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A needle-type glucose biosensor based on PANI nanofibers and PU/E-PU membrane for long-term invasive continuous monitoring.
Biosens Bioelectron. 2017 Nov 15; 97:196-202.BB

Abstract

A minimally invasive glucose biosensor capable of continuous monitoring of subcutaneous glucose has been developed in this study. This sensor was prepared using electropolymerized conductive polymer polyaniline (PANI) nanofibers as an enzyme immobilization material and polyurethane (PU)/epoxy-enhanced polyurethane (E-PU) bilayer coating as a protective membrane. The sensor showed almost the same sensitivity (63nA/mM) and linearity (0-20mM with the correlation coefficient r2 of 0.9997) in both PBS and bovine serum tests. When stored in 37°C bovine serum, the sensor's sensitivity gradually increased about 30% of the initial value within the first 13 days and then remained stable for the rest of the study period of 53 days. In vivo implantation experiments using mice models showed real-time response to the variation of blood glucose with an average signal delay of about 8min. Continuous monitoring showed that the sensor response increased for the first 12 days and then entered a stable period for 14 days. The sensor's baseline (530±10nA) and the total response to 1ml 50% dextrose injection were almost the same (267±15nA) in the stable period. The in vivo stable performances indicated that the sensor could be used as an implantable device for long-term invasive monitoring of blood glucose.

Authors+Show Affiliations

College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou, China.Biosensor National Special Laboratory, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China.Biosensor National Special Laboratory, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China.Biosensor National Special Laboratory, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China.Biosensor National Special Laboratory, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China.Biosensor National Special Laboratory, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China; State Key Laboratory of CAD & CG, Zhejiang University, Hangzhou, China. Electronic address: yexuesong@zju.edu.cn.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

28599179

Citation

Fang, Lu, et al. "A Needle-type Glucose Biosensor Based On PANI Nanofibers and PU/E-PU Membrane for Long-term Invasive Continuous Monitoring." Biosensors & Bioelectronics, vol. 97, 2017, pp. 196-202.
Fang L, Liang B, Yang G, et al. A needle-type glucose biosensor based on PANI nanofibers and PU/E-PU membrane for long-term invasive continuous monitoring. Biosens Bioelectron. 2017;97:196-202.
Fang, L., Liang, B., Yang, G., Hu, Y., Zhu, Q., & Ye, X. (2017). A needle-type glucose biosensor based on PANI nanofibers and PU/E-PU membrane for long-term invasive continuous monitoring. Biosensors & Bioelectronics, 97, 196-202. https://doi.org/10.1016/j.bios.2017.04.043
Fang L, et al. A Needle-type Glucose Biosensor Based On PANI Nanofibers and PU/E-PU Membrane for Long-term Invasive Continuous Monitoring. Biosens Bioelectron. 2017 Nov 15;97:196-202. PubMed PMID: 28599179.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A needle-type glucose biosensor based on PANI nanofibers and PU/E-PU membrane for long-term invasive continuous monitoring. AU - Fang,Lu, AU - Liang,Bo, AU - Yang,Guang, AU - Hu,Yichuan, AU - Zhu,Qin, AU - Ye,Xuesong, Y1 - 2017/04/28/ PY - 2017/02/24/received PY - 2017/04/20/revised PY - 2017/04/27/accepted PY - 2017/6/10/pubmed PY - 2018/5/1/medline PY - 2017/6/10/entrez KW - Continuous monitoring KW - Epoxy-enhanced polyurethane KW - Glucose sensor KW - Invasive KW - Polyaniline SP - 196 EP - 202 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 97 N2 - A minimally invasive glucose biosensor capable of continuous monitoring of subcutaneous glucose has been developed in this study. This sensor was prepared using electropolymerized conductive polymer polyaniline (PANI) nanofibers as an enzyme immobilization material and polyurethane (PU)/epoxy-enhanced polyurethane (E-PU) bilayer coating as a protective membrane. The sensor showed almost the same sensitivity (63nA/mM) and linearity (0-20mM with the correlation coefficient r2 of 0.9997) in both PBS and bovine serum tests. When stored in 37°C bovine serum, the sensor's sensitivity gradually increased about 30% of the initial value within the first 13 days and then remained stable for the rest of the study period of 53 days. In vivo implantation experiments using mice models showed real-time response to the variation of blood glucose with an average signal delay of about 8min. Continuous monitoring showed that the sensor response increased for the first 12 days and then entered a stable period for 14 days. The sensor's baseline (530±10nA) and the total response to 1ml 50% dextrose injection were almost the same (267±15nA) in the stable period. The in vivo stable performances indicated that the sensor could be used as an implantable device for long-term invasive monitoring of blood glucose. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/28599179/A_needle_type_glucose_biosensor_based_on_PANI_nanofibers_and_PU/E_PU_membrane_for_long_term_invasive_continuous_monitoring_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(17)30294-4 DB - PRIME DP - Unbound Medicine ER -