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Anti-inflammatory effect of Chang-An-Shuan on TNBS-induced experimental colitis in rats.
BMC Complement Altern Med. 2017 Jun 15; 17(1):315.BC

Abstract

BACKGROUND

Inflammatory bowel disease (IBD), denominated by Crohn's disease and ulcerative colitis, is often associated with abdominal pain, diarrhea and bloody stool. The standard protocols for treating colitis conditions are not satisfactory; thus, complementary and alternative medicines have been increasingly accepted by IBD sufferers worldwide. In this study, we aimed to elucidate the anti-inflammatory effect of Chang-An-Shuan (CAS), a 6-herb Chinese medicinal formula, on 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats and the underlying mechanisms.

METHODS

Sprague-Dawley rats were administered with rectal gavage of 2.5% TNBS in 50% ethanol for the induction of experimental colitis which is considered as a model for Crohn's disease. Upon the TNBS induction, rats were given CAS at 0.5 g/kg/day or 5 g/kg/day for 10 days. The application of salicylazosulfapyridine (0.5 g/kg/day) was served as a positive reference drug for the colitis condition. The efficacy and mechanistic action of CAS were evaluated by means of histopathological and biochemical approaches such as histological staining, real-time polymerase chain reaction, Western blotting analysis and enzyme-linked immunosorbent assay.

RESULTS

Oral administration of CAS at 5 g/kg/day, but not 0.5 g/kg/day, significantly ameliorated the severity of TNBS-induced colitis as evidenced by the reduced loss of body weight, alleviated diarrhea and decreased bloody stool. While lowering the disease activity index, the administration of CAS lessened mucosal lesions thus mucosal integrity of the colitis rats was notably improved. Further, the CAS treatment also significantly suppressed the mRNA and protein levels of pro-inflammatory cytokines, namely interleukin-1β and tumor necrosis factor-α while enhancing the level of anti-inflammatory cytokine IL-10 in the TNBS-treated rats. Importantly, the ameliorative effect of CAS was related to an inhibition of the nuclear factor-κB (NF-κB) signaling pathway by downregulating the expression levels of NF-κBp-65, p-38 and p-AKT.

CONCLUSIONS

We suggest that CAS is a potential alternative remedial approach for treating IBD conditions, and the anti-inflammatory effect of CAS is associated with the down-regulation of the NF-κB signaling pathway and the balanced production of pro- and anti-inflammatory cytokines.

Authors+Show Affiliations

Department of Gastroenterology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.Department of Gastroenterology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China. liufb163@163.com.First School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.Department of Gastroenterology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.Department of Gastroenterology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28619075

Citation

Mi, Hong, et al. "Anti-inflammatory Effect of Chang-An-Shuan On TNBS-induced Experimental Colitis in Rats." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 315.
Mi H, Liu FB, Li HW, et al. Anti-inflammatory effect of Chang-An-Shuan on TNBS-induced experimental colitis in rats. BMC Complement Altern Med. 2017;17(1):315.
Mi, H., Liu, F. B., Li, H. W., Hou, J. T., & Li, P. W. (2017). Anti-inflammatory effect of Chang-An-Shuan on TNBS-induced experimental colitis in rats. BMC Complementary and Alternative Medicine, 17(1), 315. https://doi.org/10.1186/s12906-017-1794-0
Mi H, et al. Anti-inflammatory Effect of Chang-An-Shuan On TNBS-induced Experimental Colitis in Rats. BMC Complement Altern Med. 2017 Jun 15;17(1):315. PubMed PMID: 28619075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effect of Chang-An-Shuan on TNBS-induced experimental colitis in rats. AU - Mi,Hong, AU - Liu,Feng-Bin, AU - Li,Hai-Wen, AU - Hou,Jiang-Tao, AU - Li,Pei-Wu, Y1 - 2017/06/15/ PY - 2016/05/03/received PY - 2017/05/15/accepted PY - 2017/6/17/entrez PY - 2017/6/18/pubmed PY - 2017/8/22/medline KW - Chang-An-Shuan KW - Interleukin-1β KW - Nuclear factor-κB KW - Tumor necrosis factor-α KW - Ulcerative colitis SP - 315 EP - 315 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Inflammatory bowel disease (IBD), denominated by Crohn's disease and ulcerative colitis, is often associated with abdominal pain, diarrhea and bloody stool. The standard protocols for treating colitis conditions are not satisfactory; thus, complementary and alternative medicines have been increasingly accepted by IBD sufferers worldwide. In this study, we aimed to elucidate the anti-inflammatory effect of Chang-An-Shuan (CAS), a 6-herb Chinese medicinal formula, on 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats and the underlying mechanisms. METHODS: Sprague-Dawley rats were administered with rectal gavage of 2.5% TNBS in 50% ethanol for the induction of experimental colitis which is considered as a model for Crohn's disease. Upon the TNBS induction, rats were given CAS at 0.5 g/kg/day or 5 g/kg/day for 10 days. The application of salicylazosulfapyridine (0.5 g/kg/day) was served as a positive reference drug for the colitis condition. The efficacy and mechanistic action of CAS were evaluated by means of histopathological and biochemical approaches such as histological staining, real-time polymerase chain reaction, Western blotting analysis and enzyme-linked immunosorbent assay. RESULTS: Oral administration of CAS at 5 g/kg/day, but not 0.5 g/kg/day, significantly ameliorated the severity of TNBS-induced colitis as evidenced by the reduced loss of body weight, alleviated diarrhea and decreased bloody stool. While lowering the disease activity index, the administration of CAS lessened mucosal lesions thus mucosal integrity of the colitis rats was notably improved. Further, the CAS treatment also significantly suppressed the mRNA and protein levels of pro-inflammatory cytokines, namely interleukin-1β and tumor necrosis factor-α while enhancing the level of anti-inflammatory cytokine IL-10 in the TNBS-treated rats. Importantly, the ameliorative effect of CAS was related to an inhibition of the nuclear factor-κB (NF-κB) signaling pathway by downregulating the expression levels of NF-κBp-65, p-38 and p-AKT. CONCLUSIONS: We suggest that CAS is a potential alternative remedial approach for treating IBD conditions, and the anti-inflammatory effect of CAS is associated with the down-regulation of the NF-κB signaling pathway and the balanced production of pro- and anti-inflammatory cytokines. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28619075/Anti_inflammatory_effect_of_Chang_An_Shuan_on_TNBS_induced_experimental_colitis_in_rats_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1794-0 DB - PRIME DP - Unbound Medicine ER -