ischemia and subsequent reperfusion led to myocardial injury that was evaluated in terms of infarct size, LDH release, CK release, LVDP, +dp/dtmax , -dp/dtmin , and coronary flow rate. The pharmacological agents used in this study included capsaicin as TRPV1 channel activator, sumatriptan and CGRP8-37 as CGRP blockers. RESULTS: Remote hind limb and capsaicin preconditioning (10 mg/kg-1) significantly reduced the infarct size, LDH release, CK release and significantly improved LVDP, +dp/dtmax , -dp/dtmin , and coronary flow rate. However, remote hind limb and capsaicin preconditioning-induced cardioprotective effects were remarkably reduced in the presence of sumatriptan (8 mg/kg-1) and CGRP8-37 (1 mg/kg-1). CONCLUSION: This indicates that remote hind limb preconditioning stimulus probably activates TRPV1 channels which subsequently induces CGRP release to produce cardioprotective effects. SN - 1755-5922 UR - https://www.unboundmedicine.com/medline/citation/28599085/Exploring_the_putative_role_of_TRPV1__dependent_CGRP_release_in_remote_hind_preconditioning_induced_cardioprotection_ L2 - https://doi.org/10.1111/1755-5922.12276 DB - PRIME DP - Unbound Medicine ER -