Tags

Type your tag names separated by a space and hit enter

Modulatory effects of Caralluma fimbriata extract against high-fat diet induced abnormalities in carbohydrate metabolism in Wistar rats.
Biomed Pharmacother. 2017 Aug; 92:1062-1072.BP

Abstract

The present study was aimed to evaluate the modulatory effects of hydroalcoholic extract of Caralluma fimbriata (CFE) by assaying the activities of key enzymes of carbohydrate metabolism and changes in glycogen content (liver and muscle) in high-fat (HF) diet-induced diabetic rats. In vitro glucose uptake studies were carried out in both psoas muscle and adipose tissue. The inhibitory effect of the extract on α-amylase was determined in in vitro studies. Male Wistar rats of body weight around 180g were divided into five groups (n=8), two of these groups were fed with standard pellet diet and the other three groups were fed with HF- (60%) diet. CFE (200mg/kg body weight/day) was administered through oral route to each group of standard pellet diet rats and HF-fed rats and Metformin (Met) (20mg/kg body weight/day) was administered through oral route to HFD+Met group for 90 days. At the end of the experimental period, biochemical parameters related to glycogen content in liver and muscle, and intestinal disaccharidases like maltase, sucrase and lactase were assayed. Alterations in the activities of enzymes of glucose metabolism (hexokinase, phosphorfructoki nase, pyruvate kinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, and glucose-6-phosphate dehydrogenase), intestinal disaccharidases and glycogen content as observed in the high fat diet-fed rats were prevented with CFE/Met administration. From this study, we observed that CFE/Met could significantly restore the levels of glycogen in liver and muscle and key enzymes of carbohydrate metabolism to near normal in groups-HFD+CFE and HFD+Met. The skeletal muscle of HF-diet fed rats showed degenerative changes of muscle myofibers with fat deposition. These changes were attenuated in the HFD group treated with CFE/Met and retained their normal structure appearance. It can be concluded from these results that CFE might be of value in reducing the alterations related to carbohydrate metabolism under high calorie diet consumption.

Authors+Show Affiliations

Dept. of Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, 515003, India.Dept. of Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, 515003, India.Dept. of Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, 515003, India.Center for Biofluid and Biomimic Research, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.Green Chem Herbal Extract and Formulations, Bangalore, 560071, India.Dept. of Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, 515003, India. Electronic address: skumari1@yahoo.co.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28618651

Citation

Gujjala, Sudhakara, et al. "Modulatory Effects of Caralluma Fimbriata Extract Against High-fat Diet Induced Abnormalities in Carbohydrate Metabolism in Wistar Rats." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 92, 2017, pp. 1062-1072.
Gujjala S, Putakala M, Nukala S, et al. Modulatory effects of Caralluma fimbriata extract against high-fat diet induced abnormalities in carbohydrate metabolism in Wistar rats. Biomed Pharmacother. 2017;92:1062-1072.
Gujjala, S., Putakala, M., Nukala, S., Bangeppagari, M., Rajendran, R., & Desireddy, S. (2017). Modulatory effects of Caralluma fimbriata extract against high-fat diet induced abnormalities in carbohydrate metabolism in Wistar rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 92, 1062-1072. https://doi.org/10.1016/j.biopha.2017.06.016
Gujjala S, et al. Modulatory Effects of Caralluma Fimbriata Extract Against High-fat Diet Induced Abnormalities in Carbohydrate Metabolism in Wistar Rats. Biomed Pharmacother. 2017;92:1062-1072. PubMed PMID: 28618651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulatory effects of Caralluma fimbriata extract against high-fat diet induced abnormalities in carbohydrate metabolism in Wistar rats. AU - Gujjala,Sudhakara, AU - Putakala,Mallaiah, AU - Nukala,Srinivasulu, AU - Bangeppagari,Manjunatha, AU - Rajendran,Ramaswamy, AU - Desireddy,Saralakumari, Y1 - 2017/06/10/ PY - 2017/03/21/received PY - 2017/05/29/revised PY - 2017/06/05/accepted PY - 2017/6/18/pubmed PY - 2018/2/1/medline PY - 2017/6/17/entrez KW - Caralluma fimbriata KW - Hexokinase KW - High-fat diet KW - Intestinal disaccharidases KW - Pyruvate kinase KW - Skeletal muscle SP - 1062 EP - 1072 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed Pharmacother VL - 92 N2 - The present study was aimed to evaluate the modulatory effects of hydroalcoholic extract of Caralluma fimbriata (CFE) by assaying the activities of key enzymes of carbohydrate metabolism and changes in glycogen content (liver and muscle) in high-fat (HF) diet-induced diabetic rats. In vitro glucose uptake studies were carried out in both psoas muscle and adipose tissue. The inhibitory effect of the extract on α-amylase was determined in in vitro studies. Male Wistar rats of body weight around 180g were divided into five groups (n=8), two of these groups were fed with standard pellet diet and the other three groups were fed with HF- (60%) diet. CFE (200mg/kg body weight/day) was administered through oral route to each group of standard pellet diet rats and HF-fed rats and Metformin (Met) (20mg/kg body weight/day) was administered through oral route to HFD+Met group for 90 days. At the end of the experimental period, biochemical parameters related to glycogen content in liver and muscle, and intestinal disaccharidases like maltase, sucrase and lactase were assayed. Alterations in the activities of enzymes of glucose metabolism (hexokinase, phosphorfructoki nase, pyruvate kinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, and glucose-6-phosphate dehydrogenase), intestinal disaccharidases and glycogen content as observed in the high fat diet-fed rats were prevented with CFE/Met administration. From this study, we observed that CFE/Met could significantly restore the levels of glycogen in liver and muscle and key enzymes of carbohydrate metabolism to near normal in groups-HFD+CFE and HFD+Met. The skeletal muscle of HF-diet fed rats showed degenerative changes of muscle myofibers with fat deposition. These changes were attenuated in the HFD group treated with CFE/Met and retained their normal structure appearance. It can be concluded from these results that CFE might be of value in reducing the alterations related to carbohydrate metabolism under high calorie diet consumption. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/28618651/Modulatory_effects_of_Caralluma_fimbriata_extract_against_high_fat_diet_induced_abnormalities_in_carbohydrate_metabolism_in_Wistar_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(17)31325-2 DB - PRIME DP - Unbound Medicine ER -