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The CpxR/CpxA system contributes to Salmonella gold-resistance by controlling the GolS-dependent gesABC transcription.
Environ Microbiol. 2017 10; 19(10):4035-4044.EM

Abstract

Several regulatory systems contribute to bacterial resistance to heavy metals controlling the expression of factors required to eliminate the intoxicant and/or to repair the damage caused by it. In Salmonella, the response to Au ions is mediated by the specific metalloregulator GolS that, among other genes, controls the expression of the RND-efflux pump GesABC. In this work, we demonstrate that CpxR/CpxA, a main cell-envelope stress-responding system, promotes gesABC transcription in the presence of Au ions at neutral pH. Deletion of either cpxA or cpxR, or mutation of the CpxR-binding site identified upstream of the GolS-operator in the gesABC promoter region reduces but does not abrogate the GolS- and Au-dependent activation of gesABC. Au also triggers the activation of the CpxR/CpxA system and deletion of the cpxRA operon severely reduces survival in the presence of the toxic metal. Our results indicate that the coordinated action of GolS and CpxR/CpxA contribute to protecting the cell from severe Au damage.

Authors+Show Affiliations

Instituto de Biología Molecular y Celular de Rosario (IBR), Universidad Nacional de Rosario (UNR), CONICET y Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Ocampo y Esmeralda, Rosario, Argentina.Instituto de Biología Molecular y Celular de Rosario (IBR), Universidad Nacional de Rosario (UNR), CONICET y Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Ocampo y Esmeralda, Rosario, Argentina.Instituto de Biología Molecular y Celular de Rosario (IBR), Universidad Nacional de Rosario (UNR), CONICET y Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Ocampo y Esmeralda, Rosario, Argentina.Instituto de Biología Molecular y Celular de Rosario (IBR), Universidad Nacional de Rosario (UNR), CONICET y Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Ocampo y Esmeralda, Rosario, Argentina.Instituto de Biología Molecular y Celular de Rosario (IBR), Universidad Nacional de Rosario (UNR), CONICET y Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Ocampo y Esmeralda, Rosario, Argentina.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28631419

Citation

Cerminati, Sebastián, et al. "The CpxR/CpxA System Contributes to Salmonella Gold-resistance By Controlling the GolS-dependent gesABC Transcription." Environmental Microbiology, vol. 19, no. 10, 2017, pp. 4035-4044.
Cerminati S, Giri GF, Mendoza JI, et al. The CpxR/CpxA system contributes to Salmonella gold-resistance by controlling the GolS-dependent gesABC transcription. Environ Microbiol. 2017;19(10):4035-4044.
Cerminati, S., Giri, G. F., Mendoza, J. I., Soncini, F. C., & Checa, S. K. (2017). The CpxR/CpxA system contributes to Salmonella gold-resistance by controlling the GolS-dependent gesABC transcription. Environmental Microbiology, 19(10), 4035-4044. https://doi.org/10.1111/1462-2920.13837
Cerminati S, et al. The CpxR/CpxA System Contributes to Salmonella Gold-resistance By Controlling the GolS-dependent gesABC Transcription. Environ Microbiol. 2017;19(10):4035-4044. PubMed PMID: 28631419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The CpxR/CpxA system contributes to Salmonella gold-resistance by controlling the GolS-dependent gesABC transcription. AU - Cerminati,Sebastián, AU - Giri,Germán F, AU - Mendoza,Julián I, AU - Soncini,Fernando C, AU - Checa,Susana K, Y1 - 2017/07/17/ PY - 2016/08/04/received PY - 2017/06/14/revised PY - 2017/06/15/accepted PY - 2017/6/21/pubmed PY - 2018/1/31/medline PY - 2017/6/21/entrez SP - 4035 EP - 4044 JF - Environmental microbiology JO - Environ Microbiol VL - 19 IS - 10 N2 - Several regulatory systems contribute to bacterial resistance to heavy metals controlling the expression of factors required to eliminate the intoxicant and/or to repair the damage caused by it. In Salmonella, the response to Au ions is mediated by the specific metalloregulator GolS that, among other genes, controls the expression of the RND-efflux pump GesABC. In this work, we demonstrate that CpxR/CpxA, a main cell-envelope stress-responding system, promotes gesABC transcription in the presence of Au ions at neutral pH. Deletion of either cpxA or cpxR, or mutation of the CpxR-binding site identified upstream of the GolS-operator in the gesABC promoter region reduces but does not abrogate the GolS- and Au-dependent activation of gesABC. Au also triggers the activation of the CpxR/CpxA system and deletion of the cpxRA operon severely reduces survival in the presence of the toxic metal. Our results indicate that the coordinated action of GolS and CpxR/CpxA contribute to protecting the cell from severe Au damage. SN - 1462-2920 UR - https://www.unboundmedicine.com/medline/citation/28631419/The_CpxR/CpxA_system_contributes_to_Salmonella_gold_resistance_by_controlling_the_GolS_dependent_gesABC_transcription_ DB - PRIME DP - Unbound Medicine ER -