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[Effects of vasoactive intestinal peptide on airway inflammation and Th17/Treg balance in asthmatic mice].
Zhongguo Dang Dai Er Ke Za Zhi. 2017 Jun; 19(6):699-704.ZD

Abstract

OBJECTIVE

To investigate the effects of vasoactive intestinal peptide (VIP) on the airway inflammation and its regulatory effect on Th17/Treg imbalance in asthmatic mice.

METHODS

A total of 30 BALB/c mice were equally and randomly divided into three groups: control, asthma, and VIP. An acute asthmatic mouse model was established by sensitization and challenge with ovalbumin (OVA). The control group received normal saline instead of OVA. Before the challenge with OVA, the VIP group was administered VIP (20 μg/mL) by aerosol inhalation for 30 minutes. The bronchoalveolar lavage fluid (BALF) and the lung tissue were collected from mice. The pathological changes in the lung tissue were observed by hematoxylin and eosin staining. The levels of Th17/Treg-related cytokines in BALF were measured by enzyme-linked immunosorbent assay. The expression of retinoid-related orphan receptor gamma t (RORγt) and forkhead box P3 (Foxp3) were measured by real-time fluorescence quantitative PCR and immunohistochemistry.

RESULTS

The histopathological results showed that the VIP group had milder symptoms of airway inflammation than the asthma group. The level of IL-17 in BALF in the asthma group was significantly higher than that in the control group and the VIP group (P<0.01), but the level of IL-17 in the control group was significantly lower than that in the VIP group (P<0.01). The level of IL-10 in BALF in the asthma group was significantly lower than that in the control group and the VIP group (P<0.01, but the level of IL-10 in the VIP group was significantly higher than that in the control group (P<0.01). The asthma group showed significantly higher expression levels of RORγt mRNA and protein in the lung tissue and significantly lower expression levels of Foxp3 mRNA and protein than the control group (P<0.01). The VIP group had significantly lower expression levels of RORγt mRNA and protein in the lung tissue and significantly higher expression levels of Foxp3 mRNA and protein than the asthma group (P<0.05).

CONCLUSIONS

The Th17/Treg imbalance may be closely related to the airway inflammation in asthmatic mice. VIP can improve airway inflammation by regulating the Th17/Treg imbalance in asthmatic mice.

Authors+Show Affiliations

Department of Pulmonology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. luoyunchun501@163.com.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

28606240

Citation

Ke, Li-Qin, et al. "[Effects of Vasoactive Intestinal Peptide On Airway Inflammation and Th17/Treg Balance in Asthmatic Mice]." Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, vol. 19, no. 6, 2017, pp. 699-704.
Ke LQ, Wang FM, Luo YC. [Effects of vasoactive intestinal peptide on airway inflammation and Th17/Treg balance in asthmatic mice]. Zhongguo Dang Dai Er Ke Za Zhi. 2017;19(6):699-704.
Ke, L. Q., Wang, F. M., & Luo, Y. C. (2017). [Effects of vasoactive intestinal peptide on airway inflammation and Th17/Treg balance in asthmatic mice]. Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, 19(6), 699-704.
Ke LQ, Wang FM, Luo YC. [Effects of Vasoactive Intestinal Peptide On Airway Inflammation and Th17/Treg Balance in Asthmatic Mice]. Zhongguo Dang Dai Er Ke Za Zhi. 2017;19(6):699-704. PubMed PMID: 28606240.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of vasoactive intestinal peptide on airway inflammation and Th17/Treg balance in asthmatic mice]. AU - Ke,Li-Qin, AU - Wang,Feng-Mei, AU - Luo,Yun-Chun, PY - 2017/6/14/entrez PY - 2017/6/14/pubmed PY - 2017/9/1/medline SP - 699 EP - 704 JF - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics JO - Zhongguo Dang Dai Er Ke Za Zhi VL - 19 IS - 6 N2 - OBJECTIVE: To investigate the effects of vasoactive intestinal peptide (VIP) on the airway inflammation and its regulatory effect on Th17/Treg imbalance in asthmatic mice. METHODS: A total of 30 BALB/c mice were equally and randomly divided into three groups: control, asthma, and VIP. An acute asthmatic mouse model was established by sensitization and challenge with ovalbumin (OVA). The control group received normal saline instead of OVA. Before the challenge with OVA, the VIP group was administered VIP (20 μg/mL) by aerosol inhalation for 30 minutes. The bronchoalveolar lavage fluid (BALF) and the lung tissue were collected from mice. The pathological changes in the lung tissue were observed by hematoxylin and eosin staining. The levels of Th17/Treg-related cytokines in BALF were measured by enzyme-linked immunosorbent assay. The expression of retinoid-related orphan receptor gamma t (RORγt) and forkhead box P3 (Foxp3) were measured by real-time fluorescence quantitative PCR and immunohistochemistry. RESULTS: The histopathological results showed that the VIP group had milder symptoms of airway inflammation than the asthma group. The level of IL-17 in BALF in the asthma group was significantly higher than that in the control group and the VIP group (P<0.01), but the level of IL-17 in the control group was significantly lower than that in the VIP group (P<0.01). The level of IL-10 in BALF in the asthma group was significantly lower than that in the control group and the VIP group (P<0.01, but the level of IL-10 in the VIP group was significantly higher than that in the control group (P<0.01). The asthma group showed significantly higher expression levels of RORγt mRNA and protein in the lung tissue and significantly lower expression levels of Foxp3 mRNA and protein than the control group (P<0.01). The VIP group had significantly lower expression levels of RORγt mRNA and protein in the lung tissue and significantly higher expression levels of Foxp3 mRNA and protein than the asthma group (P<0.05). CONCLUSIONS: The Th17/Treg imbalance may be closely related to the airway inflammation in asthmatic mice. VIP can improve airway inflammation by regulating the Th17/Treg imbalance in asthmatic mice. SN - 1008-8830 UR - https://www.unboundmedicine.com/medline/citation/28606240/[Effects_of_vasoactive_intestinal_peptide_on_airway_inflammation_and_Th17/Treg_balance_in_asthmatic_mice]_ DB - PRIME DP - Unbound Medicine ER -