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Exposure to sublethal concentrations of Co3O4 and Mn2O3 nanoparticles induced elevated metal body burden in Daphnia magna.
Aquat Toxicol. 2017 Aug; 189:123-133.AT

Abstract

Despite the significant progress made in ecotoxicological research on nanoparticles (NPs), there is still very limited information available regarding the biological effects of certain types of NPs such as Co3O4 and Mn2O3. Only a couple of studies provide data on their impact on aquatic organisms whereas, alarmingly, these NPs have been proposed to have high toxicity potential. In addition, more data are needed to determine whether the adverse effects the metal NPs induce on aquatic organisms are rather due to their chemical or particulate nature. To address these open questions, the (sub)lethal effects of Co and Mn NPs in parallel with the respective soluble metal salts on Daphnia magna were studied. The aims of the current study were to i) assess the acute toxicity of Co3O4 and Mn2O3 NPs (primary size 10-30nm) to D. magna, ii) evaluate whether the acute NP exposure at sublethal concentrations influences D. magna post-exposure feeding behaviour and iii) quantify D. magna metal body burden after exposure and after the post-exposure feeding to estimate the potential of trophic transfer of metals. Flow cytometry and total reflection X-ray fluorescence spectroscopy were applied for feeding and metal body burden evaluations, respectively. CuO NPs (primary size 22-25nm) that are very toxic to D. magna were included in the study as a positive control. Since the release of metal ions is an important possibility for toxicity of metal NPs, soluble Co-, Mn- and Cu-salts were analysed in parallel. The solubilisation of Co3O4 NPs in the OECD202 assay conditions was 0.1% and Mn2O3 NPs 35%. Mn2O3 NPs also produced reactive oxygen species in abiotic conditions. However Co3O4 and Mn2O3 NPs were not acutely toxic to D. magna (48h EC50>100mg metal/L) at OECD202 assay conditions. The 48h EC50 values of soluble Co- and Mn-salts were 3.2mgCo/L and 41mgMn/L, respectively. Post-exposure feeding behaviour after 48h exposure to sublethal concentrations (≤10mg/L) of Co3O4 and Mn2O3 NPs differed from that of the unexposed (control) D. magna only at the highest exposure concentrations but was comparable to the feeding behaviour of the respective metal salt-exposed organisms. Upon 48h exposure, dose-dependent increase of D. magna total metal body burden in case of both the NPs and the soluble salts was observed. After 48h post-exposure feeding with algae C. reinhardtii (depuration): D. magna body burden remained elevated (up to 760-fold compared to the control organism) only in case of the NPs. This may indicate potential for trophic transfer of NPs/heavy metals and thus hazard for freshwater ecosystem.

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Authors+Show Affiliations

Heinlaan M
Environmental Biogeochemistry and Ecotoxicology, Department F.-A. Forel for Environmental and Aquatic Sciences, Faculty of Science, University of Geneva, Uni Carl Vogt, 66, Boulevard Carl-Vogt, CH-1211 Geneva 4, Switzerland; Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia. Electronic address: margit.heinlaan@kbfi.ee.
Muna M
Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia; Department of Materials and Environmental Technology, Tallinn University of Technology, Ehitajate tee 5, 19086 Tallinn, Estonia.
Juganson K
Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia; Department of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.
Oriekhova O
Environmental Physical Chemistry, Department F.-A. Forel for Environmental and Aquatic Sciences, Faculty of Science, University of Geneva, Uni Carl Vogt, 66, Boulevard Carl-Vogt, CH-1211 Geneva 4, Switzerland.
Stoll S
Environmental Physical Chemistry, Department F.-A. Forel for Environmental and Aquatic Sciences, Faculty of Science, University of Geneva, Uni Carl Vogt, 66, Boulevard Carl-Vogt, CH-1211 Geneva 4, Switzerland.
Kahru A
Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia; Estonian Academy of Sciences, Kohtu 6, 10130 Tallinn, Estonia.
Slaveykova VI
Environmental Biogeochemistry and Ecotoxicology, Department F.-A. Forel for Environmental and Aquatic Sciences, Faculty of Science, University of Geneva, Uni Carl Vogt, 66, Boulevard Carl-Vogt, CH-1211 Geneva 4, Switzerland. Electronic address: Vera.Slaveykova@unige.ch.

MeSH

AnimalsBody BurdenCobaltDaphniaDose-Response Relationship, DrugLethal Dose 50Manganese CompoundsMetal NanoparticlesOxidative StressOxidesParticle SizeReactive Oxygen SpeciesSolubilitySurface PropertiesToxicity Tests, AcuteWater Pollutants, Chemical

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28623688

Citation

Heinlaan, Margit, et al. "Exposure to Sublethal Concentrations of Co3O4 and Mn2O3 Nanoparticles Induced Elevated Metal Body Burden in Daphnia Magna." Aquatic Toxicology (Amsterdam, Netherlands), vol. 189, 2017, pp. 123-133.
Heinlaan M, Muna M, Juganson K, et al. Exposure to sublethal concentrations of Co3O4 and Mn2O3 nanoparticles induced elevated metal body burden in Daphnia magna. Aquat Toxicol. 2017;189:123-133.
Heinlaan, M., Muna, M., Juganson, K., Oriekhova, O., Stoll, S., Kahru, A., & Slaveykova, V. I. (2017). Exposure to sublethal concentrations of Co3O4 and Mn2O3 nanoparticles induced elevated metal body burden in Daphnia magna. Aquatic Toxicology (Amsterdam, Netherlands), 189, 123-133. https://doi.org/10.1016/j.aquatox.2017.06.002
Heinlaan M, et al. Exposure to Sublethal Concentrations of Co3O4 and Mn2O3 Nanoparticles Induced Elevated Metal Body Burden in Daphnia Magna. Aquat Toxicol. 2017;189:123-133. PubMed PMID: 28623688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exposure to sublethal concentrations of Co3O4 and Mn2O3 nanoparticles induced elevated metal body burden in Daphnia magna. AU - Heinlaan,Margit, AU - Muna,Marge, AU - Juganson,Katre, AU - Oriekhova,Olena, AU - Stoll,Serge, AU - Kahru,Anne, AU - Slaveykova,Vera I, Y1 - 2017/06/06/ PY - 2016/12/16/received PY - 2017/05/26/revised PY - 2017/06/03/accepted PY - 2017/6/18/pubmed PY - 2017/10/17/medline PY - 2017/6/18/entrez KW - Bioaccumulation KW - Feeding behaviour KW - Flow cytometry KW - Reactive oxygen species KW - TXRF SP - 123 EP - 133 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 189 N2 - Despite the significant progress made in ecotoxicological research on nanoparticles (NPs), there is still very limited information available regarding the biological effects of certain types of NPs such as Co3O4 and Mn2O3. Only a couple of studies provide data on their impact on aquatic organisms whereas, alarmingly, these NPs have been proposed to have high toxicity potential. In addition, more data are needed to determine whether the adverse effects the metal NPs induce on aquatic organisms are rather due to their chemical or particulate nature. To address these open questions, the (sub)lethal effects of Co and Mn NPs in parallel with the respective soluble metal salts on Daphnia magna were studied. The aims of the current study were to i) assess the acute toxicity of Co3O4 and Mn2O3 NPs (primary size 10-30nm) to D. magna, ii) evaluate whether the acute NP exposure at sublethal concentrations influences D. magna post-exposure feeding behaviour and iii) quantify D. magna metal body burden after exposure and after the post-exposure feeding to estimate the potential of trophic transfer of metals. Flow cytometry and total reflection X-ray fluorescence spectroscopy were applied for feeding and metal body burden evaluations, respectively. CuO NPs (primary size 22-25nm) that are very toxic to D. magna were included in the study as a positive control. Since the release of metal ions is an important possibility for toxicity of metal NPs, soluble Co-, Mn- and Cu-salts were analysed in parallel. The solubilisation of Co3O4 NPs in the OECD202 assay conditions was 0.1% and Mn2O3 NPs 35%. Mn2O3 NPs also produced reactive oxygen species in abiotic conditions. However Co3O4 and Mn2O3 NPs were not acutely toxic to D. magna (48h EC50>100mg metal/L) at OECD202 assay conditions. The 48h EC50 values of soluble Co- and Mn-salts were 3.2mgCo/L and 41mgMn/L, respectively. Post-exposure feeding behaviour after 48h exposure to sublethal concentrations (≤10mg/L) of Co3O4 and Mn2O3 NPs differed from that of the unexposed (control) D. magna only at the highest exposure concentrations but was comparable to the feeding behaviour of the respective metal salt-exposed organisms. Upon 48h exposure, dose-dependent increase of D. magna total metal body burden in case of both the NPs and the soluble salts was observed. After 48h post-exposure feeding with algae C. reinhardtii (depuration): D. magna body burden remained elevated (up to 760-fold compared to the control organism) only in case of the NPs. This may indicate potential for trophic transfer of NPs/heavy metals and thus hazard for freshwater ecosystem. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/28623688/Exposure_to_sublethal_concentrations_of_Co3O4_and_Mn2O3_nanoparticles_induced_elevated_metal_body_burden_in_Daphnia_magna_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(17)30156-X DB - PRIME DP - Unbound Medicine ER -