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Antibacterial activity of crude extracts of some South African medicinal plants against multidrug resistant etiological agents of diarrhoea.
BMC Complement Altern Med. 2017 Jun 19; 17(1):321.BC

Abstract

BACKGROUND

This study evaluated the antibacterial activity of some plants used in folklore medicine to treat diarrhoea in the Eastern Cape Province, South Africa.

METHODS

The acetone extracts of Acacia mearnsii De Wild., Aloe arborescens Mill., A. striata Haw., Cyathula uncinulata (Schrad.) Schinz, Eucomis autumnalis (Mill.) Chitt., E. comosa (Houtt.) Wehrh., Hermbstaedtia odorata (Burch. ex Moq.) T.Cooke, Hydnora africana Thunb, Hypoxis latifolia Wight, Pelargonium sidoides DC, Psidium guajava L and Schizocarphus nervosus (Burch.) van der Merwe were screened against Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, multi-resistant Salmonella enterica serovar Isangi, S. typhi, S. enterica serovar Typhimurium, Shigella flexneri type 1b and Sh. sonnei phase II. A qualitative phytochemical screening of the plants extracts was by thin layer chromatography. Plants extracts were screened for antibacterial activity using serial dilution microplate technique and bioautography.

RESULTS

The TLC fingerprint indicated the presence of terpenoids and flavonoids in the herbs. Most of the tested organisms were sensitive to the crude acetone extracts with minimum inhibitory concentration (MIC) values ranging from 0.018-2.5 mg/mℓ. Extracts of A. striata, C. uncinulata, E. autumnalis and P. guajava were more active against enteropathogens. S. aureus and Sh. flexneri were the most sensitive isolates to the crude extracts but of significance is the antibacterial activity of A. arborescens and P. guajava against a confirmed extended spectrum betalactamase positive S. enterica serovar Typhimurium.

CONCLUSION

The presence of bioactive compounds and the antibacterial activity of some of the selected herbs against multidrug resistant enteric agents corroborate assertions by traditional healers on their efficacies.

Authors+Show Affiliations

Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria. jumokade@yahoo.co.uk. Department of Medical Microbiology, Walter Sisulu University, Mthatha, 5117, South Africa. jumokade@yahoo.co.uk. SAMRC Microbial Water Quality, Monitoring Centre, University of Fort Hare, Alice, South Africa. jumokade@yahoo.co.uk.Directorate of Academic Affairs, University of Fort Hare, Alice, 5700, South Africa. SAMRC Microbial Water Quality, Monitoring Centre, University of Fort Hare, Alice, South Africa.Phytomedicine Programme, Department of Paraclinical Science, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa.Phytomedicine Programme, Department of Paraclinical Science, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa.SAMRC Microbial Water Quality, Monitoring Centre, University of Fort Hare, Alice, South Africa.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28629407

Citation

Bisi-Johnson, Mary A., et al. "Antibacterial Activity of Crude Extracts of some South African Medicinal Plants Against Multidrug Resistant Etiological Agents of Diarrhoea." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 321.
Bisi-Johnson MA, Obi CL, Samuel BB, et al. Antibacterial activity of crude extracts of some South African medicinal plants against multidrug resistant etiological agents of diarrhoea. BMC Complement Altern Med. 2017;17(1):321.
Bisi-Johnson, M. A., Obi, C. L., Samuel, B. B., Eloff, J. N., & Okoh, A. I. (2017). Antibacterial activity of crude extracts of some South African medicinal plants against multidrug resistant etiological agents of diarrhoea. BMC Complementary and Alternative Medicine, 17(1), 321. https://doi.org/10.1186/s12906-017-1802-4
Bisi-Johnson MA, et al. Antibacterial Activity of Crude Extracts of some South African Medicinal Plants Against Multidrug Resistant Etiological Agents of Diarrhoea. BMC Complement Altern Med. 2017 Jun 19;17(1):321. PubMed PMID: 28629407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial activity of crude extracts of some South African medicinal plants against multidrug resistant etiological agents of diarrhoea. AU - Bisi-Johnson,Mary A, AU - Obi,Chikwelu L, AU - Samuel,Babatunde B, AU - Eloff,Jacobus N, AU - Okoh,Anthony I, Y1 - 2017/06/19/ PY - 2016/10/14/received PY - 2017/05/22/accepted PY - 2017/6/21/entrez PY - 2017/6/21/pubmed PY - 2017/8/22/medline KW - Antibacterial KW - Bioautography KW - Diarrhoea KW - Medicinal plants KW - Phytochemical SP - 321 EP - 321 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: This study evaluated the antibacterial activity of some plants used in folklore medicine to treat diarrhoea in the Eastern Cape Province, South Africa. METHODS: The acetone extracts of Acacia mearnsii De Wild., Aloe arborescens Mill., A. striata Haw., Cyathula uncinulata (Schrad.) Schinz, Eucomis autumnalis (Mill.) Chitt., E. comosa (Houtt.) Wehrh., Hermbstaedtia odorata (Burch. ex Moq.) T.Cooke, Hydnora africana Thunb, Hypoxis latifolia Wight, Pelargonium sidoides DC, Psidium guajava L and Schizocarphus nervosus (Burch.) van der Merwe were screened against Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, multi-resistant Salmonella enterica serovar Isangi, S. typhi, S. enterica serovar Typhimurium, Shigella flexneri type 1b and Sh. sonnei phase II. A qualitative phytochemical screening of the plants extracts was by thin layer chromatography. Plants extracts were screened for antibacterial activity using serial dilution microplate technique and bioautography. RESULTS: The TLC fingerprint indicated the presence of terpenoids and flavonoids in the herbs. Most of the tested organisms were sensitive to the crude acetone extracts with minimum inhibitory concentration (MIC) values ranging from 0.018-2.5 mg/mℓ. Extracts of A. striata, C. uncinulata, E. autumnalis and P. guajava were more active against enteropathogens. S. aureus and Sh. flexneri were the most sensitive isolates to the crude extracts but of significance is the antibacterial activity of A. arborescens and P. guajava against a confirmed extended spectrum betalactamase positive S. enterica serovar Typhimurium. CONCLUSION: The presence of bioactive compounds and the antibacterial activity of some of the selected herbs against multidrug resistant enteric agents corroborate assertions by traditional healers on their efficacies. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28629407/Antibacterial_activity_of_crude_extracts_of_some_South_African_medicinal_plants_against_multidrug_resistant_etiological_agents_of_diarrhoea_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1802-4 DB - PRIME DP - Unbound Medicine ER -