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Single gene reassortment of highly pathogenic avian influenza A H5N1 in the low pathogenic H9N2 backbone and its impact on pathogenicity and infectivity of novel reassortant viruses.
Arch Virol. 2017 Oct; 162(10):2959-2969.AV

Abstract

Avian influenza A H5N1 and H9N2 viruses have been extensively circulating in various avian species and frequently infect mammals, including humans. The synchronous circulation of both viruses in Egypt provides an opportunity for possible genetic assortment, posing a probable threat to global public health. To assess the potential risk of the IAV reassortants derived from co-circulation of these two AI subtypes, reverse genetics technology was used to generate a set of IAV reassortants carrying single genetic segments of clade 2.2.1.2 virus A/duck/Egypt/Q4596D/2012 (H5N1), a representative of the most prevalent H5N1 clade in Egypt, in the genetic backbone of A/chicken/Egypt/S4456B/2011 (H9N2), a representative of G1-like H9N2 lineage which is widely circulating in Egypt. Furthermore, the genetic compatibility, growth kinetics and virulence were evaluated in vitro in mammalian systems using the MDCK cell line and avian system using SPF embryonated chicken eggs. Pathogenicity and virus shedding were further tested using SPF chickens. Out of the eight desired H9-reassortants, we could rescue only 5 reassortant viruses, either due to difficulty in cloning (PB1 of H5N1 virus) or genetic incompatibility (NP-H5/H9 and NA-H5/H9). Results revealed higher replication rates for the H9N2 virus having the NS segment of H5N1 virus. The lowest survival rate in both SPF eggs and SPF chickens was associated with the H5N1 parent virus infection, followed by the HA-H5/H9 virus. Our findings also suggest that all other reassortant viruses were of lower pathogenicity than the wild type H5N1 virus.

Authors+Show Affiliations

Center of Scientific Excellence for Influenza Viruses, National Research Centre (NRC), El-Behouth Street, Dokki, Giza, 12622, Egypt.Center of Scientific Excellence for Influenza Viruses, National Research Centre (NRC), El-Behouth Street, Dokki, Giza, 12622, Egypt.Center of Scientific Excellence for Influenza Viruses, National Research Centre (NRC), El-Behouth Street, Dokki, Giza, 12622, Egypt.Pathology and Clinical Pathology Department, Faculty of Veterinary Medicine, Assuit University, Assuit, Egypt.Center of Scientific Excellence for Influenza Viruses, National Research Centre (NRC), El-Behouth Street, Dokki, Giza, 12622, Egypt.Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt.Center of Scientific Excellence for Influenza Viruses, National Research Centre (NRC), El-Behouth Street, Dokki, Giza, 12622, Egypt. mohamedahmedali2004@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28620809

Citation

Moatasim, Yassmin, et al. "Single Gene Reassortment of Highly Pathogenic Avian Influenza a H5N1 in the Low Pathogenic H9N2 Backbone and Its Impact On Pathogenicity and Infectivity of Novel Reassortant Viruses." Archives of Virology, vol. 162, no. 10, 2017, pp. 2959-2969.
Moatasim Y, Kandeil A, Mostafa A, et al. Single gene reassortment of highly pathogenic avian influenza A H5N1 in the low pathogenic H9N2 backbone and its impact on pathogenicity and infectivity of novel reassortant viruses. Arch Virol. 2017;162(10):2959-2969.
Moatasim, Y., Kandeil, A., Mostafa, A., Elghaffar, S. K. A., El Shesheny, R., Elwahy, A. H. M., & Ali, M. A. (2017). Single gene reassortment of highly pathogenic avian influenza A H5N1 in the low pathogenic H9N2 backbone and its impact on pathogenicity and infectivity of novel reassortant viruses. Archives of Virology, 162(10), 2959-2969. https://doi.org/10.1007/s00705-017-3434-x
Moatasim Y, et al. Single Gene Reassortment of Highly Pathogenic Avian Influenza a H5N1 in the Low Pathogenic H9N2 Backbone and Its Impact On Pathogenicity and Infectivity of Novel Reassortant Viruses. Arch Virol. 2017;162(10):2959-2969. PubMed PMID: 28620809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single gene reassortment of highly pathogenic avian influenza A H5N1 in the low pathogenic H9N2 backbone and its impact on pathogenicity and infectivity of novel reassortant viruses. AU - Moatasim,Yassmin, AU - Kandeil,Ahmed, AU - Mostafa,Ahmed, AU - Elghaffar,Sary Khaleel Abd, AU - El Shesheny,Rabeh, AU - Elwahy,Ahmed Helmy M, AU - Ali,Mohamed Ahmed, Y1 - 2017/06/15/ PY - 2017/03/11/received PY - 2017/05/02/accepted PY - 2017/6/18/pubmed PY - 2018/4/3/medline PY - 2017/6/17/entrez SP - 2959 EP - 2969 JF - Archives of virology JO - Arch. Virol. VL - 162 IS - 10 N2 - Avian influenza A H5N1 and H9N2 viruses have been extensively circulating in various avian species and frequently infect mammals, including humans. The synchronous circulation of both viruses in Egypt provides an opportunity for possible genetic assortment, posing a probable threat to global public health. To assess the potential risk of the IAV reassortants derived from co-circulation of these two AI subtypes, reverse genetics technology was used to generate a set of IAV reassortants carrying single genetic segments of clade 2.2.1.2 virus A/duck/Egypt/Q4596D/2012 (H5N1), a representative of the most prevalent H5N1 clade in Egypt, in the genetic backbone of A/chicken/Egypt/S4456B/2011 (H9N2), a representative of G1-like H9N2 lineage which is widely circulating in Egypt. Furthermore, the genetic compatibility, growth kinetics and virulence were evaluated in vitro in mammalian systems using the MDCK cell line and avian system using SPF embryonated chicken eggs. Pathogenicity and virus shedding were further tested using SPF chickens. Out of the eight desired H9-reassortants, we could rescue only 5 reassortant viruses, either due to difficulty in cloning (PB1 of H5N1 virus) or genetic incompatibility (NP-H5/H9 and NA-H5/H9). Results revealed higher replication rates for the H9N2 virus having the NS segment of H5N1 virus. The lowest survival rate in both SPF eggs and SPF chickens was associated with the H5N1 parent virus infection, followed by the HA-H5/H9 virus. Our findings also suggest that all other reassortant viruses were of lower pathogenicity than the wild type H5N1 virus. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/28620809/Single_gene_reassortment_of_highly_pathogenic_avian_influenza_A_H5N1_in_the_low_pathogenic_H9N2_backbone_and_its_impact_on_pathogenicity_and_infectivity_of_novel_reassortant_viruses_ L2 - https://dx.doi.org/10.1007/s00705-017-3434-x DB - PRIME DP - Unbound Medicine ER -