Tags

Type your tag names separated by a space and hit enter

Development and validation of KASP markers for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 in wheat.
Theor Appl Genet. 2017 Sep; 130(9):1867-1884.TA

Abstract

KEY MESSAGE

Greenbug and Hessian fly are important pests that decrease wheat production worldwide. We developed and validated breeder-friendly KASP markers for marker-assisted breeding to increase selection efficiency. Greenbug (Schizaphis graminum Rondani) and Hessian fly [Mayetiola destructor (Say)] are two major destructive insect pests of wheat (Triticum aestivum L.) throughout wheat production regions in the USA and worldwide. Greenbug and Hessian fly infestation can significantly reduce grain yield and quality. Breeding for resistance to these two pests using marker-assisted selection (MAS) is the most economical strategy to minimize losses. In this study, doubled haploid lines from the Synthetic W7984 × Opata M85 wheat reference population were used to construct linkage maps for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 with genotyping-by-sequencing (GBS) and 90K array-based single nucleotide polymorphism (SNP) marker data. Flanking markers were closely linked to Gb7 and H32 and were located on chromosome 7DL and 3DL, respectively. Gb7-linked markers (synopGBS773 and synopGBS1141) and H32-linked markers (synopGBS901 and IWB65911) were converted into Kompetitive Allele Specific PCR (KASP) assays for MAS in wheat breeding. In addition, comparative mapping identified syntenic regions in Brachypodium distachyon, rice (Oryza sativa), and sorghum (Sorghum bicolor) for Gb7 and H32 that can be used for fine mapping and map-based cloning of the genes. The KASP markers developed in this study are the first set of SNPs tightly linked to Gb7 and H32 and will be very useful for MAS in wheat breeding programs and future genetic studies of greenbug and Hessian fly resistance.

Authors+Show Affiliations

Texas A&M AgriLife Research, Amarillo, TX, 79106, USA.Texas A&M AgriLife Research, Amarillo, TX, 79106, USA.Texas A&M AgriLife Research, Amarillo, TX, 79106, USA. Department of Soil and Crop Science, Texas A&M University, College Station, TX, 77843, USA.USDA-ARS Wheat, Peanut and Other Field Crop Research Unit, Stillwater, OK, 74075, USA.USDA-ARS and Department of Entomology, Kansas State University, Manhattan, KS, 66506, USA.Texas A&M AgriLife Research, Amarillo, TX, 79106, USA.Texas A&M AgriLife Research, Amarillo, TX, 79106, USA.Department of Soil and Crop Science, Texas A&M University, College Station, TX, 77843, USA.Texas A&M AgriLife Research, Amarillo, TX, 79106, USA.Genomic and Bioinformatics Services, Texas A&M AgriLife Research, College Station, TX, 77845, USA.Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, 14853, USA.Texas A&M AgriLife Research, Amarillo, TX, 79106, USA. SLiu@ag.tamu.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28624908

Citation

Tan, Chor-Tee, et al. "Development and Validation of KASP Markers for the Greenbug Resistance Gene Gb7 and the Hessian Fly Resistance Gene H32 in Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 130, no. 9, 2017, pp. 1867-1884.
Tan CT, Yu H, Yang Y, et al. Development and validation of KASP markers for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 in wheat. Theor Appl Genet. 2017;130(9):1867-1884.
Tan, C. T., Yu, H., Yang, Y., Xu, X., Chen, M., Rudd, J. C., Xue, Q., Ibrahim, A. M. H., Garza, L., Wang, S., Sorrells, M. E., & Liu, S. (2017). Development and validation of KASP markers for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 in wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 130(9), 1867-1884. https://doi.org/10.1007/s00122-017-2930-4
Tan CT, et al. Development and Validation of KASP Markers for the Greenbug Resistance Gene Gb7 and the Hessian Fly Resistance Gene H32 in Wheat. Theor Appl Genet. 2017;130(9):1867-1884. PubMed PMID: 28624908.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of KASP markers for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 in wheat. AU - Tan,Chor-Tee, AU - Yu,Hangjin, AU - Yang,Yan, AU - Xu,Xiangyang, AU - Chen,Mingshun, AU - Rudd,Jackie C, AU - Xue,Qingwu, AU - Ibrahim,Amir M H, AU - Garza,Lisa, AU - Wang,Shichen, AU - Sorrells,Mark E, AU - Liu,Shuyu, Y1 - 2017/06/17/ PY - 2017/02/27/received PY - 2017/05/30/accepted PY - 2017/6/19/pubmed PY - 2018/4/17/medline PY - 2017/6/19/entrez SP - 1867 EP - 1884 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 130 IS - 9 N2 - KEY MESSAGE: Greenbug and Hessian fly are important pests that decrease wheat production worldwide. We developed and validated breeder-friendly KASP markers for marker-assisted breeding to increase selection efficiency. Greenbug (Schizaphis graminum Rondani) and Hessian fly [Mayetiola destructor (Say)] are two major destructive insect pests of wheat (Triticum aestivum L.) throughout wheat production regions in the USA and worldwide. Greenbug and Hessian fly infestation can significantly reduce grain yield and quality. Breeding for resistance to these two pests using marker-assisted selection (MAS) is the most economical strategy to minimize losses. In this study, doubled haploid lines from the Synthetic W7984 × Opata M85 wheat reference population were used to construct linkage maps for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 with genotyping-by-sequencing (GBS) and 90K array-based single nucleotide polymorphism (SNP) marker data. Flanking markers were closely linked to Gb7 and H32 and were located on chromosome 7DL and 3DL, respectively. Gb7-linked markers (synopGBS773 and synopGBS1141) and H32-linked markers (synopGBS901 and IWB65911) were converted into Kompetitive Allele Specific PCR (KASP) assays for MAS in wheat breeding. In addition, comparative mapping identified syntenic regions in Brachypodium distachyon, rice (Oryza sativa), and sorghum (Sorghum bicolor) for Gb7 and H32 that can be used for fine mapping and map-based cloning of the genes. The KASP markers developed in this study are the first set of SNPs tightly linked to Gb7 and H32 and will be very useful for MAS in wheat breeding programs and future genetic studies of greenbug and Hessian fly resistance. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/28624908/Development_and_validation_of_KASP_markers_for_the_greenbug_resistance_gene_Gb7_and_the_Hessian_fly_resistance_gene_H32_in_wheat_ L2 - https://dx.doi.org/10.1007/s00122-017-2930-4 DB - PRIME DP - Unbound Medicine ER -