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Emerging fungal pathogen Ophidiomyces ophiodiicola in wild European snakes.
Sci Rep. 2017 06 19; 7(1):3844.SR

Abstract

Snake fungal disease (SFD) is an emerging disease of conservation concern in eastern North America. Ophidiomyces ophiodiicola, the causative agent of SFD, has been isolated from over 30 species of wild snakes from six families in North America. Whilst O. ophiodiicola has been isolated from captive snakes outside North America, the pathogen has not been reported from wild snakes elsewhere. We screened 33 carcasses and 303 moulted skins from wild snakes collected from 2010-2016 in Great Britain and the Czech Republic for the presence of macroscopic skin lesions and O. ophiodiicola. The fungus was detected using real-time PCR in 26 (8.6%) specimens across the period of collection. Follow up culture and histopathologic analyses confirmed that both O. ophiodiicola and SFD occur in wild European snakes. Although skin lesions were mild in most cases, in some snakes they were severe and were considered likely to have contributed to mortality. Culture characterisations demonstrated that European isolates grew more slowly than those from the United States, and phylogenetic analyses indicated that isolates from European wild snakes reside in a clade distinct from the North American isolates examined. These genetic and phenotypic differences indicate that the European isolates represent novel strains of O. ophiodiicola. Further work is required to understand the individual and population level impact of this pathogen in Europe.

Authors+Show Affiliations

Institute of Zoology, Zoological Society of London, Regent's Park, London, NW1 4RY, UK. University College London, Gower Street, London, WC1E 6BT, UK.US Geological Survey-National Wildlife Health Center, Madison, Wisconsin 53711, USA.US Geological Survey-National Wildlife Health Center, Madison, Wisconsin 53711, USA.Institute of Zoology, Zoological Society of London, Regent's Park, London, NW1 4RY, UK. IDEXX Laboratories Limited, Grange House, Sandbeck Way, Wetherby, West Yorkshire, LS22 7DN, UK.Institute of Zoology, Zoological Society of London, Regent's Park, London, NW1 4RY, UK. School of Biosciences, Cardiff University, Cardiff, CF10 3AX, Wales, UK.Institute of Zoology, Zoological Society of London, Regent's Park, London, NW1 4RY, UK.Froglife, 1 Loxley, Peterborough, Cambridgeshire, PE4 5BW, UK. Conservation Science Group, Department of Zoology, University of Cambridge, The David Attenborough Building, Cambridgeshire, CB2 3QZ, UK.Institute of Zoology, Zoological Society of London, Regent's Park, London, NW1 4RY, UK. Naturemetrics, Unit 2 Littleton House, Littleton Road, Ashford, Surrey, TW15 1UU, UK.Public Health England, Myrtle Road, Bristol, BS2 8EL, UK.Department of Ecology and Diseases of Game, Fish and Bees, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.Institute of Zoology, Zoological Society of London, Regent's Park, London, NW1 4RY, UK.Institute of Zoology, Zoological Society of London, Regent's Park, London, NW1 4RY, UK. Becki.Lawson@ioz.ac.uk.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28630406

Citation

Franklinos, Lydia H V., et al. "Emerging Fungal Pathogen Ophidiomyces Ophiodiicola in Wild European Snakes." Scientific Reports, vol. 7, no. 1, 2017, p. 3844.
Franklinos LHV, Lorch JM, Bohuski E, et al. Emerging fungal pathogen Ophidiomyces ophiodiicola in wild European snakes. Sci Rep. 2017;7(1):3844.
Franklinos, L. H. V., Lorch, J. M., Bohuski, E., Rodriguez-Ramos Fernandez, J., Wright, O. N., Fitzpatrick, L., Petrovan, S., Durrant, C., Linton, C., Baláž, V., Cunningham, A. A., & Lawson, B. (2017). Emerging fungal pathogen Ophidiomyces ophiodiicola in wild European snakes. Scientific Reports, 7(1), 3844. https://doi.org/10.1038/s41598-017-03352-1
Franklinos LHV, et al. Emerging Fungal Pathogen Ophidiomyces Ophiodiicola in Wild European Snakes. Sci Rep. 2017 06 19;7(1):3844. PubMed PMID: 28630406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Emerging fungal pathogen Ophidiomyces ophiodiicola in wild European snakes. AU - Franklinos,Lydia H V, AU - Lorch,Jeffrey M, AU - Bohuski,Elizabeth, AU - Rodriguez-Ramos Fernandez,Julia, AU - Wright,Owen N, AU - Fitzpatrick,Liam, AU - Petrovan,Silviu, AU - Durrant,Chris, AU - Linton,Chris, AU - Baláž,Vojtech, AU - Cunningham,Andrew A, AU - Lawson,Becki, Y1 - 2017/06/19/ PY - 2017/02/23/received PY - 2017/04/26/accepted PY - 2017/6/21/entrez PY - 2017/6/21/pubmed PY - 2018/12/24/medline SP - 3844 EP - 3844 JF - Scientific reports JO - Sci Rep VL - 7 IS - 1 N2 - Snake fungal disease (SFD) is an emerging disease of conservation concern in eastern North America. Ophidiomyces ophiodiicola, the causative agent of SFD, has been isolated from over 30 species of wild snakes from six families in North America. Whilst O. ophiodiicola has been isolated from captive snakes outside North America, the pathogen has not been reported from wild snakes elsewhere. We screened 33 carcasses and 303 moulted skins from wild snakes collected from 2010-2016 in Great Britain and the Czech Republic for the presence of macroscopic skin lesions and O. ophiodiicola. The fungus was detected using real-time PCR in 26 (8.6%) specimens across the period of collection. Follow up culture and histopathologic analyses confirmed that both O. ophiodiicola and SFD occur in wild European snakes. Although skin lesions were mild in most cases, in some snakes they were severe and were considered likely to have contributed to mortality. Culture characterisations demonstrated that European isolates grew more slowly than those from the United States, and phylogenetic analyses indicated that isolates from European wild snakes reside in a clade distinct from the North American isolates examined. These genetic and phenotypic differences indicate that the European isolates represent novel strains of O. ophiodiicola. Further work is required to understand the individual and population level impact of this pathogen in Europe. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/28630406/Emerging_fungal_pathogen_Ophidiomyces_ophiodiicola_in_wild_European_snakes_ L2 - http://dx.doi.org/10.1038/s41598-017-03352-1 DB - PRIME DP - Unbound Medicine ER -