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Validated UPLC-MS/MS method for simultaneous quantification of eight saikosaponins in rat plasma: Application to a comparative pharmacokinetic study in depression rats after oral administration of extracts of raw and vinegar-baked Bupleuri Radix.

Abstract

A sensitive and efficient UPLC-MS/MS method was developed and validated for simultaneous quantification of eight saikosaponins (SSa, SSb1, SSb2, SSb3, SSb4, SSc, SSd and SSf) in rat plasma. Furthermore, comparative pharmacokinetic profiles of these sakosaponins were investigated, following oral administration of extracts of raw and vinegar-baked Bupleuri Radix to depression rats. Biosamples were processed with liquid-liquid extraction technique using ethyl acetate. Chromatographic separation was accomplished on Waters BEH C18 UPLC column. 0.05% formic acid in water and acetonitrile as mobile phase was used at a flow rate of 0.40mL/min. The analytes and internal standard, digoxin, were detected using negative ion electrospray ionization in multiple reaction monitoring mode. The lower limit of quantification was less than 0.62ng/mL for the each analyte. The validation parameters investigated, which were specificity, precision, accuracy, matrix effect, recovery and stability, were well within acceptable limits. Results showed that for some of analytes, AUC0-t and Cmax were significantly different after oral administration of extracts of raw and vinegar-baked Bupleuri Radix. Moreover, the pharmacokinetic study in pathological state could provide more useful information to guide the clinical usage of Bupleuri Radix.

Authors+Show Affiliations

Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China. Electronic address: jincailu@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28628838

Citation

Lei, Tianli, et al. "Validated UPLC-MS/MS Method for Simultaneous Quantification of Eight Saikosaponins in Rat Plasma: Application to a Comparative Pharmacokinetic Study in Depression Rats After Oral Administration of Extracts of Raw and Vinegar-baked Bupleuri Radix." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1060, 2017, pp. 231-239.
Lei T, Zhang D, Guo K, et al. Validated UPLC-MS/MS method for simultaneous quantification of eight saikosaponins in rat plasma: Application to a comparative pharmacokinetic study in depression rats after oral administration of extracts of raw and vinegar-baked Bupleuri Radix. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1060:231-239.
Lei, T., Zhang, D., Guo, K., Li, M., Lv, C., Wang, J., ... Lu, J. (2017). Validated UPLC-MS/MS method for simultaneous quantification of eight saikosaponins in rat plasma: Application to a comparative pharmacokinetic study in depression rats after oral administration of extracts of raw and vinegar-baked Bupleuri Radix. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1060, pp. 231-239. doi:10.1016/j.jchromb.2017.06.007.
Lei T, et al. Validated UPLC-MS/MS Method for Simultaneous Quantification of Eight Saikosaponins in Rat Plasma: Application to a Comparative Pharmacokinetic Study in Depression Rats After Oral Administration of Extracts of Raw and Vinegar-baked Bupleuri Radix. J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Aug 15;1060:231-239. PubMed PMID: 28628838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated UPLC-MS/MS method for simultaneous quantification of eight saikosaponins in rat plasma: Application to a comparative pharmacokinetic study in depression rats after oral administration of extracts of raw and vinegar-baked Bupleuri Radix. AU - Lei,Tianli, AU - Zhang,Dandan, AU - Guo,Kun, AU - Li,Mingxiao, AU - Lv,Chongning, AU - Wang,Jing, AU - Jia,Lingyun, AU - Lu,Jincai, PY - 2017/01/30/received PY - 2017/06/01/revised PY - 2017/06/04/accepted PY - 2017/6/20/pubmed PY - 2017/9/16/medline PY - 2017/6/20/entrez KW - Bupleuri Radix KW - Depression KW - Pharmacokinetics KW - Vinegar-baked KW - :UPLC-MS/MS SP - 231 EP - 239 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 1060 N2 - A sensitive and efficient UPLC-MS/MS method was developed and validated for simultaneous quantification of eight saikosaponins (SSa, SSb1, SSb2, SSb3, SSb4, SSc, SSd and SSf) in rat plasma. Furthermore, comparative pharmacokinetic profiles of these sakosaponins were investigated, following oral administration of extracts of raw and vinegar-baked Bupleuri Radix to depression rats. Biosamples were processed with liquid-liquid extraction technique using ethyl acetate. Chromatographic separation was accomplished on Waters BEH C18 UPLC column. 0.05% formic acid in water and acetonitrile as mobile phase was used at a flow rate of 0.40mL/min. The analytes and internal standard, digoxin, were detected using negative ion electrospray ionization in multiple reaction monitoring mode. The lower limit of quantification was less than 0.62ng/mL for the each analyte. The validation parameters investigated, which were specificity, precision, accuracy, matrix effect, recovery and stability, were well within acceptable limits. Results showed that for some of analytes, AUC0-t and Cmax were significantly different after oral administration of extracts of raw and vinegar-baked Bupleuri Radix. Moreover, the pharmacokinetic study in pathological state could provide more useful information to guide the clinical usage of Bupleuri Radix. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/28628838/Validated_UPLC_MS/MS_method_for_simultaneous_quantification_of_eight_saikosaponins_in_rat_plasma:_Application_to_a_comparative_pharmacokinetic_study_in_depression_rats_after_oral_administration_of_extracts_of_raw_and_vinegar_baked_Bupleuri_Radix_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(17)30181-2 DB - PRIME DP - Unbound Medicine ER -