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Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock.
BMC Vet Res 2017; 13(1):177BV

Abstract

BACKGROUND

Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serovar Hardjo remain important species associated with this reproductive disease in livestock production. Previous studies on Brazilian livestock have reported that L. interrogans serovar Hardjo is the most prevalent leptospiral agent in this country and is related to clinical signs of leptospirosis, which lead to economic losses in production. Here, we described the isolation of three clinical strains (Norma, Lagoa and Bolivia) obtained from leptospirosis outbreaks that occurred in Minas Gerais state in 1994 and 2008.

RESULTS

Serological and molecular typing using housekeeping (secY and 16SrRNA) and rfb locus (ORF22 and ORF36) genes were applied for the identification and comparative analysis of Leptospira spp. Our results identified the three isolates as L. interrogans serogroup Sejroe serovar Hardjo and confirmed the occurrence of this bacterial strain in Brazilian livestock. Genetic analysis using ORF22 and ORF36 grouped the Leptospira into serogroup Sejroe and subtype Hardjoprajitno. Genetic approaches were also applied to compare distinct serovars of L. interrogans strains by verifying the copy numbers of the IS1500 and IS1533 insertion sequences (ISs). The IS1500 copy number varied among the analyzed L. interrogans strains.

CONCLUSION

This study provides evidence that L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjoprajitno causes bovine leptospirosis in Brazilian production. The molecular results suggested that rfb locus (ORF22 and ORF36) could improve epidemiological studies by allowing the identification of Leptospira spp. at the serogroup level. Additionally, the IS1500 and IS1533 IS copy number analysis suggested distinct genomic features among closely related leptospiral strains.

Authors+Show Affiliations

Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. raquelcosate@ymail.com.Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.Laboratório de Biologia Parasitária, Centro de Pesquisas Gonçalo Moniz- Fiocruz, Salvador, BA, Brazil.Embrapa Agroenergia, Brasília, Brazil.Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais, Departamento de Genética - Instituto de Ciências Biol'ogicas de Minas Gerais, Belo Horizonte, Brazil.Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28619055

Citation

Cosate, Maria Raquel V., et al. "Molecular Typing of Leptospira Interrogans Serovar Hardjo Isolates From Leptospirosis Outbreaks in Brazilian Livestock." BMC Veterinary Research, vol. 13, no. 1, 2017, p. 177.
Cosate MRV, Sakamoto T, de Oliveira Mendes TA, et al. Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock. BMC Vet Res. 2017;13(1):177.
Cosate, M. R. V., Sakamoto, T., de Oliveira Mendes, T. A., Moreira, É. C., Regis da Silva, C. G., Brasil, B. S. A. F., ... Haddad, J. P. (2017). Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock. BMC Veterinary Research, 13(1), p. 177. doi:10.1186/s12917-017-1081-9.
Cosate MRV, et al. Molecular Typing of Leptospira Interrogans Serovar Hardjo Isolates From Leptospirosis Outbreaks in Brazilian Livestock. BMC Vet Res. 2017 Jun 15;13(1):177. PubMed PMID: 28619055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock. AU - Cosate,Maria Raquel V, AU - Sakamoto,Tetsu, AU - de Oliveira Mendes,Tiago Antônio, AU - Moreira,Élvio C, AU - Regis da Silva,Carlos G, AU - Brasil,Bruno S A F, AU - Oliveira,Camila S F, AU - de Azevedo,Vasco Ariston, AU - Ortega,José Miguel, AU - Leite,Rômulo C, AU - Haddad,João Paulo, Y1 - 2017/06/15/ PY - 2016/04/04/received PY - 2017/05/30/accepted PY - 2017/6/17/entrez PY - 2017/6/18/pubmed PY - 2017/11/8/medline SP - 177 EP - 177 JF - BMC veterinary research JO - BMC Vet. Res. VL - 13 IS - 1 N2 - BACKGROUND: Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serovar Hardjo remain important species associated with this reproductive disease in livestock production. Previous studies on Brazilian livestock have reported that L. interrogans serovar Hardjo is the most prevalent leptospiral agent in this country and is related to clinical signs of leptospirosis, which lead to economic losses in production. Here, we described the isolation of three clinical strains (Norma, Lagoa and Bolivia) obtained from leptospirosis outbreaks that occurred in Minas Gerais state in 1994 and 2008. RESULTS: Serological and molecular typing using housekeeping (secY and 16SrRNA) and rfb locus (ORF22 and ORF36) genes were applied for the identification and comparative analysis of Leptospira spp. Our results identified the three isolates as L. interrogans serogroup Sejroe serovar Hardjo and confirmed the occurrence of this bacterial strain in Brazilian livestock. Genetic analysis using ORF22 and ORF36 grouped the Leptospira into serogroup Sejroe and subtype Hardjoprajitno. Genetic approaches were also applied to compare distinct serovars of L. interrogans strains by verifying the copy numbers of the IS1500 and IS1533 insertion sequences (ISs). The IS1500 copy number varied among the analyzed L. interrogans strains. CONCLUSION: This study provides evidence that L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjoprajitno causes bovine leptospirosis in Brazilian production. The molecular results suggested that rfb locus (ORF22 and ORF36) could improve epidemiological studies by allowing the identification of Leptospira spp. at the serogroup level. Additionally, the IS1500 and IS1533 IS copy number analysis suggested distinct genomic features among closely related leptospiral strains. SN - 1746-6148 UR - https://www.unboundmedicine.com/medline/citation/28619055/Molecular_typing_of_Leptospira_interrogans_serovar_Hardjo_isolates_from_leptospirosis_outbreaks_in_Brazilian_livestock_ L2 - https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1081-9 DB - PRIME DP - Unbound Medicine ER -