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Dioscin suppresses TGF-β1-induced epithelial-mesenchymal transition and suppresses A549 lung cancer migration and invasion.
Bioorg Med Chem Lett. 2017 08 01; 27(15):3342-3348.BM

Abstract

Epithelial-to-mesenchymal transition (EMT), an important cellular process, occurs during cancer development and progression, has a crucial role in metastasis by enhancing the motility of tumor cells. Dioscin is a polyphenolic component isolated from Phyllanthus amarus, which exhibits a wide range of pharmacological and physiological activities, such as anti-tumor, anti-inflammatory, anti-obesity, anti-fungal, and anti-viral activities. However, the possible role of dioscin in the EMT is unclear. We investigated the suppressive effect of dioscin on the EMT. Transforming growth factor-beta 1 (TGF-β1) is known to induce EMT in a number of cancer cell types and promote lung adenocarcinoma migration and invasion. To verify the inhibitory role of dioscin in lung cancer migration and invasion, we investigated the use of dioscin as inhibitors of TGF-β1-induced EMT in A549 lung cancer cells in vitro. Here, we found that dioscin prominently increased expression of the epithelial marker E-cadherin and expression of the mesenchymal marker N-cadherin and Snail during the TGF-β1-induced EMT. In addition, dioscin inhibited the TGF-β1-induced increase in cell migration and invasion of A549 lung cancer cells. Also, dioscin remarkably inhibited TGF-β1-regulated activation of MMP-2/9, Smad2, and p38. Taken together, our findings provide new evidence that dioscin suppresses lung cancer migration, and invasion in vitro by inhibiting the TGF-β1-induced EMT.

Authors+Show Affiliations

Laboratory of Molecular Oncology, Cheil General Hospital & Women's Healthcare Center, College of Medicine, Dankook University, Seoul, South Korea.Laboratory of Molecular Oncology, Cheil General Hospital & Women's Healthcare Center, College of Medicine, Dankook University, Seoul, South Korea.Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do, South Korea.Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea; Department of Pharmacology, University of Ulsan College of Medicine, Seoul, South Korea.Department of Obstetrics and Gynecology, Cheil General Hospital & Women's Healthcare Center, College of Medicine, Dankook University, Seoul, South Korea.Department of Obstetrics and Gynecology, Cheil General Hospital & Women's Healthcare Center, College of Medicine, Dankook University, Seoul, South Korea.Department of Obstetrics and Gynecology, Cheil General Hospital & Women's Healthcare Center, College of Medicine, Dankook University, Seoul, South Korea.Laboratory of Molecular Oncology, Cheil General Hospital & Women's Healthcare Center, College of Medicine, Dankook University, Seoul, South Korea. Electronic address: drug9054@naver.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28610976

Citation

Lim, Won-Chul, et al. "Dioscin Suppresses TGF-β1-induced Epithelial-mesenchymal Transition and Suppresses A549 Lung Cancer Migration and Invasion." Bioorganic & Medicinal Chemistry Letters, vol. 27, no. 15, 2017, pp. 3342-3348.
Lim WC, Kim H, Kim YJ, et al. Dioscin suppresses TGF-β1-induced epithelial-mesenchymal transition and suppresses A549 lung cancer migration and invasion. Bioorg Med Chem Lett. 2017;27(15):3342-3348.
Lim, W. C., Kim, H., Kim, Y. J., Choi, K. C., Lee, I. H., Lee, K. H., Kim, M. K., & Ko, H. (2017). Dioscin suppresses TGF-β1-induced epithelial-mesenchymal transition and suppresses A549 lung cancer migration and invasion. Bioorganic & Medicinal Chemistry Letters, 27(15), 3342-3348. https://doi.org/10.1016/j.bmcl.2017.06.014
Lim WC, et al. Dioscin Suppresses TGF-β1-induced Epithelial-mesenchymal Transition and Suppresses A549 Lung Cancer Migration and Invasion. Bioorg Med Chem Lett. 2017 08 1;27(15):3342-3348. PubMed PMID: 28610976.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dioscin suppresses TGF-β1-induced epithelial-mesenchymal transition and suppresses A549 lung cancer migration and invasion. AU - Lim,Won-Chul, AU - Kim,Hyunhee, AU - Kim,Young-Joo, AU - Choi,Kyung-Chul, AU - Lee,In Ho, AU - Lee,Ki Heon, AU - Kim,Mi Kyung, AU - Ko,Hyeonseok, Y1 - 2017/06/03/ PY - 2017/05/16/received PY - 2017/05/31/revised PY - 2017/06/02/accepted PY - 2017/6/15/pubmed PY - 2017/8/16/medline PY - 2017/6/15/entrez KW - Dioscin KW - Epithelial–mesenchymal transition (EMT) KW - Invasion KW - Lung cancer KW - Migration KW - Transforming growth factor-beta 1 (TGF-β1) SP - 3342 EP - 3348 JF - Bioorganic & medicinal chemistry letters JO - Bioorg Med Chem Lett VL - 27 IS - 15 N2 - Epithelial-to-mesenchymal transition (EMT), an important cellular process, occurs during cancer development and progression, has a crucial role in metastasis by enhancing the motility of tumor cells. Dioscin is a polyphenolic component isolated from Phyllanthus amarus, which exhibits a wide range of pharmacological and physiological activities, such as anti-tumor, anti-inflammatory, anti-obesity, anti-fungal, and anti-viral activities. However, the possible role of dioscin in the EMT is unclear. We investigated the suppressive effect of dioscin on the EMT. Transforming growth factor-beta 1 (TGF-β1) is known to induce EMT in a number of cancer cell types and promote lung adenocarcinoma migration and invasion. To verify the inhibitory role of dioscin in lung cancer migration and invasion, we investigated the use of dioscin as inhibitors of TGF-β1-induced EMT in A549 lung cancer cells in vitro. Here, we found that dioscin prominently increased expression of the epithelial marker E-cadherin and expression of the mesenchymal marker N-cadherin and Snail during the TGF-β1-induced EMT. In addition, dioscin inhibited the TGF-β1-induced increase in cell migration and invasion of A549 lung cancer cells. Also, dioscin remarkably inhibited TGF-β1-regulated activation of MMP-2/9, Smad2, and p38. Taken together, our findings provide new evidence that dioscin suppresses lung cancer migration, and invasion in vitro by inhibiting the TGF-β1-induced EMT. SN - 1464-3405 UR - https://www.unboundmedicine.com/medline/citation/28610976/Dioscin_suppresses_TGF_β1_induced_epithelial_mesenchymal_transition_and_suppresses_A549_lung_cancer_migration_and_invasion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-894X(17)30616-9 DB - PRIME DP - Unbound Medicine ER -