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Transcriptional and cellular effects of paracetamol in the oyster Crassostrea gigas.
Ecotoxicol Environ Saf. 2017 Oct; 144:258-267.EE

Abstract

Acetaminophen (paracetamol) (PAR) is one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) with analgesic and antipyretic properties consumed worldwide and often detected in the aquatic environment. Due to the fact that PAR induces oxidative stress in mammals, the aim of this study was to evaluate if similar effects were observed in oysters Crassostrea gigas, given their economic and ecological importance and worldwide distribution. Oysters were exposed for 1, 4 and 7 days to two different sublethal PAR concentrations (0, 1 and 100μgL-1). Cell viability, DNA damage in hemocytes and enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH) and glutathione S-transferases (GST) were evaluated in oyster gills. In addition, changes at transcriptional level of Cu/Zn superoxide dismutase (SOD), catalase-like (CAT-like), cytochrome P450 genes (CYP30C1, CYP2AU2, CYP3071A1, CYP356A1), glutathione S-transferase isoforms (GST-ω and GST-π-like), cyclooxygenase (COX), fatty acid binding proteins-like (FABP-like), and caspase genes were evaluated in oyster gills and digestive gland. No changes in cell viability and DNA damage were observed in oysters exposed to both PAR concentrations. Similarly, no significant changes were detected in the major antioxidant enzymes (except for auxiliary enzyme GR) in oyster gills, suggesting that changes in GR activity are enough to counteract a potential oxidative stress in C. gigas gills under these experimental conditions. Furthermore, changes at transcriptional level are concentration and tissue dependent. PAR elicited an inhibition of CYP30C1, CYP3071A1 and FABP-like transcripts highlighting their role in drug metabolism, transport and detoxification of PAR in the gills. GST transcript levels were type, tissue and concentration-dependent. GST-π-like was down-regulated in oyster gills exposed to the lowest PAR concentration and up-regulated in the digestive gland of oysters exposed to the highest PAR concentration. However, GST-ω transcript levels were lower only in oysters digestive gland exposed to the lowest PAR concentration. Therefore, changes at transcriptional level were more sensitive to assess the exposure to PAR at environmental relevant concentrations.

Authors+Show Affiliations

Laboratory of Biomarkers of Aquatic Contamination, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, SC 88040900, Brazil; CIMA, University of Algarve, Campus de Gambelas, 8000-139 Faro, Portugal.Laboratory of Biomarkers of Aquatic Contamination, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, SC 88040900, Brazil.Laboratory of Biomarkers of Aquatic Contamination, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, SC 88040900, Brazil.Laboratory of Biomarkers of Aquatic Contamination, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, SC 88040900, Brazil.Aquaculture Pathology Research Center-NEPAQ, Federal University of Santa Catarina, Florianópolis, Brazil.Laboratory of Biomarkers of Aquatic Contamination, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, SC 88040900, Brazil.Laboratory of Biomarkers of Aquatic Contamination, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, SC 88040900, Brazil.Laboratory of Biomarkers of Aquatic Contamination, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, SC 88040900, Brazil.Laboratory of Biomarkers of Aquatic Contamination, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, SC 88040900, Brazil.Aquaculture Pathology Research Center-NEPAQ, Federal University of Santa Catarina, Florianópolis, Brazil.Laboratory of Marine Mollusk, Department of Aquaculture, Center of Agricultural Science, Federal University of Santa Catarina, Florianopolis, SC 88040900, Brazil.Laboratory of Biomarkers of Aquatic Contamination, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, SC 88040900, Brazil. Electronic address: afonso.bainy@ufsc.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28633104

Citation

Bebianno, M J., et al. "Transcriptional and Cellular Effects of Paracetamol in the Oyster Crassostrea Gigas." Ecotoxicology and Environmental Safety, vol. 144, 2017, pp. 258-267.
Bebianno MJ, Mello ACP, Serrano MAS, et al. Transcriptional and cellular effects of paracetamol in the oyster Crassostrea gigas. Ecotoxicol Environ Saf. 2017;144:258-267.
Bebianno, M. J., Mello, A. C. P., Serrano, M. A. S., Flores-Nunes, F., Mattos, J. J., Zacchi, F. L., Piazza, C. E., Siebert, M. N., Piazza, R. S., Gomes, C. H. A. M., Melo, C. M. R., & Bainy, A. C. D. (2017). Transcriptional and cellular effects of paracetamol in the oyster Crassostrea gigas. Ecotoxicology and Environmental Safety, 144, 258-267. https://doi.org/10.1016/j.ecoenv.2017.06.034
Bebianno MJ, et al. Transcriptional and Cellular Effects of Paracetamol in the Oyster Crassostrea Gigas. Ecotoxicol Environ Saf. 2017;144:258-267. PubMed PMID: 28633104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional and cellular effects of paracetamol in the oyster Crassostrea gigas. AU - Bebianno,M J, AU - Mello,A C P, AU - Serrano,M A S, AU - Flores-Nunes,F, AU - Mattos,J J, AU - Zacchi,F L, AU - Piazza,C E, AU - Siebert,M N, AU - Piazza,R S, AU - Gomes,C H A M, AU - Melo,C M R, AU - Bainy,A C D, Y1 - 2017/06/20/ PY - 2017/04/11/received PY - 2017/06/08/revised PY - 2017/06/12/accepted PY - 2017/6/21/pubmed PY - 2017/11/14/medline PY - 2017/6/21/entrez KW - Antioxidant enzymes KW - Crassostrea gigas KW - Gene transcription KW - Paracetamol KW - Pharmaceuticals SP - 258 EP - 267 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 144 N2 - Acetaminophen (paracetamol) (PAR) is one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) with analgesic and antipyretic properties consumed worldwide and often detected in the aquatic environment. Due to the fact that PAR induces oxidative stress in mammals, the aim of this study was to evaluate if similar effects were observed in oysters Crassostrea gigas, given their economic and ecological importance and worldwide distribution. Oysters were exposed for 1, 4 and 7 days to two different sublethal PAR concentrations (0, 1 and 100μgL-1). Cell viability, DNA damage in hemocytes and enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH) and glutathione S-transferases (GST) were evaluated in oyster gills. In addition, changes at transcriptional level of Cu/Zn superoxide dismutase (SOD), catalase-like (CAT-like), cytochrome P450 genes (CYP30C1, CYP2AU2, CYP3071A1, CYP356A1), glutathione S-transferase isoforms (GST-ω and GST-π-like), cyclooxygenase (COX), fatty acid binding proteins-like (FABP-like), and caspase genes were evaluated in oyster gills and digestive gland. No changes in cell viability and DNA damage were observed in oysters exposed to both PAR concentrations. Similarly, no significant changes were detected in the major antioxidant enzymes (except for auxiliary enzyme GR) in oyster gills, suggesting that changes in GR activity are enough to counteract a potential oxidative stress in C. gigas gills under these experimental conditions. Furthermore, changes at transcriptional level are concentration and tissue dependent. PAR elicited an inhibition of CYP30C1, CYP3071A1 and FABP-like transcripts highlighting their role in drug metabolism, transport and detoxification of PAR in the gills. GST transcript levels were type, tissue and concentration-dependent. GST-π-like was down-regulated in oyster gills exposed to the lowest PAR concentration and up-regulated in the digestive gland of oysters exposed to the highest PAR concentration. However, GST-ω transcript levels were lower only in oysters digestive gland exposed to the lowest PAR concentration. Therefore, changes at transcriptional level were more sensitive to assess the exposure to PAR at environmental relevant concentrations. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/28633104/Transcriptional_and_cellular_effects_of_paracetamol_in_the_oyster_Crassostrea_gigas_ DB - PRIME DP - Unbound Medicine ER -