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Silencing of a lipase maturation factor 2-like gene by wheat-mediated RNAi reduces the survivability and reproductive capacity of the grain aphid, Sitobion avenae.
Arch Insect Biochem Physiol. 2017 Jul; 95(3)AI

Abstract

Lipase maturation factor (LMF) family proteins are required for the maturation and transport of active lipoprotein lipases. However, the specific roles of LMF2 remain unknown. In this study, a grain aphid lmf2-like gene fragment was cloned and was highly similar in sequence to a homologous gene in the pea aphid, Acyrthosiphon pisum. An RNAi vector was constructed with this fragment and used for wheat transformation. The expression of the lmf2-like gene in aphid, as well as the growth and reproduction of the aphids, was analyzed after feeding on the transgenic wheat. There were no significant differences in the expression of the lmf2-like gene over development. The expression of the lmf2-like gene was significantly reduced by 27.6% on the fifth day, and 57.6% on the 10th day after feeding. The total number of aphids produced on the transgenic plants was less than the number produced on control plants, and the difference became significant or after 2 weeks. The molting numbers were also reduced in the aphids reared on the transgenic plants. Our findings indicate that lmf2-like genes may have potential as a target gene for the control of grain aphids and show that feeding aphids with wheat expressing lmf2-like RNAi resulted in significant reductions in survival and reproduction.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Xu L
State Key Laboratory for Agrobiotechnology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China. Beijing Key Laboratory of Crop Genetic Improvement, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
Hou Q
State Key Laboratory for Agrobiotechnology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China. Beijing Key Laboratory of Crop Genetic Improvement, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
Zhao Y
State Key Laboratory for Agrobiotechnology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China. Beijing Key Laboratory of Crop Genetic Improvement, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
Lu L
State Key Laboratory for Agrobiotechnology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China. Beijing Key Laboratory of Crop Genetic Improvement, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
Li B
State Key Laboratory for Agrobiotechnology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China. Beijing Key Laboratory of Crop Genetic Improvement, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
Ni Z
State Key Laboratory for Agrobiotechnology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China. Beijing Key Laboratory of Crop Genetic Improvement, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
Liang R
State Key Laboratory for Agrobiotechnology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China. Beijing Key Laboratory of Crop Genetic Improvement, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.

MeSH

Amino Acid SequenceAnimalsAphidsInsect ControlInsect ProteinsMoltingPlants, Genetically ModifiedRNA InterferenceReproductionSequence Analysis, DNATriticum

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

28618004

Citation

Xu, Lanjie, et al. "Silencing of a Lipase Maturation Factor 2-like Gene By Wheat-mediated RNAi Reduces the Survivability and Reproductive Capacity of the Grain Aphid, Sitobion Avenae." Archives of Insect Biochemistry and Physiology, vol. 95, no. 3, 2017.
Xu L, Hou Q, Zhao Y, et al. Silencing of a lipase maturation factor 2-like gene by wheat-mediated RNAi reduces the survivability and reproductive capacity of the grain aphid, Sitobion avenae. Arch Insect Biochem Physiol. 2017;95(3).
Xu, L., Hou, Q., Zhao, Y., Lu, L., Li, B., Ni, Z., & Liang, R. (2017). Silencing of a lipase maturation factor 2-like gene by wheat-mediated RNAi reduces the survivability and reproductive capacity of the grain aphid, Sitobion avenae. Archives of Insect Biochemistry and Physiology, 95(3). https://doi.org/10.1002/arch.21392
Xu L, et al. Silencing of a Lipase Maturation Factor 2-like Gene By Wheat-mediated RNAi Reduces the Survivability and Reproductive Capacity of the Grain Aphid, Sitobion Avenae. Arch Insect Biochem Physiol. 2017;95(3) PubMed PMID: 28618004.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silencing of a lipase maturation factor 2-like gene by wheat-mediated RNAi reduces the survivability and reproductive capacity of the grain aphid, Sitobion avenae. AU - Xu,Lanjie, AU - Hou,Qiling, AU - Zhao,Yanjie, AU - Lu,Lihua, AU - Li,Baoyun, AU - Ni,Zhongfu, AU - Liang,Rongqi, Y1 - 2017/06/15/ PY - 2017/6/16/pubmed PY - 2017/10/4/medline PY - 2017/6/16/entrez KW - Common wheat KW - RNAi KW - grain aphid KW - lipase maturation factor (LMF) JF - Archives of insect biochemistry and physiology JO - Arch Insect Biochem Physiol VL - 95 IS - 3 N2 - Lipase maturation factor (LMF) family proteins are required for the maturation and transport of active lipoprotein lipases. However, the specific roles of LMF2 remain unknown. In this study, a grain aphid lmf2-like gene fragment was cloned and was highly similar in sequence to a homologous gene in the pea aphid, Acyrthosiphon pisum. An RNAi vector was constructed with this fragment and used for wheat transformation. The expression of the lmf2-like gene in aphid, as well as the growth and reproduction of the aphids, was analyzed after feeding on the transgenic wheat. There were no significant differences in the expression of the lmf2-like gene over development. The expression of the lmf2-like gene was significantly reduced by 27.6% on the fifth day, and 57.6% on the 10th day after feeding. The total number of aphids produced on the transgenic plants was less than the number produced on control plants, and the difference became significant or after 2 weeks. The molting numbers were also reduced in the aphids reared on the transgenic plants. Our findings indicate that lmf2-like genes may have potential as a target gene for the control of grain aphids and show that feeding aphids with wheat expressing lmf2-like RNAi resulted in significant reductions in survival and reproduction. SN - 1520-6327 UR - https://www.unboundmedicine.com/medline/citation/28618004/Silencing_of_a_lipase_maturation_factor_2_like_gene_by_wheat_mediated_RNAi_reduces_the_survivability_and_reproductive_capacity_of_the_grain_aphid_Sitobion_avenae_ L2 - https://doi.org/10.1002/arch.21392 DB - PRIME DP - Unbound Medicine ER -