Tags

Type your tag names separated by a space and hit enter

Schistosoma mansoni, S. japonicum, and S. haematobium: permeability to acidic amino acids and effect of separated and unseparated adults.
Exp Parasitol 1985; 59(3):355-63EP

Abstract

Permeability of the tegument of male and female Schistosoma mansoni was measured in vitro and a comparison was made between copulating and separated worms. In unpaired (separated) schistosomes, a carrier-mediated (selective) transport system for acidic amino acids was demonstrated. Males and females exhibited similar uptake rates for aspartate and glutamate. Half-saturation constants for aspartate (males, 0.035 +/- 0.008 mM; females, 0.026 +/- 0.006 mM) and glutamate (males, 0.010 +/- 0.007 mM; females, 0.015 +/- 0.004 mM) were determined for separated worms only. Time-course studies provided estimates of aspartate influx rates in males (7.3 pmol min-1 worm-1) and females (2.3 pmol min-1 worm-1). The most dramatic observation, however, was that, in copula, neither male nor female schistosomes took up acidic amino acids, but may have excluded these compounds. Thus, this ouabain-insensitive, mediated mechanism was operational only when the worms were unmated. In S. japonicum, no uptake of glutamate was observed in either mated or separated males and females. In S. haematobium, saturable uptake of aspartate was apparent in both mated and unmated males and females, indicating that species-specific differences in uptake of acidic amino acids existed. These studies indicate the need for cautious interpretation of data obtained from in vitro analyses of separated male and female mansonian schistosomes, and that such conditions may not reflect in vivo or in copula function.

Authors

No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2860003

Citation

Cornford, E M.. "Schistosoma Mansoni, S. Japonicum, and S. Haematobium: Permeability to Acidic Amino Acids and Effect of Separated and Unseparated Adults." Experimental Parasitology, vol. 59, no. 3, 1985, pp. 355-63.
Cornford EM. Schistosoma mansoni, S. japonicum, and S. haematobium: permeability to acidic amino acids and effect of separated and unseparated adults. Exp Parasitol. 1985;59(3):355-63.
Cornford, E. M. (1985). Schistosoma mansoni, S. japonicum, and S. haematobium: permeability to acidic amino acids and effect of separated and unseparated adults. Experimental Parasitology, 59(3), pp. 355-63.
Cornford EM. Schistosoma Mansoni, S. Japonicum, and S. Haematobium: Permeability to Acidic Amino Acids and Effect of Separated and Unseparated Adults. Exp Parasitol. 1985;59(3):355-63. PubMed PMID: 2860003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Schistosoma mansoni, S. japonicum, and S. haematobium: permeability to acidic amino acids and effect of separated and unseparated adults. A1 - Cornford,E M, PY - 1985/6/1/pubmed PY - 1985/6/1/medline PY - 1985/6/1/entrez SP - 355 EP - 63 JF - Experimental parasitology JO - Exp. Parasitol. VL - 59 IS - 3 N2 - Permeability of the tegument of male and female Schistosoma mansoni was measured in vitro and a comparison was made between copulating and separated worms. In unpaired (separated) schistosomes, a carrier-mediated (selective) transport system for acidic amino acids was demonstrated. Males and females exhibited similar uptake rates for aspartate and glutamate. Half-saturation constants for aspartate (males, 0.035 +/- 0.008 mM; females, 0.026 +/- 0.006 mM) and glutamate (males, 0.010 +/- 0.007 mM; females, 0.015 +/- 0.004 mM) were determined for separated worms only. Time-course studies provided estimates of aspartate influx rates in males (7.3 pmol min-1 worm-1) and females (2.3 pmol min-1 worm-1). The most dramatic observation, however, was that, in copula, neither male nor female schistosomes took up acidic amino acids, but may have excluded these compounds. Thus, this ouabain-insensitive, mediated mechanism was operational only when the worms were unmated. In S. japonicum, no uptake of glutamate was observed in either mated or separated males and females. In S. haematobium, saturable uptake of aspartate was apparent in both mated and unmated males and females, indicating that species-specific differences in uptake of acidic amino acids existed. These studies indicate the need for cautious interpretation of data obtained from in vitro analyses of separated male and female mansonian schistosomes, and that such conditions may not reflect in vivo or in copula function. SN - 0014-4894 UR - https://www.unboundmedicine.com/medline/citation/2860003/Schistosoma_mansoni_S__japonicum_and_S__haematobium:_permeability_to_acidic_amino_acids_and_effect_of_separated_and_unseparated_adults_ DB - PRIME DP - Unbound Medicine ER -