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Exploiting antidiabetic activity of silver nanoparticles synthesized using Punica granatum leaves and anticancer potential against human liver cancer cells (HepG2).
Artif Cells Nanomed Biotechnol. 2018 Feb; 46(1):211-222.AC

Abstract

This study first time reports the novel synthesis of silver nanoparticles (AgNPs) using a Punica granatum leaf extract (PGE). The synthesized AgNPs were characterized by various analytical techniques including UV-Vis, Fourier transform infrared (FTIR), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy and energy-dispersive spectra (FESEM-EDS) and high-resolution transmission electron microscopy (HRTEM). FTIR analysis revealed that the involvement of biological macromolecules of P. granatum leaf extract were distributed and involved in the synthesis and stabilization of AgNPs. A surface-sensitive technique of XPS was used to analyse the composition and oxidation state of synthesized AgNPs. The analytical results confirmed that the AgNPs were crystalline in nature with spherical shape. The zeta potential study revealed that the surface charge of synthesized AgNPs was highly negative (-26.6 mV) and particle size distribution was ranging from ∼35 to 60 nm and the average particle size was about 48 nm determined by dynamic light scattering (DLS). The PGE-AgNPs antidiabetic potential exhibited effective inhibition against α-amylase and α-glucosidase (IC50; 65.2 and 53.8 μg/mL, respectively). The PGE-AgNPs showed a dose-dependent response against human liver cancer cells (HepG2) (IC50; 70 μg/mL) indicating its greater efficacy in killing cancer cells. They also possessed in vitro free radical-scavenging activity in terms of ABTS (IC50; 52.2 μg/mL) and DPPH (IC50; 67.1 μg/mL) antioxidant activity. PGE-AgNPs displayed strong antibacterial activity and potent synergy with standard antibiotics against pathogenic bacteria. Thus, synthesized PGE-AgNPs show potential biomedical and industrial applications.

Authors+Show Affiliations

a Research Institute of Biotechnology and Medical Converged Science , Dongguk University-Seoul , Goyang-si , Republic of Korea.b Department of Food Science and Biotechnology , Dongguk University-Seoul , Goyang-si , Republic of Korea.c Department of Environmental Engineering , Daegu University , South Korea.d Department of Agriculture, Food and Environment , University of Pisa , Pisa , Italy.e Department of Environmental Science and Engineering , Ewha Womans University , Seoul , South Korea.b Department of Food Science and Biotechnology , Dongguk University-Seoul , Goyang-si , Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28612655

Citation

Saratale, Rijuta G., et al. "Exploiting Antidiabetic Activity of Silver Nanoparticles Synthesized Using Punica Granatum Leaves and Anticancer Potential Against Human Liver Cancer Cells (HepG2)." Artificial Cells, Nanomedicine, and Biotechnology, vol. 46, no. 1, 2018, pp. 211-222.
Saratale RG, Shin HS, Kumar G, et al. Exploiting antidiabetic activity of silver nanoparticles synthesized using Punica granatum leaves and anticancer potential against human liver cancer cells (HepG2). Artif Cells Nanomed Biotechnol. 2018;46(1):211-222.
Saratale, R. G., Shin, H. S., Kumar, G., Benelli, G., Kim, D. S., & Saratale, G. D. (2018). Exploiting antidiabetic activity of silver nanoparticles synthesized using Punica granatum leaves and anticancer potential against human liver cancer cells (HepG2). Artificial Cells, Nanomedicine, and Biotechnology, 46(1), 211-222. https://doi.org/10.1080/21691401.2017.1337031
Saratale RG, et al. Exploiting Antidiabetic Activity of Silver Nanoparticles Synthesized Using Punica Granatum Leaves and Anticancer Potential Against Human Liver Cancer Cells (HepG2). Artif Cells Nanomed Biotechnol. 2018;46(1):211-222. PubMed PMID: 28612655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exploiting antidiabetic activity of silver nanoparticles synthesized using Punica granatum leaves and anticancer potential against human liver cancer cells (HepG2). AU - Saratale,Rijuta G, AU - Shin,Han Seung, AU - Kumar,Gopalakrishnan, AU - Benelli,Giovanni, AU - Kim,Dong-Su, AU - Saratale,Ganesh D, Y1 - 2017/06/14/ PY - 2017/6/15/pubmed PY - 2018/8/2/medline PY - 2017/6/15/entrez KW - Punica granatum leaf extract KW - anticancer activity KW - liver cancer HepG2 cell line; in vitro antioxidant activity KW - α-Glucosidase SP - 211 EP - 222 JF - Artificial cells, nanomedicine, and biotechnology JO - Artif Cells Nanomed Biotechnol VL - 46 IS - 1 N2 - This study first time reports the novel synthesis of silver nanoparticles (AgNPs) using a Punica granatum leaf extract (PGE). The synthesized AgNPs were characterized by various analytical techniques including UV-Vis, Fourier transform infrared (FTIR), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy and energy-dispersive spectra (FESEM-EDS) and high-resolution transmission electron microscopy (HRTEM). FTIR analysis revealed that the involvement of biological macromolecules of P. granatum leaf extract were distributed and involved in the synthesis and stabilization of AgNPs. A surface-sensitive technique of XPS was used to analyse the composition and oxidation state of synthesized AgNPs. The analytical results confirmed that the AgNPs were crystalline in nature with spherical shape. The zeta potential study revealed that the surface charge of synthesized AgNPs was highly negative (-26.6 mV) and particle size distribution was ranging from ∼35 to 60 nm and the average particle size was about 48 nm determined by dynamic light scattering (DLS). The PGE-AgNPs antidiabetic potential exhibited effective inhibition against α-amylase and α-glucosidase (IC50; 65.2 and 53.8 μg/mL, respectively). The PGE-AgNPs showed a dose-dependent response against human liver cancer cells (HepG2) (IC50; 70 μg/mL) indicating its greater efficacy in killing cancer cells. They also possessed in vitro free radical-scavenging activity in terms of ABTS (IC50; 52.2 μg/mL) and DPPH (IC50; 67.1 μg/mL) antioxidant activity. PGE-AgNPs displayed strong antibacterial activity and potent synergy with standard antibiotics against pathogenic bacteria. Thus, synthesized PGE-AgNPs show potential biomedical and industrial applications. SN - 2169-141X UR - https://www.unboundmedicine.com/medline/citation/28612655/Exploiting_antidiabetic_activity_of_silver_nanoparticles_synthesized_using_Punica_granatum_leaves_and_anticancer_potential_against_human_liver_cancer_cells__HepG2__ L2 - https://www.tandfonline.com/doi/full/10.1080/21691401.2017.1337031 DB - PRIME DP - Unbound Medicine ER -