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Dual signal amplification strategy of Au nanopaticles/ZnO nanorods hybridized reduced graphene nanosheet and multienzyme functionalized Au@ZnO composites for ultrasensitive electrochemical detection of tumor biomarker.
Biosens Bioelectron. 2017 Nov 15; 97:218-225.BB

Abstract

Herein, a dual signal amplification strategy was employed in fabricating ultrasensitive electrochemical immunosensor for alpha fetoprotein (AFP) detection, which was realized by utilizing of ZnO nanorods/Au nanopaticles hybridized reduced graphene nanosheet (Au/ZnO/RGO) and horseradish-peroxidase (HRP) bioconjugated detection antibody (Ab2) functionalized Au@ZnO (Ab2/HRP-Au@ZnO). During the fabrication of the immunosensor, a new kind of multiple-head surfactants CxN3 with different alkyl chain length played important roles such as acting as the surfactants of Au/ZnO/RGO and the reductant agents of Au@ZnO composite. Due to the good adsorption property and large surface area of Au/ZnO/RGO, plenty of the capture antibodies (Ab1) were immobilized on the electrode surface, and trace AFP was sensitively monitored. Furthermore, Ab2/HRP-Au@ZnO exhibited high affinity interaction with AFP through "sandwich" immunoreactions, along with the peroxidase-like catalytic activity of Au@ZnO, leading to a further enhancement in the sensitivity of the proposed immunosensor. The successful synthesis of the nanomaterials was characterized through a serious of techniques including Raman, XRD, FT-IR, SEM and UV-vis. Under the optimal conditions, two linear ranges of 0.02-10,000 and 10,000-100,000pgmL-1 AFP with a lower detection limit of 0.01pg mL-1 (S/N=3) was obtained. Especially, the proposed AFP immunosensor can be applied to detect human serum samples with satisfactory results, indicating a potential application in clinical monitoring of tumor biomarkers.

Authors+Show Affiliations

School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China. Electronic address: hongzhao@ucas.ac.cn.National Center for Nanoscience and Technology of China, Beijing 100080, China. Electronic address: bruseren@nanoctr.cn.School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China. Electronic address: lizengxi@ucas.ac.cn.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

28600990

Citation

Fang, Xian, et al. "Dual Signal Amplification Strategy of Au nanopaticles/ZnO Nanorods Hybridized Reduced Graphene Nanosheet and Multienzyme Functionalized Au@ZnO Composites for Ultrasensitive Electrochemical Detection of Tumor Biomarker." Biosensors & Bioelectronics, vol. 97, 2017, pp. 218-225.
Fang X, Liu J, Wang J, et al. Dual signal amplification strategy of Au nanopaticles/ZnO nanorods hybridized reduced graphene nanosheet and multienzyme functionalized Au@ZnO composites for ultrasensitive electrochemical detection of tumor biomarker. Biosens Bioelectron. 2017;97:218-225.
Fang, X., Liu, J., Wang, J., Zhao, H., Ren, H., & Li, Z. (2017). Dual signal amplification strategy of Au nanopaticles/ZnO nanorods hybridized reduced graphene nanosheet and multienzyme functionalized Au@ZnO composites for ultrasensitive electrochemical detection of tumor biomarker. Biosensors & Bioelectronics, 97, 218-225. https://doi.org/10.1016/j.bios.2017.05.055
Fang X, et al. Dual Signal Amplification Strategy of Au nanopaticles/ZnO Nanorods Hybridized Reduced Graphene Nanosheet and Multienzyme Functionalized Au@ZnO Composites for Ultrasensitive Electrochemical Detection of Tumor Biomarker. Biosens Bioelectron. 2017 Nov 15;97:218-225. PubMed PMID: 28600990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual signal amplification strategy of Au nanopaticles/ZnO nanorods hybridized reduced graphene nanosheet and multienzyme functionalized Au@ZnO composites for ultrasensitive electrochemical detection of tumor biomarker. AU - Fang,Xian, AU - Liu,Junfeng, AU - Wang,Jing, AU - Zhao,Hong, AU - Ren,Hongxuan, AU - Li,Zengxi, Y1 - 2017/05/31/ PY - 2017/03/02/received PY - 2017/05/28/revised PY - 2017/05/30/accepted PY - 2017/6/11/pubmed PY - 2018/5/1/medline PY - 2017/6/11/entrez KW - Au nanoparticle/ZnO nanorods hybridized reduced graphene KW - Au@ZnO composite KW - CxN3 KW - Sandwich-type electrochemical immunosensor KW - Signal amplification SP - 218 EP - 225 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 97 N2 - Herein, a dual signal amplification strategy was employed in fabricating ultrasensitive electrochemical immunosensor for alpha fetoprotein (AFP) detection, which was realized by utilizing of ZnO nanorods/Au nanopaticles hybridized reduced graphene nanosheet (Au/ZnO/RGO) and horseradish-peroxidase (HRP) bioconjugated detection antibody (Ab2) functionalized Au@ZnO (Ab2/HRP-Au@ZnO). During the fabrication of the immunosensor, a new kind of multiple-head surfactants CxN3 with different alkyl chain length played important roles such as acting as the surfactants of Au/ZnO/RGO and the reductant agents of Au@ZnO composite. Due to the good adsorption property and large surface area of Au/ZnO/RGO, plenty of the capture antibodies (Ab1) were immobilized on the electrode surface, and trace AFP was sensitively monitored. Furthermore, Ab2/HRP-Au@ZnO exhibited high affinity interaction with AFP through "sandwich" immunoreactions, along with the peroxidase-like catalytic activity of Au@ZnO, leading to a further enhancement in the sensitivity of the proposed immunosensor. The successful synthesis of the nanomaterials was characterized through a serious of techniques including Raman, XRD, FT-IR, SEM and UV-vis. Under the optimal conditions, two linear ranges of 0.02-10,000 and 10,000-100,000pgmL-1 AFP with a lower detection limit of 0.01pg mL-1 (S/N=3) was obtained. Especially, the proposed AFP immunosensor can be applied to detect human serum samples with satisfactory results, indicating a potential application in clinical monitoring of tumor biomarkers. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/28600990/Dual_signal_amplification_strategy_of_Au_nanopaticles/ZnO_nanorods_hybridized_reduced_graphene_nanosheet_and_multienzyme_functionalized_Au@ZnO_composites_for_ultrasensitive_electrochemical_detection_of_tumor_biomarker_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(17)30373-1 DB - PRIME DP - Unbound Medicine ER -