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Rad52 Inverse Strand Exchange Drives RNA-Templated DNA Double-Strand Break Repair.
Mol Cell. 2017 Jul 06; 67(1):19-29.e3.MC

Abstract

RNA can serve as a template for DNA double-strand break repair in yeast cells, and Rad52, a member of the homologous recombination pathway, emerged as an important player in this process. However, the exact mechanism of how Rad52 contributes to RNA-dependent DSB repair remained unknown. Here, we report an unanticipated activity of yeast and human Rad52: inverse strand exchange, in which Rad52 forms a complex with dsDNA and promotes strand exchange with homologous ssRNA or ssDNA. We show that in eukaryotes, inverse strand exchange between homologous dsDNA and RNA is a distinctive activity of Rad52; neither Rad51 recombinase nor the yeast Rad52 paralog Rad59 has this activity. In accord with our in vitro results, our experiments in budding yeast provide evidence that Rad52 inverse strand exchange plays an important role in RNA-templated DSB repair in vivo.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA.School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA.Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA.School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA. Electronic address: storici@gatech.edu.Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA. Electronic address: amazin@drexelmed.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28602639

Citation

Mazina, Olga M., et al. "Rad52 Inverse Strand Exchange Drives RNA-Templated DNA Double-Strand Break Repair." Molecular Cell, vol. 67, no. 1, 2017, pp. 19-29.e3.
Mazina OM, Keskin H, Hanamshet K, et al. Rad52 Inverse Strand Exchange Drives RNA-Templated DNA Double-Strand Break Repair. Mol Cell. 2017;67(1):19-29.e3.
Mazina, O. M., Keskin, H., Hanamshet, K., Storici, F., & Mazin, A. V. (2017). Rad52 Inverse Strand Exchange Drives RNA-Templated DNA Double-Strand Break Repair. Molecular Cell, 67(1), 19-e3. https://doi.org/10.1016/j.molcel.2017.05.019
Mazina OM, et al. Rad52 Inverse Strand Exchange Drives RNA-Templated DNA Double-Strand Break Repair. Mol Cell. 2017 Jul 6;67(1):19-29.e3. PubMed PMID: 28602639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rad52 Inverse Strand Exchange Drives RNA-Templated DNA Double-Strand Break Repair. AU - Mazina,Olga M, AU - Keskin,Havva, AU - Hanamshet,Kritika, AU - Storici,Francesca, AU - Mazin,Alexander V, Y1 - 2017/06/08/ PY - 2017/01/03/received PY - 2017/05/09/revised PY - 2017/05/19/accepted PY - 2017/6/13/pubmed PY - 2017/9/28/medline PY - 2017/6/13/entrez KW - DNA end resection KW - DNA strand exchange KW - DNA-RNA pairing KW - RNA-dependent DNA repair KW - Rad51 KW - Rad52 N-terminal domain KW - Rad59 KW - Sae2 KW - double-strand DNA repair KW - homologous recombination SP - 19 EP - 29.e3 JF - Molecular cell JO - Mol Cell VL - 67 IS - 1 N2 - RNA can serve as a template for DNA double-strand break repair in yeast cells, and Rad52, a member of the homologous recombination pathway, emerged as an important player in this process. However, the exact mechanism of how Rad52 contributes to RNA-dependent DSB repair remained unknown. Here, we report an unanticipated activity of yeast and human Rad52: inverse strand exchange, in which Rad52 forms a complex with dsDNA and promotes strand exchange with homologous ssRNA or ssDNA. We show that in eukaryotes, inverse strand exchange between homologous dsDNA and RNA is a distinctive activity of Rad52; neither Rad51 recombinase nor the yeast Rad52 paralog Rad59 has this activity. In accord with our in vitro results, our experiments in budding yeast provide evidence that Rad52 inverse strand exchange plays an important role in RNA-templated DSB repair in vivo. SN - 1097-4164 UR - https://www.unboundmedicine.com/medline/citation/28602639/Rad52_Inverse_Strand_Exchange_Drives_RNA_Templated_DNA_Double_Strand_Break_Repair_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1097-2765(17)30359-3 DB - PRIME DP - Unbound Medicine ER -