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Molecular epidemiology and antibiotic resistance of methicillin-resistant Staphylococcus aureus circulating in the Russian Federation.
Infect Genet Evol 2017; 53:189-194IG

Abstract

The aim of this study was to investigate the patterns of antimicrobial resistance and molecular features of methicillin-resistant Staphylococcus aureus (MRSA) isolates in Russia. Isolates recovered from hospital patients (n=480), healthy medical personnel (n=25), and healthy carriers (n=13) were included in the study. Hospital-acquired MRSA (HA-MRSA) demonstrated high resistance to ciprofloxacin, gentamicin, and chloramphenicol (76%-92%), moderate - to tetracycline, erythromycin, clindamycin, and rifampicin (38%-54%), and low - to fusidic acid, co-trimoxazole, mupirocin, and daptomycin (2%-7%). Elevated MIC (2.0μg/ml) of vancomycin was detected in 26% of isolates. All isolates were susceptible to linezolid and tigecycline. Multilocus sequence typing (MLST) revealed that CC8 isolates (ST8+ST239) constituted 83.1% of HA-MRSA and that this genetic lineage dominated in all regions from Krasnoyarsk to Saint Petersburg. A local ST239 variant harboring the tst gene (ST239Kras) was detected in Krasnoyarsk. The other HA-MRSA isolates belonged to clonal complex 5 (CC5) (21 isolates, 12.2%) and CC22 (2, 1.2%). The majority of CC5 isolates were affiliated with sequence type 228 (ST228) and were characterized with decreased susceptibility to ceftaroline (MIC=2μg/ml). We also detected, for the first time in Russia, livestock-associated MRSA (LA-MRSA) from clusters CC398 and CC97 in humans. Among the 2053 healthy persons screened for nasal carriage of S. aureus, the bacteria were isolated from 426 (21%); among them, 13 carried isolates identified as community-associated MRSA (CA-MRSA). Eleven of 13 CA-MRSA isolates belonged to ST22 (spa types t223, t3243, and t3689; SCCmec types IVa and IVc, agr type I, tst-positive) and were similar to the EMRSA-15/Middle Eastern variant (Gaza strain).

Authors+Show Affiliations

Scientific Research Institute of Children's Infections, Saint Petersburg, Russia.National Agency for Clinical Pharmacology and Pharmacy, Moscow, Russia.Scientific Research Institute of Children's Infections, Saint Petersburg, Russia.N. F. Gamaleya Federal Research Centre of Epidemiology and Microbiology, Moscow, Russia.State Medical University named after Professor V.F. Vojno-Yasenetsky, Krasnoyarsk, Russia.Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan; International Medical Education and Research Centre, Niigata, Japan.Scientific Research Institute of Children's Infections, Saint Petersburg, Russia; North-western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia.National Agency for Clinical Pharmacology and Pharmacy, Moscow, Russia.Scientific Research Institute of Children's Infections, Saint Petersburg, Russia; North-western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia. Electronic address: sidorserg@niidi.ru.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28600216

Citation

Gostev, Vladimir, et al. "Molecular Epidemiology and Antibiotic Resistance of Methicillin-resistant Staphylococcus Aureus Circulating in the Russian Federation." Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, vol. 53, 2017, pp. 189-194.
Gostev V, Kruglov A, Kalinogorskaya O, et al. Molecular epidemiology and antibiotic resistance of methicillin-resistant Staphylococcus aureus circulating in the Russian Federation. Infect Genet Evol. 2017;53:189-194.
Gostev, V., Kruglov, A., Kalinogorskaya, O., Dmitrenko, O., Khokhlova, O., Yamamoto, T., ... Sidorenko, S. (2017). Molecular epidemiology and antibiotic resistance of methicillin-resistant Staphylococcus aureus circulating in the Russian Federation. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 53, pp. 189-194. doi:10.1016/j.meegid.2017.06.006.
Gostev V, et al. Molecular Epidemiology and Antibiotic Resistance of Methicillin-resistant Staphylococcus Aureus Circulating in the Russian Federation. Infect Genet Evol. 2017;53:189-194. PubMed PMID: 28600216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology and antibiotic resistance of methicillin-resistant Staphylococcus aureus circulating in the Russian Federation. AU - Gostev,Vladimir, AU - Kruglov,Alexander, AU - Kalinogorskaya,Olga, AU - Dmitrenko,Olga, AU - Khokhlova,Olga, AU - Yamamoto,Tatsuo, AU - Lobzin,Yuri, AU - Ryabchenko,Irina, AU - Sidorenko,Sergey, Y1 - 2017/06/07/ PY - 2016/10/14/received PY - 2017/05/17/revised PY - 2017/06/06/accepted PY - 2017/6/11/pubmed PY - 2018/3/30/medline PY - 2017/6/11/entrez KW - CA-MRSA, multidrug resistance KW - HA-MRSA KW - Multi-locus sequence typing SP - 189 EP - 194 JF - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JO - Infect. Genet. Evol. VL - 53 N2 - The aim of this study was to investigate the patterns of antimicrobial resistance and molecular features of methicillin-resistant Staphylococcus aureus (MRSA) isolates in Russia. Isolates recovered from hospital patients (n=480), healthy medical personnel (n=25), and healthy carriers (n=13) were included in the study. Hospital-acquired MRSA (HA-MRSA) demonstrated high resistance to ciprofloxacin, gentamicin, and chloramphenicol (76%-92%), moderate - to tetracycline, erythromycin, clindamycin, and rifampicin (38%-54%), and low - to fusidic acid, co-trimoxazole, mupirocin, and daptomycin (2%-7%). Elevated MIC (2.0μg/ml) of vancomycin was detected in 26% of isolates. All isolates were susceptible to linezolid and tigecycline. Multilocus sequence typing (MLST) revealed that CC8 isolates (ST8+ST239) constituted 83.1% of HA-MRSA and that this genetic lineage dominated in all regions from Krasnoyarsk to Saint Petersburg. A local ST239 variant harboring the tst gene (ST239Kras) was detected in Krasnoyarsk. The other HA-MRSA isolates belonged to clonal complex 5 (CC5) (21 isolates, 12.2%) and CC22 (2, 1.2%). The majority of CC5 isolates were affiliated with sequence type 228 (ST228) and were characterized with decreased susceptibility to ceftaroline (MIC=2μg/ml). We also detected, for the first time in Russia, livestock-associated MRSA (LA-MRSA) from clusters CC398 and CC97 in humans. Among the 2053 healthy persons screened for nasal carriage of S. aureus, the bacteria were isolated from 426 (21%); among them, 13 carried isolates identified as community-associated MRSA (CA-MRSA). Eleven of 13 CA-MRSA isolates belonged to ST22 (spa types t223, t3243, and t3689; SCCmec types IVa and IVc, agr type I, tst-positive) and were similar to the EMRSA-15/Middle Eastern variant (Gaza strain). SN - 1567-7257 UR - https://www.unboundmedicine.com/medline/citation/28600216/Molecular_epidemiology_and_antibiotic_resistance_of_methicillin_resistant_Staphylococcus_aureus_circulating_in_the_Russian_Federation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-1348(17)30193-4 DB - PRIME DP - Unbound Medicine ER -