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Characterization of an orf virus isolate from an outbreak in Heilongjiang province, China.
Arch Virol 2017; 162(10):3143-3149AV

Abstract

Contagious ecthyma, caused by orf virus (ORFV), is an epitheliotrophic contagious disease with zoonotic implications that mainly affects sheep, goats, wild ruminants, and humans. Recently, a novel ORFV strain, OV/HLJ/04, was successfully isolated from the skin and mucosal lesions of a goat with severe clinical sore mouth symptoms in Heilongjiang province of China. The OV/HLJ/04 isolate was characterized by electron microscopy, serological tests, and experimental reproduction of disease. The purified virions exhibited a typical ovoid shape when observed by electron microscopy. Moreover, experimental reproduction of disease showed that a lamb developed typical clinical signs of contagious ecthyma, such as severe vascular proliferation, when inoculated with the virus. Subsequently, amplification of ORFV011 (B2L) gene fragments of viral DNA by polymerase chain reaction (PCR) and gene sequencing were performed. Phylogenetic analysis of the B2L protein gene revealed that this strain clusters with ORFV strains from epidemic-stricken areas worldwide, including recent mainland China isolates. Analysis using ClustalW MegAlign in DNAStar indicated that OV/HLJ/04 (GenBank: KU523790.1) was genetically closely related to the isolates Gansu (JQ904789), with 99.7% identity; NZ2 (DQ184476), with 97.4% identity; and Xinjiang (KF666560), with 90.6% identity. These results may provide insights into the genotype of the etiological agent responsible for the orf outbreak in Heilongjiang Province.

Authors+Show Affiliations

Virology Laboratory, College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, 2 Xinyang road, Daqing, 163319, China.Virology Laboratory, College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, 2 Xinyang road, Daqing, 163319, China.Virology Laboratory, College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, 2 Xinyang road, Daqing, 163319, China.Virology Laboratory, College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, 2 Xinyang road, Daqing, 163319, China.Virology Laboratory, College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, 2 Xinyang road, Daqing, 163319, China.Virology Laboratory, College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, 2 Xinyang road, Daqing, 163319, China.Virology Laboratory, Division of Animal Health, College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, 2 Xinyang road, Daqing, 163319, China.Virology Laboratory, College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, 2 Xinyang road, Daqing, 163319, China. cyd163163@126.com.

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

28631053

Citation

Yu, Yongzhong, et al. "Characterization of an Orf Virus Isolate From an Outbreak in Heilongjiang Province, China." Archives of Virology, vol. 162, no. 10, 2017, pp. 3143-3149.
Yu Y, Tan Q, Zhao W, et al. Characterization of an orf virus isolate from an outbreak in Heilongjiang province, China. Arch Virol. 2017;162(10):3143-3149.
Yu, Y., Tan, Q., Zhao, W., Zhang, X., Ma, J., Wu, Z., ... Cui, Y. (2017). Characterization of an orf virus isolate from an outbreak in Heilongjiang province, China. Archives of Virology, 162(10), pp. 3143-3149. doi:10.1007/s00705-017-3426-x.
Yu Y, et al. Characterization of an Orf Virus Isolate From an Outbreak in Heilongjiang Province, China. Arch Virol. 2017;162(10):3143-3149. PubMed PMID: 28631053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of an orf virus isolate from an outbreak in Heilongjiang province, China. AU - Yu,Yongzhong, AU - Tan,Qiang, AU - Zhao,Wenbo, AU - Zhang,Xue, AU - Ma,Jinzhu, AU - Wu,Zhijun, AU - Zhu,Zhanbo, AU - Cui,Yudong, Y1 - 2017/06/19/ PY - 2017/01/01/received PY - 2017/05/17/accepted PY - 2017/6/21/pubmed PY - 2018/4/3/medline PY - 2017/6/21/entrez SP - 3143 EP - 3149 JF - Archives of virology JO - Arch. Virol. VL - 162 IS - 10 N2 - Contagious ecthyma, caused by orf virus (ORFV), is an epitheliotrophic contagious disease with zoonotic implications that mainly affects sheep, goats, wild ruminants, and humans. Recently, a novel ORFV strain, OV/HLJ/04, was successfully isolated from the skin and mucosal lesions of a goat with severe clinical sore mouth symptoms in Heilongjiang province of China. The OV/HLJ/04 isolate was characterized by electron microscopy, serological tests, and experimental reproduction of disease. The purified virions exhibited a typical ovoid shape when observed by electron microscopy. Moreover, experimental reproduction of disease showed that a lamb developed typical clinical signs of contagious ecthyma, such as severe vascular proliferation, when inoculated with the virus. Subsequently, amplification of ORFV011 (B2L) gene fragments of viral DNA by polymerase chain reaction (PCR) and gene sequencing were performed. Phylogenetic analysis of the B2L protein gene revealed that this strain clusters with ORFV strains from epidemic-stricken areas worldwide, including recent mainland China isolates. Analysis using ClustalW MegAlign in DNAStar indicated that OV/HLJ/04 (GenBank: KU523790.1) was genetically closely related to the isolates Gansu (JQ904789), with 99.7% identity; NZ2 (DQ184476), with 97.4% identity; and Xinjiang (KF666560), with 90.6% identity. These results may provide insights into the genotype of the etiological agent responsible for the orf outbreak in Heilongjiang Province. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/28631053/Characterization_of_an_orf_virus_isolate_from_an_outbreak_in_Heilongjiang_province_China_ L2 - https://dx.doi.org/10.1007/s00705-017-3426-x DB - PRIME DP - Unbound Medicine ER -