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Expression of GPD1 and SIP18 genes during rehydration in active dry industrial Saccharomyces cerevisiae cider-making yeast strains (ADY).
Acta Biochim Pol. 2017; 64(2):287-294.AB

Abstract

In this study we determined the influence of different sugar concentration in media, time of rehydration and type of strain on relative expression level of GPD1 and SIP18 genes of active dry cider-making yeast strains, followed by the assessment of the impact of rehydration on the fermentation process. High expression of SIP18 at the beginning of rehydration was shown to be due to high transcription of the gene during the drying process. High sugar concentrations of media initiated transcription of the GPD1 gene and triggered the cellular glycerol biosynthesis pathway in examined strains. Rehydration time and type of strain showed to have no statistically significant impact on the course of the fermentation; RT qPCR results depended mainly on the time of rehydration and sugar concentration of the medium. This is the first attempt to confront rehydration time and molecular mechanisms acting upon rehydration with the course of the fermentation process.

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Authors+Show Affiliations

Goncerzewicz A
Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Microbiology, Culture Collection of Industrial Microorganisms, Warsaw, Poland.
Kamińska-Wojteczek K
Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Microbiology, Culture Collection of Industrial Microorganisms, Warsaw, Poland.
Młynarczyk I
Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Microbiology, Culture Collection of Industrial Microorganisms, Warsaw, Poland.
Misiewicz A
Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Microbiology, Culture Collection of Industrial Microorganisms, Warsaw, Poland.

MeSH

Apoptosis Regulatory ProteinsBiosynthetic PathwaysFermentationFood MicrobiologyGene Expression Regulation, FungalGlycerolGlycerol-3-Phosphate Dehydrogenase (NAD+)Saccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28600909

Citation

Goncerzewicz, Anna, et al. "Expression of GPD1 and SIP18 Genes During Rehydration in Active Dry Industrial Saccharomyces Cerevisiae Cider-making Yeast Strains (ADY)." Acta Biochimica Polonica, vol. 64, no. 2, 2017, pp. 287-294.
Goncerzewicz A, Kamińska-Wojteczek K, Młynarczyk I, et al. Expression of GPD1 and SIP18 genes during rehydration in active dry industrial Saccharomyces cerevisiae cider-making yeast strains (ADY). Acta Biochim Pol. 2017;64(2):287-294.
Goncerzewicz, A., Kamińska-Wojteczek, K., Młynarczyk, I., & Misiewicz, A. (2017). Expression of GPD1 and SIP18 genes during rehydration in active dry industrial Saccharomyces cerevisiae cider-making yeast strains (ADY). Acta Biochimica Polonica, 64(2), 287-294. https://doi.org/10.18388/abp.2016_1404
Goncerzewicz A, et al. Expression of GPD1 and SIP18 Genes During Rehydration in Active Dry Industrial Saccharomyces Cerevisiae Cider-making Yeast Strains (ADY). Acta Biochim Pol. 2017;64(2):287-294. PubMed PMID: 28600909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of GPD1 and SIP18 genes during rehydration in active dry industrial Saccharomyces cerevisiae cider-making yeast strains (ADY). AU - Goncerzewicz,Anna, AU - Kamińska-Wojteczek,Karolina, AU - Młynarczyk,Izabella, AU - Misiewicz,Anna, Y1 - 2017/06/09/ PY - 2016/08/04/received PY - 2017/02/22/revised PY - 2017/02/25/accepted PY - 2017/6/11/pubmed PY - 2017/8/12/medline PY - 2017/6/11/entrez KW - fermentation biotechnology KW - gene expression KW - wine KW - yeast physiology KW - yeasts SP - 287 EP - 294 JF - Acta biochimica Polonica JO - Acta Biochim Pol VL - 64 IS - 2 N2 - In this study we determined the influence of different sugar concentration in media, time of rehydration and type of strain on relative expression level of GPD1 and SIP18 genes of active dry cider-making yeast strains, followed by the assessment of the impact of rehydration on the fermentation process. High expression of SIP18 at the beginning of rehydration was shown to be due to high transcription of the gene during the drying process. High sugar concentrations of media initiated transcription of the GPD1 gene and triggered the cellular glycerol biosynthesis pathway in examined strains. Rehydration time and type of strain showed to have no statistically significant impact on the course of the fermentation; RT qPCR results depended mainly on the time of rehydration and sugar concentration of the medium. This is the first attempt to confront rehydration time and molecular mechanisms acting upon rehydration with the course of the fermentation process. SN - 1734-154X UR - https://www.unboundmedicine.com/medline/citation/28600909/Expression_of_GPD1_and_SIP18_genes_during_rehydration_in_active_dry_industrial_Saccharomyces_cerevisiae_cider_making_yeast_strains__ADY__ L2 - http://ojs.ptbioch.edu.pl/index.php/abp/article/view/1404 DB - PRIME DP - Unbound Medicine ER -