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The antinociceptive effects of a standardized ethanol extract of the Bidens odorata Cav (Asteraceae) leaves are mediated by ATP-sensitive K+ channels.
J Ethnopharmacol. 2017 Jul 31; 207:30-33.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Bidens odorata Cav (Asteraceae) is used for the empirical treatment of inflammation and pain.

AIM OF THE STUDY

This work evaluated the in vitro and in vivo toxicity, antioxidant activity, as well as the anti-inflammatory and antinociceptive effects of an ethanol extract from Bidens odorata leaves (BOE).

MATERIALS AND METHODS

The in vitro toxicity of BOE (10-1000µg/ml) was evaluated with the comet assay in PBMC. The in vivo acute toxicity of BOE (500-5000mg/kg) and the effect of BOE (10-1000µg/ml) on the level of ROS in PBMC were determined. The in vivo anti-inflammatory activity of BOE was assessed using the TPA-induced ear edema in mice. The antinociceptive activities of BOE (50-200mg/kg p.o.) were assessed using the acetic acid and formalin tests. The antinociceptive mechanism of BOE was determined using naloxone and glibenclamide.

RESULTS

BOE lacked DNA damage, and showed low in vivo toxicity (LD50 > 5000mg/kg p.o.). BOE inhibited ROS production (IC50 = 252.13 ± 20.54µg/ml), and decreased inflammation by 36.1 ± 3.66%. In both antinociceptive test, BOE (200mg/kg) exerted activity with similar activity than the reference drugs.

CONCLUSION

B. odorata exerts low in vitro and in vivo toxicity, antioxidant effects, moderate in vivo anti-inflammatory activity, and antinociceptive effects mediated by ATP-sensitive K+ channels.

Authors+Show Affiliations

Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato,Guanajuato, Mexico. Electronic address: juan.zapata@ugto.mx.Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato,Guanajuato, Mexico.Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Metepec, Puebla, Mexico. Electronic address: irmafabiola@yahoo.com.Unidad Académica Multidisciplinaria de la Zona Huasteca, Universidad Autónoma de San Luis Potosí, Ciudad Valles, San Luis Potosí, Mexico.Escuela de Odontología, Universidad Cuauhtémoc plantel San Luis Potosí, San Luis Potosí, San Luis Potosí, Mexico.Unidad Académica Multidisciplinaria de la Zona Huasteca, Universidad Autónoma de San Luis Potosí, Ciudad Valles, San Luis Potosí, Mexico.Departamento de Química, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato, Mexico.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

28629817

Citation

Zapata-Morales, Juan Ramón, et al. "The Antinociceptive Effects of a Standardized Ethanol Extract of the Bidens Odorata Cav (Asteraceae) Leaves Are Mediated By ATP-sensitive K+ Channels." Journal of Ethnopharmacology, vol. 207, 2017, pp. 30-33.
Zapata-Morales JR, Alonso-Castro AJ, Domínguez F, et al. The antinociceptive effects of a standardized ethanol extract of the Bidens odorata Cav (Asteraceae) leaves are mediated by ATP-sensitive K+ channels. J Ethnopharmacol. 2017;207:30-33.
Zapata-Morales, J. R., Alonso-Castro, A. J., Domínguez, F., Carranza-Álvarez, C., Isiordia-Espinoza, M., Hernández-Morales, A., & Solorio-Alvarado, C. (2017). The antinociceptive effects of a standardized ethanol extract of the Bidens odorata Cav (Asteraceae) leaves are mediated by ATP-sensitive K+ channels. Journal of Ethnopharmacology, 207, 30-33. https://doi.org/10.1016/j.jep.2017.06.021
Zapata-Morales JR, et al. The Antinociceptive Effects of a Standardized Ethanol Extract of the Bidens Odorata Cav (Asteraceae) Leaves Are Mediated By ATP-sensitive K+ Channels. J Ethnopharmacol. 2017 Jul 31;207:30-33. PubMed PMID: 28629817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The antinociceptive effects of a standardized ethanol extract of the Bidens odorata Cav (Asteraceae) leaves are mediated by ATP-sensitive K+ channels. AU - Zapata-Morales,Juan Ramón, AU - Alonso-Castro,Angel Josabad, AU - Domínguez,Fabiola, AU - Carranza-Álvarez,Candy, AU - Isiordia-Espinoza,Mario, AU - Hernández-Morales,Alejandro, AU - Solorio-Alvarado,Cesar, Y1 - 2017/06/16/ PY - 2017/04/05/received PY - 2017/04/10/revised PY - 2017/06/13/accepted PY - 2017/6/21/pubmed PY - 2018/2/2/medline PY - 2017/6/21/entrez KW - Tramadol hydrochloride (PubChem CID:63013) KW - glibenclamide (PubChem CID:3488) KW - ketorolac (PubChem CID:3826) KW - naloxone (PubChem CID:5464092) KW - naproxen sodium (PubChem CID: 23681059) SP - 30 EP - 33 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 207 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Bidens odorata Cav (Asteraceae) is used for the empirical treatment of inflammation and pain. AIM OF THE STUDY: This work evaluated the in vitro and in vivo toxicity, antioxidant activity, as well as the anti-inflammatory and antinociceptive effects of an ethanol extract from Bidens odorata leaves (BOE). MATERIALS AND METHODS: The in vitro toxicity of BOE (10-1000µg/ml) was evaluated with the comet assay in PBMC. The in vivo acute toxicity of BOE (500-5000mg/kg) and the effect of BOE (10-1000µg/ml) on the level of ROS in PBMC were determined. The in vivo anti-inflammatory activity of BOE was assessed using the TPA-induced ear edema in mice. The antinociceptive activities of BOE (50-200mg/kg p.o.) were assessed using the acetic acid and formalin tests. The antinociceptive mechanism of BOE was determined using naloxone and glibenclamide. RESULTS: BOE lacked DNA damage, and showed low in vivo toxicity (LD50 > 5000mg/kg p.o.). BOE inhibited ROS production (IC50 = 252.13 ± 20.54µg/ml), and decreased inflammation by 36.1 ± 3.66%. In both antinociceptive test, BOE (200mg/kg) exerted activity with similar activity than the reference drugs. CONCLUSION: B. odorata exerts low in vitro and in vivo toxicity, antioxidant effects, moderate in vivo anti-inflammatory activity, and antinociceptive effects mediated by ATP-sensitive K+ channels. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/28629817/The_antinociceptive_effects_of_a_standardized_ethanol_extract_of_the_Bidens_odorata_Cav__Asteraceae__leaves_are_mediated_by_ATP_sensitive_K+_channels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(17)31338-7 DB - PRIME DP - Unbound Medicine ER -